Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors
A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endot...
Ausführliche Beschreibung
Gespeichert in:
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1981 |
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| Umfang: |
6 Ill. ; 6 Tab. 12 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Journal of Cellular Physiology - New York, NY [u.a.] : Wiley-Liss, 109(1981) vom: Feb., Seite 253-264 |
| Übergeordnetes Werk: |
volume:109 ; year:1981 ; month:02 ; pages:253-264 ; extent:12 |
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| 520 | |a A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. | ||
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(DE-627)NLEJ160777941 DE-627 ger DE-627 rakwb eng Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors 1981 6 Ill. 6 Tab. 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. Wiley InterScience Backfile Collection 1832-2000 Nicola, Nicos A. oth Metcalf, Donald oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 109(1981) vom: Feb., Seite 253-264 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:109 year:1981 month:02 pages:253-264 extent:12 http://dx.doi.org/10.1002/jcp.1041090208 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 109 1981 2 253-264 12 |
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(DE-627)NLEJ160777941 DE-627 ger DE-627 rakwb eng Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors 1981 6 Ill. 6 Tab. 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. Wiley InterScience Backfile Collection 1832-2000 Nicola, Nicos A. oth Metcalf, Donald oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 109(1981) vom: Feb., Seite 253-264 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:109 year:1981 month:02 pages:253-264 extent:12 http://dx.doi.org/10.1002/jcp.1041090208 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 109 1981 2 253-264 12 |
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(DE-627)NLEJ160777941 DE-627 ger DE-627 rakwb eng Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors 1981 6 Ill. 6 Tab. 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. Wiley InterScience Backfile Collection 1832-2000 Nicola, Nicos A. oth Metcalf, Donald oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 109(1981) vom: Feb., Seite 253-264 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:109 year:1981 month:02 pages:253-264 extent:12 http://dx.doi.org/10.1002/jcp.1041090208 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 109 1981 2 253-264 12 |
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(DE-627)NLEJ160777941 DE-627 ger DE-627 rakwb eng Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors 1981 6 Ill. 6 Tab. 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. Wiley InterScience Backfile Collection 1832-2000 Nicola, Nicos A. oth Metcalf, Donald oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 109(1981) vom: Feb., Seite 253-264 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:109 year:1981 month:02 pages:253-264 extent:12 http://dx.doi.org/10.1002/jcp.1041090208 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 109 1981 2 253-264 12 |
| allfieldsSound |
(DE-627)NLEJ160777941 DE-627 ger DE-627 rakwb eng Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors 1981 6 Ill. 6 Tab. 12 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. Wiley InterScience Backfile Collection 1832-2000 Nicola, Nicos A. oth Metcalf, Donald oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 109(1981) vom: Feb., Seite 253-264 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:109 year:1981 month:02 pages:253-264 extent:12 http://dx.doi.org/10.1002/jcp.1041090208 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 109 1981 2 253-264 12 |
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biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors |
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Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors |
| abstract |
A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. |
| abstractGer |
A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. |
| abstract_unstemmed |
A number of different organs and tissues from untreated or endotoxin-injected mice were surveyed for their ability to produce conditioned medium that could induce differentiation of the myelomonocytic leukemic cell line WEHI-3B. All organs were able to do this but were much more effective from endotoxin-injected animals. Production of the differentiation factor (DF) was dependent on protein synthesis and occurred throughout the 7-day incubation period. DF produced by the different organs in vitro and found in vivo in endotoxin serum were indistinguishable from each other by several fractionation procedures but could be distinguished from the majority of granulocyte-macrophage and macrophage colony-stimulating factors (GM- and M-CSFs). DF was precipitated by 55% ammonium sulfate whereas CSF required 85% saturation. DF did not bind to Concanavalin A-Sepharose whereas CSF did. DF eluted later than CSF from Phenyl-Sepharose columns. The dissociated molecular weight of DF was 21,000-29,000 and was not greatly affected by neuraminidase treatment. There was no evidence that DF was glycoprotein in nature although this cannot yet be ruled out. Although the DF activity could be clearly separated from the bulk of GM-CSF and M-CSF activities, there was always residual CSF activity associated with the DF. This CSF was distinct from GM- and M-CSF in its biochemical properties, in the fact that it only stimulated a subset of colony-forming cells and that upon dilution it stimulated exclusively granulocytic colonies to develop. In two steps (ammonium sulfate precipitation and Phenyl-Sepharose chromatography) it was possible to selectively enrich the differentiation activity over the CSF activity by 21-fold. |
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Biochemical properties of differentiation factors for murine myelomonocytic leukemic cells in organ conditioned media - separation from colony-stimulating factors |
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Nicola, Nicos A. Metcalf, Donald |
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