Electrophoretic separations using sweeping fields
Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that fol...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1998 |
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| Umfang: |
7 Ill. 6 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
|---|---|
| Übergeordnetes Werk: |
in: Electrophoresis - Weinheim : Wiley-VCH, 19(1998), Seite 1388-1393 |
| Übergeordnetes Werk: |
volume:19 ; year:1998 ; month:8- ; pages:1388-1393 ; extent:6 |
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| 520 | |a Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. | ||
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| 700 | 1 | |a Mastrangelo, Carlos H. |4 oth | |
| 700 | 1 | |a Burke, David T. |4 oth | |
| 700 | 1 | |a Burns, Mark A. |4 oth | |
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(DE-627)NLEJ15937247X DE-627 ger DE-627 rakwb eng Electrophoretic separations using sweeping fields 1998 7 Ill. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. Wiley InterScience Backfile Collection 1832-2000 Ying, Feng oth Mastrangelo, Carlos H. oth Burke, David T. oth Burns, Mark A. oth in Electrophoresis Weinheim : Wiley-VCH 19(1998), Seite 1388-1393 (DE-627)NLEJ15907052X (DE-600)1475486-1 0173-0835 nnns volume:19 year:1998 month:8- pages:1388-1393 extent:6 http://dx.doi.org/10.1002/elps.1150190832 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 19 1998 8-9 1388-1393 6 |
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(DE-627)NLEJ15937247X DE-627 ger DE-627 rakwb eng Electrophoretic separations using sweeping fields 1998 7 Ill. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. Wiley InterScience Backfile Collection 1832-2000 Ying, Feng oth Mastrangelo, Carlos H. oth Burke, David T. oth Burns, Mark A. oth in Electrophoresis Weinheim : Wiley-VCH 19(1998), Seite 1388-1393 (DE-627)NLEJ15907052X (DE-600)1475486-1 0173-0835 nnns volume:19 year:1998 month:8- pages:1388-1393 extent:6 http://dx.doi.org/10.1002/elps.1150190832 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 19 1998 8-9 1388-1393 6 |
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(DE-627)NLEJ15937247X DE-627 ger DE-627 rakwb eng Electrophoretic separations using sweeping fields 1998 7 Ill. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. Wiley InterScience Backfile Collection 1832-2000 Ying, Feng oth Mastrangelo, Carlos H. oth Burke, David T. oth Burns, Mark A. oth in Electrophoresis Weinheim : Wiley-VCH 19(1998), Seite 1388-1393 (DE-627)NLEJ15907052X (DE-600)1475486-1 0173-0835 nnns volume:19 year:1998 month:8- pages:1388-1393 extent:6 http://dx.doi.org/10.1002/elps.1150190832 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 19 1998 8-9 1388-1393 6 |
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(DE-627)NLEJ15937247X DE-627 ger DE-627 rakwb eng Electrophoretic separations using sweeping fields 1998 7 Ill. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. Wiley InterScience Backfile Collection 1832-2000 Ying, Feng oth Mastrangelo, Carlos H. oth Burke, David T. oth Burns, Mark A. oth in Electrophoresis Weinheim : Wiley-VCH 19(1998), Seite 1388-1393 (DE-627)NLEJ15907052X (DE-600)1475486-1 0173-0835 nnns volume:19 year:1998 month:8- pages:1388-1393 extent:6 http://dx.doi.org/10.1002/elps.1150190832 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 19 1998 8-9 1388-1393 6 |
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(DE-627)NLEJ15937247X DE-627 ger DE-627 rakwb eng Electrophoretic separations using sweeping fields 1998 7 Ill. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. Wiley InterScience Backfile Collection 1832-2000 Ying, Feng oth Mastrangelo, Carlos H. oth Burke, David T. oth Burns, Mark A. oth in Electrophoresis Weinheim : Wiley-VCH 19(1998), Seite 1388-1393 (DE-627)NLEJ15907052X (DE-600)1475486-1 0173-0835 nnns volume:19 year:1998 month:8- pages:1388-1393 extent:6 http://dx.doi.org/10.1002/elps.1150190832 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 19 1998 8-9 1388-1393 6 |
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Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. |
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Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. |
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Sweeping-field electrophoresis is investigated as a method for increasing the resolution of low-voltage slab gel separations. In this technique a low direct current (DC) voltage is time multiplexed to an array of periodically spaced electrodes placed along the length of the slab in a manner that follows the band migration. Because the electrode spacing is smaller than the slab length, a larger field is generated, yielding an improved separation. The effect of the nonuniform electric field on band distortion is studied in some detail. Experimental band distortion results showed good agreement with theoretical predictions in a macroscopic sweeping-field electrophoresis system. Both analytical and numerical results show that band distortion can be effectively minimized when an appropriate sweep rate is selected for a narrow band range. Using this scheme we have achieved the same number of theoretical plates as a DC-driven system with one third of the drive voltage. |
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