Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification
The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, sca...
Ausführliche Beschreibung
Autor*in: |
Cecconello, Alessandro [verfasserIn] Tonolo, Federica [verfasserIn] Rilievo, Graziano [verfasserIn] Molinari, Simone [verfasserIn] Talpe, Arthur [verfasserIn] Cozza, Giorgio [verfasserIn] Venerando, Andrea [verfasserIn] Kariyawasam, Indipalage Dinusha Harshani [verfasserIn] Govardhan, Gayathri Tiruchi [verfasserIn] Arusei, Ruth Jepchirchir [verfasserIn] Magro, Massimiliano [verfasserIn] Vianello, Fabio [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Colloids and surfaces / B - Amsterdam [u.a.] : Elsevier Science, 1993, 234 |
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Übergeordnetes Werk: |
volume:234 |
DOI / URN: |
10.1016/j.colsurfb.2023.113700 |
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Katalog-ID: |
ELV066884233 |
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245 | 1 | 0 | |a Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
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520 | |a The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. | ||
650 | 4 | |a Bioinspired nanomaterials | |
650 | 4 | |a Lactoferrin | |
650 | 4 | |a DNA | |
650 | 4 | |a Protein recognition | |
650 | 4 | |a Protein-DNA interaction | |
650 | 4 | |a Superparamagnetic nanoparticles | |
650 | 4 | |a Magnetic purification | |
700 | 1 | |a Tonolo, Federica |e verfasserin |0 (orcid)0000-0002-7780-8994 |4 aut | |
700 | 1 | |a Rilievo, Graziano |e verfasserin |0 (orcid)0000-0003-4098-5636 |4 aut | |
700 | 1 | |a Molinari, Simone |e verfasserin |4 aut | |
700 | 1 | |a Talpe, Arthur |e verfasserin |0 (orcid)0009-0009-5257-9820 |4 aut | |
700 | 1 | |a Cozza, Giorgio |e verfasserin |4 aut | |
700 | 1 | |a Venerando, Andrea |e verfasserin |0 (orcid)0000-0003-0379-2309 |4 aut | |
700 | 1 | |a Kariyawasam, Indipalage Dinusha Harshani |e verfasserin |4 aut | |
700 | 1 | |a Govardhan, Gayathri Tiruchi |e verfasserin |4 aut | |
700 | 1 | |a Arusei, Ruth Jepchirchir |e verfasserin |4 aut | |
700 | 1 | |a Magro, Massimiliano |e verfasserin |0 (orcid)0000-0002-4644-004X |4 aut | |
700 | 1 | |a Vianello, Fabio |e verfasserin |0 (orcid)0000-0002-4874-7205 |4 aut | |
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2023 |
allfields |
10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234 |
spelling |
10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234 |
allfields_unstemmed |
10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234 |
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10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234 |
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10.1016/j.colsurfb.2023.113700 doi (DE-627)ELV066884233 (ELSEVIER)S0927-7765(23)00594-5 DE-627 ger DE-627 rda eng 540 VZ 42.15 bkl Cecconello, Alessandro verfasserin aut Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification Tonolo, Federica verfasserin (orcid)0000-0002-7780-8994 aut Rilievo, Graziano verfasserin (orcid)0000-0003-4098-5636 aut Molinari, Simone verfasserin aut Talpe, Arthur verfasserin (orcid)0009-0009-5257-9820 aut Cozza, Giorgio verfasserin aut Venerando, Andrea verfasserin (orcid)0000-0003-0379-2309 aut Kariyawasam, Indipalage Dinusha Harshani verfasserin aut Govardhan, Gayathri Tiruchi verfasserin aut Arusei, Ruth Jepchirchir verfasserin aut Magro, Massimiliano verfasserin (orcid)0000-0002-4644-004X aut Vianello, Fabio verfasserin (orcid)0000-0002-4874-7205 aut Enthalten in Colloids and surfaces / B Amsterdam [u.a.] : Elsevier Science, 1993 234 Online-Ressource (DE-627)306660016 (DE-600)1500523-9 (DE-576)098614851 1873-4367 nnns volume:234 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2411 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.15 Zellbiologie VZ AR 234 |
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Enthalten in Colloids and surfaces / B 234 volume:234 |
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Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification |
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Cecconello, Alessandro @@aut@@ Tonolo, Federica @@aut@@ Rilievo, Graziano @@aut@@ Molinari, Simone @@aut@@ Talpe, Arthur @@aut@@ Cozza, Giorgio @@aut@@ Venerando, Andrea @@aut@@ Kariyawasam, Indipalage Dinusha Harshani @@aut@@ Govardhan, Gayathri Tiruchi @@aut@@ Arusei, Ruth Jepchirchir @@aut@@ Magro, Massimiliano @@aut@@ Vianello, Fabio @@aut@@ |
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2023-01-01T00:00:00Z |
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Cecconello, Alessandro |
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Cecconello, Alessandro ddc 540 bkl 42.15 misc Bioinspired nanomaterials misc Lactoferrin misc DNA misc Protein recognition misc Protein-DNA interaction misc Superparamagnetic nanoparticles misc Magnetic purification Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
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540 VZ 42.15 bkl Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification Bioinspired nanomaterials Lactoferrin DNA Protein recognition Protein-DNA interaction Superparamagnetic nanoparticles Magnetic purification |
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ddc 540 bkl 42.15 misc Bioinspired nanomaterials misc Lactoferrin misc DNA misc Protein recognition misc Protein-DNA interaction misc Superparamagnetic nanoparticles misc Magnetic purification |
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Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
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Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
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Cecconello, Alessandro Tonolo, Federica Rilievo, Graziano Molinari, Simone Talpe, Arthur Cozza, Giorgio Venerando, Andrea Kariyawasam, Indipalage Dinusha Harshani Govardhan, Gayathri Tiruchi Arusei, Ruth Jepchirchir Magro, Massimiliano Vianello, Fabio |
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highly specific colloidal ɣ-fe 2 o 3 -dna hybrids: from bioinspired recognition to large-scale lactoferrin purification |
title_auth |
Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
abstract |
The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. |
abstractGer |
The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. |
abstract_unstemmed |
The industry transfer of laboratory-use magnetic separation is still hampered by the lack of suitable nanoparticles, both in terms of their features and large-scale availability. Surface Active Maghemite Nanoparticles (SAMNs) characterized by a unique surface chemistry, low environmental impact, scalable synthesis and functionalization were used to develop a bio-inspired lactoferrin (LF) recognition system. Based on the LF affinity for DNA, a self-assembly process was optimized for obtaining a SAMNDNA hybrid displaying chemical and colloidal stability and LF specificity. SAMN@DNA was successfully tested for the affinity purification of LF from crude bovine whey. Advantages, such as high selectivity and loading capacity, nanoparticle re-usability, outstanding purity (96 ± 1%), preservation of protein conformation and short operational time, were highlighted. Finally, scalability was demonstrated by an automatic system performing continuous purification of LF from 100 liters day−1 of whey. This study responds to essential prerequisites, such as efficiency, re-usability and industrialization feasibility. |
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title_short |
Highly specific colloidal ɣ-Fe 2 O 3 -DNA hybrids: From bioinspired recognition to large-scale lactoferrin purification |
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Tonolo, Federica Rilievo, Graziano Molinari, Simone Talpe, Arthur Cozza, Giorgio Venerando, Andrea Kariyawasam, Indipalage Dinusha Harshani Govardhan, Gayathri Tiruchi Arusei, Ruth Jepchirchir Magro, Massimiliano Vianello, Fabio |
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score |
7.400301 |