Rates of protein synthesis by hepatocytes isolated from rats of various ages
The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1978 |
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| Umfang: |
4 Ill. ; 4 Tab. 10 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
|---|---|
| Übergeordnetes Werk: |
in: Journal of Cellular Physiology - New York, NY [u.a.] : Wiley-Liss, 97(1978) vom: Feb., Seite 137-146 |
| Übergeordnetes Werk: |
volume:97 ; year:1978 ; month:02 ; pages:137-146 ; extent:10 |
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| 520 | |a The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. | ||
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| 700 | 1 | |a Richardson, Arlan |4 oth | |
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(DE-627)NLEJ160771307 DE-627 ger DE-627 rakwb eng Rates of protein synthesis by hepatocytes isolated from rats of various ages 1978 4 Ill. 4 Tab. 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. Wiley InterScience Backfile Collection 1832-2000 Ricca, George A. oth Liu, Daniel S. H. oth Coniglio, John J. oth Richardson, Arlan oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 97(1978) vom: Feb., Seite 137-146 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:97 year:1978 month:02 pages:137-146 extent:10 http://dx.doi.org/10.1002/jcp.1040970202 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 97 1978 2 137-146 10 |
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(DE-627)NLEJ160771307 DE-627 ger DE-627 rakwb eng Rates of protein synthesis by hepatocytes isolated from rats of various ages 1978 4 Ill. 4 Tab. 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. Wiley InterScience Backfile Collection 1832-2000 Ricca, George A. oth Liu, Daniel S. H. oth Coniglio, John J. oth Richardson, Arlan oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 97(1978) vom: Feb., Seite 137-146 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:97 year:1978 month:02 pages:137-146 extent:10 http://dx.doi.org/10.1002/jcp.1040970202 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 97 1978 2 137-146 10 |
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(DE-627)NLEJ160771307 DE-627 ger DE-627 rakwb eng Rates of protein synthesis by hepatocytes isolated from rats of various ages 1978 4 Ill. 4 Tab. 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. Wiley InterScience Backfile Collection 1832-2000 Ricca, George A. oth Liu, Daniel S. H. oth Coniglio, John J. oth Richardson, Arlan oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 97(1978) vom: Feb., Seite 137-146 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:97 year:1978 month:02 pages:137-146 extent:10 http://dx.doi.org/10.1002/jcp.1040970202 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 97 1978 2 137-146 10 |
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(DE-627)NLEJ160771307 DE-627 ger DE-627 rakwb eng Rates of protein synthesis by hepatocytes isolated from rats of various ages 1978 4 Ill. 4 Tab. 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. Wiley InterScience Backfile Collection 1832-2000 Ricca, George A. oth Liu, Daniel S. H. oth Coniglio, John J. oth Richardson, Arlan oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 97(1978) vom: Feb., Seite 137-146 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:97 year:1978 month:02 pages:137-146 extent:10 http://dx.doi.org/10.1002/jcp.1040970202 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 97 1978 2 137-146 10 |
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(DE-627)NLEJ160771307 DE-627 ger DE-627 rakwb eng Rates of protein synthesis by hepatocytes isolated from rats of various ages 1978 4 Ill. 4 Tab. 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. Wiley InterScience Backfile Collection 1832-2000 Ricca, George A. oth Liu, Daniel S. H. oth Coniglio, John J. oth Richardson, Arlan oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 97(1978) vom: Feb., Seite 137-146 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:97 year:1978 month:02 pages:137-146 extent:10 http://dx.doi.org/10.1002/jcp.1040970202 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 97 1978 2 137-146 10 |
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Rates of protein synthesis by hepatocytes isolated from rats of various ages |
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The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. |
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The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. |
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The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ160771307</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707044221.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070201s1978 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ160771307</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Rates of protein synthesis by hepatocytes isolated from rats of various ages</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1978</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="b">4 Ill.</subfield><subfield code="b">4 Tab.</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">10</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6 mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Wiley InterScience Backfile Collection 1832-2000</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ricca, George A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Daniel S. H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Coniglio, John J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Richardson, Arlan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Journal of Cellular Physiology</subfield><subfield code="d">New York, NY [u.a.] : Wiley-Liss</subfield><subfield code="g">97(1978) vom: Feb., Seite 137-146</subfield><subfield code="w">(DE-627)NLEJ15907097X</subfield><subfield code="w">(DE-600)1478143-8</subfield><subfield code="x">0021-9541</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:97</subfield><subfield code="g">year:1978</subfield><subfield code="g">month:02</subfield><subfield code="g">pages:137-146</subfield><subfield code="g">extent:10</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1002/jcp.1040970202</subfield><subfield code="q">text/html</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-WIS</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">97</subfield><subfield code="j">1978</subfield><subfield code="c">2</subfield><subfield code="h">137-146</subfield><subfield code="g">10</subfield></datafield></record></collection>
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