Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification

The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, sca...
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Autor*in:	Cecconello, Alessandro [verfasserIn] Tonolo, Federica [verfasserIn] Rilievo, Graziano [verfasserIn] Molinari, Simone [verfasserIn] Talpe, Arthur [verfasserIn] Cozza, Giorgio [verfasserIn] Venerando, Andrea [verfasserIn] Kariyawasam, Indipalage Dinusha Harshani [verfasserIn] Govardhan, Gayathri Tiruchi [verfasserIn] Arusei, Ruth Jepchirchir [verfasserIn] Magro, Massimiliano [verfasserIn] Vianello, Fabio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification

Übergeordnetes Werk:	Enthalten in: Colloids and surfaces / B - Amsterdam [u.a.] : Elsevier Science, 1993, 234
Übergeordnetes Werk:	volume:234

DOI / URN:	10.1016/j.colsurfb.2023.113700

Katalog-ID:	ELV066884233

Internformat


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100	1		\|a Cecconello, Alessandro \|e verfasserin \|4 aut
245	1	0	\|a Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
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520			\|a The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility.
650		4	\|a Bioinspired nanomaterials
650		4	\|a Lactoferrin
650		4	\|a DNA
650		4	\|a Protein recognition
650		4	\|a Protein-DNA interaction
650		4	\|a Superparamagnetic nanoparticles
650		4	\|a Magnetic purification
700	1		\|a Tonolo, Federica \|e verfasserin \|0 (orcid)0000-0002-7780-8994 \|4 aut
700	1		\|a Rilievo, Graziano \|e verfasserin \|0 (orcid)0000-0003-4098-5636 \|4 aut
700	1		\|a Molinari, Simone \|e verfasserin \|4 aut
700	1		\|a Talpe, Arthur \|e verfasserin \|0 (orcid)0009-0009-5257-9820 \|4 aut
700	1		\|a Cozza, Giorgio \|e verfasserin \|4 aut
700	1		\|a Venerando, Andrea \|e verfasserin \|0 (orcid)0000-0003-0379-2309 \|4 aut
700	1		\|a Kariyawasam, Indipalage Dinusha Harshani \|e verfasserin \|4 aut
700	1		\|a Govardhan, Gayathri Tiruchi \|e verfasserin \|4 aut
700	1		\|a Arusei, Ruth Jepchirchir \|e verfasserin \|4 aut
700	1		\|a Magro, Massimiliano \|e verfasserin \|0 (orcid)0000-0002-4644-004X \|4 aut
700	1		\|a Vianello, Fabio \|e verfasserin \|0 (orcid)0000-0002-4874-7205 \|4 aut
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912			\|a GBV_ILN_4313
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allfields	10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234
spelling	10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234
allfields_unstemmed	10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234
allfieldsGer	10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234
allfieldsSound	10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234
language	English
source	Enthalten in Colloids and surfaces / B 234 volume:234
sourceStr	Enthalten in Colloids and surfaces / B 234 volume:234
format_phy_str_mv	Article
bklname	Zellbiologie
institution	findex.gbv.de
topic_facet	Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification
dewey-raw	540
isfreeaccess_bool	false
container_title	Colloids and surfaces / B
authorswithroles_txt_mv	Cecconello, Alessandro @@aut@@ Tonolo, Federica @@aut@@ Rilievo, Graziano @@aut@@ Molinari, Simone @@aut@@ Talpe, Arthur @@aut@@ Cozza, Giorgio @@aut@@ Venerando, Andrea @@aut@@ Kariyawasam, Indipalage Dinusha Harshani @@aut@@ Govardhan, Gayathri Tiruchi @@aut@@ Arusei, Ruth Jepchirchir @@aut@@ Magro, Massimiliano @@aut@@ Vianello, Fabio @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	306660016
dewey-sort	3540
id	ELV066884233
language_de	englisch
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author	Cecconello, Alessandro
spellingShingle	Cecconello, Alessandro ddc 540 bkl 42.15 misc Bioinspired nanomaterials misc Lactoferrin misc DNA misc Protein recognition misc Protein-DNA interaction misc Superparamagnetic nanoparticles misc Magnetic purification Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
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topic_title	540 VZ 42.15 bkl Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification
topic	ddc 540 bkl 42.15 misc Bioinspired nanomaterials misc Lactoferrin misc DNA misc Protein recognition misc Protein-DNA interaction misc Superparamagnetic nanoparticles misc Magnetic purification
topic_unstemmed	ddc 540 bkl 42.15 misc Bioinspired nanomaterials misc Lactoferrin misc DNA misc Protein recognition misc Protein-DNA interaction misc Superparamagnetic nanoparticles misc Magnetic purification
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title	Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
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title_full	Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
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author_browse	Cecconello, Alessandro Tonolo, Federica Rilievo, Graziano Molinari, Simone Talpe, Arthur Cozza, Giorgio Venerando, Andrea Kariyawasam, Indipalage Dinusha Harshani Govardhan, Gayathri Tiruchi Arusei, Ruth Jepchirchir Magro, Massimiliano Vianello, Fabio
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title_sort	highly specific colloidal ɣ-fe 2 o 3 -dna hybrids: from bioinspired recognition to large-scale lactoferrin purification
title_auth	Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
abstract	The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility.
abstractGer	The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility.
abstract_unstemmed	The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility.
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title_short	Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
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author2	Tonolo, Federica Rilievo, Graziano Molinari, Simone Talpe, Arthur Cozza, Giorgio Venerando, Andrea Kariyawasam, Indipalage Dinusha Harshani Govardhan, Gayathri Tiruchi Arusei, Ruth Jepchirchir Magro, Massimiliano Vianello, Fabio
author2Str	Tonolo, Federica Rilievo, Graziano Molinari, Simone Talpe, Arthur Cozza, Giorgio Venerando, Andrea Kariyawasam, Indipalage Dinusha Harshani Govardhan, Gayathri Tiruchi Arusei, Ruth Jepchirchir Magro, Massimiliano Vianello, Fabio
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doi_str	10.1016/j.colsurfb.2023.113700
up_date	2024-07-06T19:20:17.239Z
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Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification

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