Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.

Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated...
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Gespeichert in:

Autor*in:	Zimmer, Ch. Venner, H.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	1966

Umfang:	2 Ill. ; 2 Tab. 7

Reproduktion:	Wiley InterScience Backfile Collection 1832-2000
Übergeordnetes Werk:	in: Biopolymers - New York : John Wiley & Sons, Inc., 4(1966) vom: Okt., Seite 1073-1079
Übergeordnetes Werk:	volume:4 ; year:1966 ; month:10 ; pages:1073-1079 ; extent:7

Links:	Link aufrufen

Katalog-ID:	NLEJ159537320

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520			\|a Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration.
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allfields	(DE-627)NLEJ159537320 DE-627 ger DE-627 rakwb eng Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965. 1966 2 Ill. 2 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration. Wiley InterScience Backfile Collection 1832-2000 Zimmer, Ch. oth Venner, H. oth in Biopolymers New York : John Wiley & Sons, Inc. 4(1966) vom: Okt., Seite 1073-1079 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:4 year:1966 month:10 pages:1073-1079 extent:7 http://dx.doi.org/10.1002/bip.1966.360041004 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 4 1966 10 1073-1079 7
spelling	(DE-627)NLEJ159537320 DE-627 ger DE-627 rakwb eng Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965. 1966 2 Ill. 2 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration. Wiley InterScience Backfile Collection 1832-2000 Zimmer, Ch. oth Venner, H. oth in Biopolymers New York : John Wiley & Sons, Inc. 4(1966) vom: Okt., Seite 1073-1079 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:4 year:1966 month:10 pages:1073-1079 extent:7 http://dx.doi.org/10.1002/bip.1966.360041004 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 4 1966 10 1073-1079 7
allfields_unstemmed	(DE-627)NLEJ159537320 DE-627 ger DE-627 rakwb eng Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965. 1966 2 Ill. 2 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration. Wiley InterScience Backfile Collection 1832-2000 Zimmer, Ch. oth Venner, H. oth in Biopolymers New York : John Wiley & Sons, Inc. 4(1966) vom: Okt., Seite 1073-1079 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:4 year:1966 month:10 pages:1073-1079 extent:7 http://dx.doi.org/10.1002/bip.1966.360041004 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 4 1966 10 1073-1079 7
allfieldsGer	(DE-627)NLEJ159537320 DE-627 ger DE-627 rakwb eng Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965. 1966 2 Ill. 2 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration. Wiley InterScience Backfile Collection 1832-2000 Zimmer, Ch. oth Venner, H. oth in Biopolymers New York : John Wiley & Sons, Inc. 4(1966) vom: Okt., Seite 1073-1079 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:4 year:1966 month:10 pages:1073-1079 extent:7 http://dx.doi.org/10.1002/bip.1966.360041004 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 4 1966 10 1073-1079 7
allfieldsSound	(DE-627)NLEJ159537320 DE-627 ger DE-627 rakwb eng Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965. 1966 2 Ill. 2 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration. Wiley InterScience Backfile Collection 1832-2000 Zimmer, Ch. oth Venner, H. oth in Biopolymers New York : John Wiley & Sons, Inc. 4(1966) vom: Okt., Seite 1073-1079 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:4 year:1966 month:10 pages:1073-1079 extent:7 http://dx.doi.org/10.1002/bip.1966.360041004 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 4 1966 10 1073-1079 7
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topic_title	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965
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title	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.
spellingShingle	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.
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title_full	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965
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title_sort	protonation of cytosine in dnapresented in part at the 3rd jena symposium, elektrochemische prinzipien in der molekularbiologie, jena, may 25-29, 1965
title_auth	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.
abstract	Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration.
abstractGer	Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration.
abstract_unstemmed	Spectrophotometric acid titrations of DNA samples of different GC content were performed at different wavelengths. From the acid titration profile and absorbance changes of deoxycytidine-5′-monophosphate and DNA the extent of protonated cytosine within the DNA double-stranded molecule was estimated (pK 3.65 at 25°C. in 0.02M KCl). At constant counterion concentration and temperature the maximum of protonated cytosine in DNA before denaturation occurs depends on the base composition and can exceed 50%. The thermal stability of the DNA secondary structure is strongly reduced with increasing amount of ionized cytosine residues. The degree of protonation of cytosine in DNA is decreased with increasing counterion concentration.
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title_short	Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.
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Protonation of cytosine in DNAPresented in part at the 3rd Jena Symposium, Elektrochemische Prinzipien in der Molekularbiologie, Jena, May 25-29, 1965.

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