In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument
Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Yuezhu [verfasserIn] Zhang, Yichi [verfasserIn] Wang, Junsheng [verfasserIn] Liu, Weibing [verfasserIn] Wang, Huan [verfasserIn] Song, Mingzhu [verfasserIn] Zhang, Hongyue [verfasserIn] Wang, Xin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2024 |
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| Schlagwörter: |
Microfluidic image flow cytometry |
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| Übergeordnetes Werk: |
Enthalten in: Ecotoxicology and environmental safety - Amsterdam : Elsevier, 1977, 270 |
|---|---|
| Übergeordnetes Werk: |
volume:270 |
| DOI / URN: |
10.1016/j.ecoenv.2023.115894 |
|---|
| Katalog-ID: |
ELV06666280X |
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| 245 | 1 | 0 | |a In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
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| 520 | |a Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. | ||
| 650 | 4 | |a Microfluidic image flow cytometry | |
| 650 | 4 | |a Bidirectional background subtraction | |
| 650 | 4 | |a Algal toxins | |
| 650 | 4 | |a Toxic effects | |
| 700 | 1 | |a Zhang, Yichi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Junsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Weibing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Mingzhu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hongyue |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Ecotoxicology and environmental safety |d Amsterdam : Elsevier, 1977 |g 270 |h Online-Ressource |w (DE-627)266018467 |w (DE-600)1466969-9 |w (DE-576)104193719 |x 1090-2414 |7 nnns |
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10.1016/j.ecoenv.2023.115894 doi (DE-627)ELV06666280X (ELSEVIER)S0147-6513(23)01398-2 DE-627 ger DE-627 rda eng 610 VZ 44.13 bkl Wang, Yuezhu verfasserin aut In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument 2024 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects Zhang, Yichi verfasserin aut Wang, Junsheng verfasserin aut Liu, Weibing verfasserin aut Wang, Huan verfasserin aut Song, Mingzhu verfasserin aut Zhang, Hongyue verfasserin aut Wang, Xin verfasserin aut Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 270 Online-Ressource (DE-627)266018467 (DE-600)1466969-9 (DE-576)104193719 1090-2414 nnns volume:270 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.13 Medizinische Ökologie VZ AR 270 |
| spelling |
10.1016/j.ecoenv.2023.115894 doi (DE-627)ELV06666280X (ELSEVIER)S0147-6513(23)01398-2 DE-627 ger DE-627 rda eng 610 VZ 44.13 bkl Wang, Yuezhu verfasserin aut In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument 2024 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects Zhang, Yichi verfasserin aut Wang, Junsheng verfasserin aut Liu, Weibing verfasserin aut Wang, Huan verfasserin aut Song, Mingzhu verfasserin aut Zhang, Hongyue verfasserin aut Wang, Xin verfasserin aut Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 270 Online-Ressource (DE-627)266018467 (DE-600)1466969-9 (DE-576)104193719 1090-2414 nnns volume:270 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.13 Medizinische Ökologie VZ AR 270 |
| allfields_unstemmed |
10.1016/j.ecoenv.2023.115894 doi (DE-627)ELV06666280X (ELSEVIER)S0147-6513(23)01398-2 DE-627 ger DE-627 rda eng 610 VZ 44.13 bkl Wang, Yuezhu verfasserin aut In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument 2024 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects Zhang, Yichi verfasserin aut Wang, Junsheng verfasserin aut Liu, Weibing verfasserin aut Wang, Huan verfasserin aut Song, Mingzhu verfasserin aut Zhang, Hongyue verfasserin aut Wang, Xin verfasserin aut Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 270 Online-Ressource (DE-627)266018467 (DE-600)1466969-9 (DE-576)104193719 1090-2414 nnns volume:270 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.13 Medizinische Ökologie VZ AR 270 |
| allfieldsGer |
10.1016/j.ecoenv.2023.115894 doi (DE-627)ELV06666280X (ELSEVIER)S0147-6513(23)01398-2 DE-627 ger DE-627 rda eng 610 VZ 44.13 bkl Wang, Yuezhu verfasserin aut In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument 2024 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects Zhang, Yichi verfasserin aut Wang, Junsheng verfasserin aut Liu, Weibing verfasserin aut Wang, Huan verfasserin aut Song, Mingzhu verfasserin aut Zhang, Hongyue verfasserin aut Wang, Xin verfasserin aut Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 270 Online-Ressource (DE-627)266018467 (DE-600)1466969-9 (DE-576)104193719 1090-2414 nnns volume:270 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.13 Medizinische Ökologie VZ AR 270 |
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10.1016/j.ecoenv.2023.115894 doi (DE-627)ELV06666280X (ELSEVIER)S0147-6513(23)01398-2 DE-627 ger DE-627 rda eng 610 VZ 44.13 bkl Wang, Yuezhu verfasserin aut In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument 2024 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects Zhang, Yichi verfasserin aut Wang, Junsheng verfasserin aut Liu, Weibing verfasserin aut Wang, Huan verfasserin aut Song, Mingzhu verfasserin aut Zhang, Hongyue verfasserin aut Wang, Xin verfasserin aut Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 270 Online-Ressource (DE-627)266018467 (DE-600)1466969-9 (DE-576)104193719 1090-2414 nnns volume:270 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 44.13 Medizinische Ökologie VZ AR 270 |
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610 VZ 44.13 bkl In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument Microfluidic image flow cytometry Bidirectional background subtraction Algal toxins Toxic effects |
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In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
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In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
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Wang, Yuezhu |
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Ecotoxicology and environmental safety |
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Wang, Yuezhu Zhang, Yichi Wang, Junsheng Liu, Weibing Wang, Huan Song, Mingzhu Zhang, Hongyue Wang, Xin |
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Wang, Yuezhu |
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10.1016/j.ecoenv.2023.115894 |
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in situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
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In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
| abstract |
Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. |
| abstractGer |
Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. |
| abstract_unstemmed |
Algal toxins produced by microalgae, such as domoic acid (DA) 1 1 (DA) domoic acid , have toxic effects on humans. However, toxicity tests using mice only yield lethal doses of algal toxins without providing insights into the mechanism of action on cells. In this study, a fast segmentation of microfluidic flow cytometry cell images based on the bidirectional background subtraction (BBS) 2 2 (BBS) bidirectional background subtraction method was developed to get the visual evidence of apoptosis in both bright-field and fluorescence images. This approach enables mapping of changes in cell morphology and activity under algal toxins, allowing for fast (within 60 s) and automated biological detection. By combining microfluidics with flow cytometry, the intricate cellular-level reaction process can be observed in micro samples of 293 T cells and mouse spleen cells, offering potential for future in vitro experiments. |
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In situ monitoring of toxic effects of algal toxin on cells by a novel microfluidic flow cytometry instrument |
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Zhang, Yichi Wang, Junsheng Liu, Weibing Wang, Huan Song, Mingzhu Zhang, Hongyue Wang, Xin |
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