Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes
The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in th...
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1970 |
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| Umfang: |
9 Ill. ; 3 Tab. 22 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Biopolymers - New York : John Wiley & Sons, Inc., 9(1970) vom: Juli, Seite 843-864 |
| Übergeordnetes Werk: |
volume:9 ; year:1970 ; month:07 ; pages:843-864 ; extent:22 |
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| 520 | |a The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. | ||
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(DE-627)NLEJ159542537 DE-627 ger DE-627 rakwb eng Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes 1970 9 Ill. 3 Tab. 22 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. Wiley InterScience Backfile Collection 1832-2000 Rodgers, A. oth in Biopolymers New York : John Wiley & Sons, Inc. 9(1970) vom: Juli, Seite 843-864 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:9 year:1970 month:07 pages:843-864 extent:22 http://dx.doi.org/10.1002/bip.1970.360090709 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 9 1970 7 843-864 22 |
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(DE-627)NLEJ159542537 DE-627 ger DE-627 rakwb eng Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes 1970 9 Ill. 3 Tab. 22 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. Wiley InterScience Backfile Collection 1832-2000 Rodgers, A. oth in Biopolymers New York : John Wiley & Sons, Inc. 9(1970) vom: Juli, Seite 843-864 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:9 year:1970 month:07 pages:843-864 extent:22 http://dx.doi.org/10.1002/bip.1970.360090709 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 9 1970 7 843-864 22 |
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(DE-627)NLEJ159542537 DE-627 ger DE-627 rakwb eng Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes 1970 9 Ill. 3 Tab. 22 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. Wiley InterScience Backfile Collection 1832-2000 Rodgers, A. oth in Biopolymers New York : John Wiley & Sons, Inc. 9(1970) vom: Juli, Seite 843-864 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:9 year:1970 month:07 pages:843-864 extent:22 http://dx.doi.org/10.1002/bip.1970.360090709 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 9 1970 7 843-864 22 |
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(DE-627)NLEJ159542537 DE-627 ger DE-627 rakwb eng Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes 1970 9 Ill. 3 Tab. 22 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. Wiley InterScience Backfile Collection 1832-2000 Rodgers, A. oth in Biopolymers New York : John Wiley & Sons, Inc. 9(1970) vom: Juli, Seite 843-864 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:9 year:1970 month:07 pages:843-864 extent:22 http://dx.doi.org/10.1002/bip.1970.360090709 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 9 1970 7 843-864 22 |
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(DE-627)NLEJ159542537 DE-627 ger DE-627 rakwb eng Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes 1970 9 Ill. 3 Tab. 22 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. Wiley InterScience Backfile Collection 1832-2000 Rodgers, A. oth in Biopolymers New York : John Wiley & Sons, Inc. 9(1970) vom: Juli, Seite 843-864 (DE-627)NLEJ15907066X (DE-600)1480801-8 0006-3525 nnns volume:9 year:1970 month:07 pages:843-864 extent:22 http://dx.doi.org/10.1002/bip.1970.360090709 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 9 1970 7 843-864 22 |
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sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes |
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Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes |
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The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. |
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The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. |
| abstract_unstemmed |
The formation of a slowly sedimenting form of 23-S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23-S arises as a result of nucleate damage to the RNA in the 50-S ribosome. The 30-S ribosome (and 16-S RNA), is unaffected. The slow form of 23-S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01M Na). Apparent maintenance of the integrity of 23-S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure. |
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