A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose
A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Umfang: |
6 Ill. ; 1 Tab. 6 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Biotechnology and Bioengineering - New York, NY [u.a.] : Wiley, 34(1989) vom: Aug., Seite 1098-1103 |
| Übergeordnetes Werk: |
volume:34 ; year:1989 ; month:08 ; pages:1098-1103 ; extent:6 |
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| 520 | |a A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. | ||
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(DE-627)NLEJ15964450X DE-627 ger DE-627 rakwb eng A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose 1989 6 Ill. 1 Tab. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. Wiley InterScience Backfile Collection 1832-2000 Sakoda, Akiyoshi oth Wang, Henry Y. oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 34(1989) vom: Aug., Seite 1098-1103 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:34 year:1989 month:08 pages:1098-1103 extent:6 http://dx.doi.org/10.1002/bit.260340811 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 34 1989 8 1098-1103 6 |
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(DE-627)NLEJ15964450X DE-627 ger DE-627 rakwb eng A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose 1989 6 Ill. 1 Tab. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. Wiley InterScience Backfile Collection 1832-2000 Sakoda, Akiyoshi oth Wang, Henry Y. oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 34(1989) vom: Aug., Seite 1098-1103 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:34 year:1989 month:08 pages:1098-1103 extent:6 http://dx.doi.org/10.1002/bit.260340811 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 34 1989 8 1098-1103 6 |
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(DE-627)NLEJ15964450X DE-627 ger DE-627 rakwb eng A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose 1989 6 Ill. 1 Tab. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. Wiley InterScience Backfile Collection 1832-2000 Sakoda, Akiyoshi oth Wang, Henry Y. oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 34(1989) vom: Aug., Seite 1098-1103 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:34 year:1989 month:08 pages:1098-1103 extent:6 http://dx.doi.org/10.1002/bit.260340811 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 34 1989 8 1098-1103 6 |
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(DE-627)NLEJ15964450X DE-627 ger DE-627 rakwb eng A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose 1989 6 Ill. 1 Tab. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. Wiley InterScience Backfile Collection 1832-2000 Sakoda, Akiyoshi oth Wang, Henry Y. oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 34(1989) vom: Aug., Seite 1098-1103 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:34 year:1989 month:08 pages:1098-1103 extent:6 http://dx.doi.org/10.1002/bit.260340811 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 34 1989 8 1098-1103 6 |
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(DE-627)NLEJ15964450X DE-627 ger DE-627 rakwb eng A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose 1989 6 Ill. 1 Tab. 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. Wiley InterScience Backfile Collection 1832-2000 Sakoda, Akiyoshi oth Wang, Henry Y. oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 34(1989) vom: Aug., Seite 1098-1103 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:34 year:1989 month:08 pages:1098-1103 extent:6 http://dx.doi.org/10.1002/bit.260340811 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 34 1989 8 1098-1103 6 |
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A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose |
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A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. |
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A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. |
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A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods. |
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