Growth kinetics of a bacteriophage in continuous culture
Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1996 |
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| Umfang: |
3 Ill. 5 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Biotechnology and Bioengineering - New York, NY [u.a.] : Wiley, 50(1996) vom: Feb., Seite 217-221 |
| Übergeordnetes Werk: |
volume:50 ; year:1996 ; month:02 ; pages:217-221 ; extent:5 |
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| 520 | |a Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. | ||
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(DE-627)NLEJ159665817 DE-627 ger DE-627 rakwb eng Growth kinetics of a bacteriophage in continuous culture 1996 3 Ill. 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. Wiley InterScience Backfile Collection 1832-2000 Schwienhorst, Andreas oth Lindemann, Björn F. oth Eigen, Manfred oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 50(1996) vom: Feb., Seite 217-221 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:50 year:1996 month:02 pages:217-221 extent:5 http://dx.doi.org/10.1002/bit.260500204 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 50 1996 2 217-221 5 |
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(DE-627)NLEJ159665817 DE-627 ger DE-627 rakwb eng Growth kinetics of a bacteriophage in continuous culture 1996 3 Ill. 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. Wiley InterScience Backfile Collection 1832-2000 Schwienhorst, Andreas oth Lindemann, Björn F. oth Eigen, Manfred oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 50(1996) vom: Feb., Seite 217-221 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:50 year:1996 month:02 pages:217-221 extent:5 http://dx.doi.org/10.1002/bit.260500204 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 50 1996 2 217-221 5 |
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(DE-627)NLEJ159665817 DE-627 ger DE-627 rakwb eng Growth kinetics of a bacteriophage in continuous culture 1996 3 Ill. 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. Wiley InterScience Backfile Collection 1832-2000 Schwienhorst, Andreas oth Lindemann, Björn F. oth Eigen, Manfred oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 50(1996) vom: Feb., Seite 217-221 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:50 year:1996 month:02 pages:217-221 extent:5 http://dx.doi.org/10.1002/bit.260500204 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 50 1996 2 217-221 5 |
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(DE-627)NLEJ159665817 DE-627 ger DE-627 rakwb eng Growth kinetics of a bacteriophage in continuous culture 1996 3 Ill. 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. Wiley InterScience Backfile Collection 1832-2000 Schwienhorst, Andreas oth Lindemann, Björn F. oth Eigen, Manfred oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 50(1996) vom: Feb., Seite 217-221 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:50 year:1996 month:02 pages:217-221 extent:5 http://dx.doi.org/10.1002/bit.260500204 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 50 1996 2 217-221 5 |
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(DE-627)NLEJ159665817 DE-627 ger DE-627 rakwb eng Growth kinetics of a bacteriophage in continuous culture 1996 3 Ill. 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. Wiley InterScience Backfile Collection 1832-2000 Schwienhorst, Andreas oth Lindemann, Björn F. oth Eigen, Manfred oth in Biotechnology and Bioengineering New York, NY [u.a.] : Wiley 50(1996) vom: Feb., Seite 217-221 (DE-627)NLEJ159070678 (DE-600)1480809-2 0006-3592 nnns volume:50 year:1996 month:02 pages:217-221 extent:5 http://dx.doi.org/10.1002/bit.260500204 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 50 1996 2 217-221 5 |
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Growth kinetics of a bacteriophage in continuous culture |
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Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. |
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Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. |
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Lytic coliphage Qβ was grown in continuously cultured host bacteria using a cascade of stirred flow reactors. The apparatus was constructed so that the steady stream of exponentially growing bacterial cells passing through the stirred flow reactors served to prevent coevolution brought about by host-parasite interactions. Wall growth was the primary cause for deviation from ideal continuous culture conditions and is largely dependent on the surface structure of the host bacteria. Using an Escherichia coli strain deficient in adhesive type I pili expression, the desynchronization of single burst events could easily be followed over the course of four infection latency periods. Computer simulations based on a two-stage model for the Qβ infection cycle were in perfect agreement with the experimental data. Applications of the optimized system to strategies of molecular evolution are discussed. © 1996 John Wiley & Sons, Inc. |
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