Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family

Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To eva...
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Autor*in:	Hou, Bingqian [verfasserIn] Wang, Dawei [verfasserIn] Yan, Fangfang [verfasserIn] Cheng, Xinxin [verfasserIn] Xu, Yongchang [verfasserIn] Xi, Xuepeng [verfasserIn] Ge, Wenyang [verfasserIn] Sun, Silong [verfasserIn] Su, Peisen [verfasserIn] Zhao, Lanfei [verfasserIn] Lyu, Zhongfan [verfasserIn] Hao, Yongchao [verfasserIn] Wang, Hongwei [verfasserIn] Kong, Lingrang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism

Übergeordnetes Werk:	Enthalten in: Food chemistry - New York, NY [u.a.] : Elsevier, 1976, 439
Übergeordnetes Werk:	volume:439

DOI / URN:	10.1016/j.foodchem.2023.138057

Katalog-ID:	ELV066372747

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245	1	0	\|a Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
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520			\|a Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin.
650		4	\|a Trichothecenes (TCNs)
650		4	\|a Detoxification
650		4	\|a Cytotoxicity
650		4	\|a BALB/c mice
650		4	\|a Metabolism
700	1		\|a Wang, Dawei \|e verfasserin \|4 aut
700	1		\|a Yan, Fangfang \|e verfasserin \|4 aut
700	1		\|a Cheng, Xinxin \|e verfasserin \|4 aut
700	1		\|a Xu, Yongchang \|e verfasserin \|4 aut
700	1		\|a Xi, Xuepeng \|e verfasserin \|4 aut
700	1		\|a Ge, Wenyang \|e verfasserin \|4 aut
700	1		\|a Sun, Silong \|e verfasserin \|0 (orcid)0000-0002-4541-9126 \|4 aut
700	1		\|a Su, Peisen \|e verfasserin \|4 aut
700	1		\|a Zhao, Lanfei \|e verfasserin \|0 (orcid)0000-0003-0139-1325 \|4 aut
700	1		\|a Lyu, Zhongfan \|e verfasserin \|4 aut
700	1		\|a Hao, Yongchao \|e verfasserin \|4 aut
700	1		\|a Wang, Hongwei \|e verfasserin \|4 aut
700	1		\|a Kong, Lingrang \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Food chemistry \|d New York, NY [u.a.] : Elsevier, 1976 \|g 439 \|h Online-Ressource \|w (DE-627)300898509 \|w (DE-600)1483647-6 \|w (DE-576)098330225 \|x 1873-7072 \|7 nnns
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912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2007
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912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2044
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912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
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912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
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936	b	k	\|a 58.34 \|j Lebensmitteltechnologie \|q VZ
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allfields	10.1016/j.foodchem.2023.138057 doi (DE-627)ELV066372747 (ELSEVIER)S0308-8146(23)02675-4 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Hou, Bingqian verfasserin aut Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin. Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism Wang, Dawei verfasserin aut Yan, Fangfang verfasserin aut Cheng, Xinxin verfasserin aut Xu, Yongchang verfasserin aut Xi, Xuepeng verfasserin aut Ge, Wenyang verfasserin aut Sun, Silong verfasserin (orcid)0000-0002-4541-9126 aut Su, Peisen verfasserin aut Zhao, Lanfei verfasserin (orcid)0000-0003-0139-1325 aut Lyu, Zhongfan verfasserin aut Hao, Yongchao verfasserin aut Wang, Hongwei verfasserin aut Kong, Lingrang verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 439 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:439 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 439
spelling	10.1016/j.foodchem.2023.138057 doi (DE-627)ELV066372747 (ELSEVIER)S0308-8146(23)02675-4 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Hou, Bingqian verfasserin aut Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin. Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism Wang, Dawei verfasserin aut Yan, Fangfang verfasserin aut Cheng, Xinxin verfasserin aut Xu, Yongchang verfasserin aut Xi, Xuepeng verfasserin aut Ge, Wenyang verfasserin aut Sun, Silong verfasserin (orcid)0000-0002-4541-9126 aut Su, Peisen verfasserin aut Zhao, Lanfei verfasserin (orcid)0000-0003-0139-1325 aut Lyu, Zhongfan verfasserin aut Hao, Yongchao verfasserin aut Wang, Hongwei verfasserin aut Kong, Lingrang verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 439 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:439 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 439
allfields_unstemmed	10.1016/j.foodchem.2023.138057 doi (DE-627)ELV066372747 (ELSEVIER)S0308-8146(23)02675-4 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Hou, Bingqian verfasserin aut Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin. Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism Wang, Dawei verfasserin aut Yan, Fangfang verfasserin aut Cheng, Xinxin verfasserin aut Xu, Yongchang verfasserin aut Xi, Xuepeng verfasserin aut Ge, Wenyang verfasserin aut Sun, Silong verfasserin (orcid)0000-0002-4541-9126 aut Su, Peisen verfasserin aut Zhao, Lanfei verfasserin (orcid)0000-0003-0139-1325 aut Lyu, Zhongfan verfasserin aut Hao, Yongchao verfasserin aut Wang, Hongwei verfasserin aut Kong, Lingrang verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 439 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:439 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 439
allfieldsGer	10.1016/j.foodchem.2023.138057 doi (DE-627)ELV066372747 (ELSEVIER)S0308-8146(23)02675-4 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Hou, Bingqian verfasserin aut Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin. Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism Wang, Dawei verfasserin aut Yan, Fangfang verfasserin aut Cheng, Xinxin verfasserin aut Xu, Yongchang verfasserin aut Xi, Xuepeng verfasserin aut Ge, Wenyang verfasserin aut Sun, Silong verfasserin (orcid)0000-0002-4541-9126 aut Su, Peisen verfasserin aut Zhao, Lanfei verfasserin (orcid)0000-0003-0139-1325 aut Lyu, Zhongfan verfasserin aut Hao, Yongchao verfasserin aut Wang, Hongwei verfasserin aut Kong, Lingrang verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 439 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:439 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 439
allfieldsSound	10.1016/j.foodchem.2023.138057 doi (DE-627)ELV066372747 (ELSEVIER)S0308-8146(23)02675-4 DE-627 ger DE-627 rda eng 540 660 VZ 58.34 bkl Hou, Bingqian verfasserin aut Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin. Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism Wang, Dawei verfasserin aut Yan, Fangfang verfasserin aut Cheng, Xinxin verfasserin aut Xu, Yongchang verfasserin aut Xi, Xuepeng verfasserin aut Ge, Wenyang verfasserin aut Sun, Silong verfasserin (orcid)0000-0002-4541-9126 aut Su, Peisen verfasserin aut Zhao, Lanfei verfasserin (orcid)0000-0003-0139-1325 aut Lyu, Zhongfan verfasserin aut Hao, Yongchao verfasserin aut Wang, Hongwei verfasserin aut Kong, Lingrang verfasserin aut Enthalten in Food chemistry New York, NY [u.a.] : Elsevier, 1976 439 Online-Ressource (DE-627)300898509 (DE-600)1483647-6 (DE-576)098330225 1873-7072 nnns volume:439 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 439
language	English
source	Enthalten in Food chemistry 439 volume:439
sourceStr	Enthalten in Food chemistry 439 volume:439
format_phy_str_mv	Article
bklname	Lebensmitteltechnologie
institution	findex.gbv.de
topic_facet	Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism
dewey-raw	540
isfreeaccess_bool	false
container_title	Food chemistry
authorswithroles_txt_mv	Hou, Bingqian @@aut@@ Wang, Dawei @@aut@@ Yan, Fangfang @@aut@@ Cheng, Xinxin @@aut@@ Xu, Yongchang @@aut@@ Xi, Xuepeng @@aut@@ Ge, Wenyang @@aut@@ Sun, Silong @@aut@@ Su, Peisen @@aut@@ Zhao, Lanfei @@aut@@ Lyu, Zhongfan @@aut@@ Hao, Yongchao @@aut@@ Wang, Hongwei @@aut@@ Kong, Lingrang @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	300898509
dewey-sort	3540
id	ELV066372747
language_de	englisch
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author	Hou, Bingqian
spellingShingle	Hou, Bingqian ddc 540 bkl 58.34 misc Trichothecenes (TCNs) misc Detoxification misc Cytotoxicity misc BALB/c mice misc Metabolism Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
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topic_title	540 660 VZ 58.34 bkl Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family Trichothecenes (TCNs) Detoxification Cytotoxicity BALB/c mice Metabolism
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title	Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
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title_full	Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
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author_browse	Hou, Bingqian Wang, Dawei Yan, Fangfang Cheng, Xinxin Xu, Yongchang Xi, Xuepeng Ge, Wenyang Sun, Silong Su, Peisen Zhao, Lanfei Lyu, Zhongfan Hao, Yongchao Wang, Hongwei Kong, Lingrang
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title_sort	fhb7-gst catalyzed glutathionylation effectively detoxifies the trichothecene family
title_auth	Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
abstract	Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin.
abstractGer	Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin.
abstract_unstemmed	Trichothecene (TCN) contamination in food and feed is a serious challenge due to the negative health and economic impacts. Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. These results demonstrate the promise of Fhb7-GST as a candidate of detoxification enzyme potentially applied in TCN-contaminated agricultural samples, minimizing the detrimental effects of the mycotoxin.
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title_short	Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family
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author2	Wang, Dawei Yan, Fangfang Cheng, Xinxin Xu, Yongchang Xi, Xuepeng Ge, Wenyang Sun, Silong Su, Peisen Zhao, Lanfei Lyu, Zhongfan Hao, Yongchao Wang, Hongwei Kong, Lingrang
author2Str	Wang, Dawei Yan, Fangfang Cheng, Xinxin Xu, Yongchang Xi, Xuepeng Ge, Wenyang Sun, Silong Su, Peisen Zhao, Lanfei Lyu, Zhongfan Hao, Yongchao Wang, Hongwei Kong, Lingrang
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up_date	2024-07-06T17:31:33.125Z
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Here, we confirmed that the glutathione S-transferase (GST) Fhb7-GST could broadly catalyze type A, type B and type D TCNs into glutathione epoxide adducts (TCN-13-GSHs). To evaluate the toxicity of TCN-13-GSH adducts, we performed cell proliferation assays in vitro, which demonstrated decreased cytotoxicity of the adducts. Moreover, in vivo assays (repeated-dose treatment in mice) confirmed that TCN-13-GSH adducts were dramatically less toxic than the corresponding TCNs. To establish whether TCN-13-GSH was metabolized back to free toxin during digestion, single-dose metabolic tests were performed in rats; DON-13-GSH was not hydrolyzed in vivo, but rather was quickly metabolized to another low-toxicity compound, DON-13-N-acetylcysteine. 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Fhb7-GST catalyzed glutathionylation effectively detoxifies the trichothecene family

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