Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells
Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential...
Ausführliche Beschreibung
Gespeichert in:
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1984 |
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| Umfang: |
3 Ill. ; 6 Tab. 7 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Journal of Cellular Physiology - New York, NY [u.a.] : Wiley-Liss, 121(1984) vom: Jan., Seite 74-80 |
| Übergeordnetes Werk: |
volume:121 ; year:1984 ; month:01 ; pages:74-80 ; extent:7 |
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| 520 | |a Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. | ||
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(DE-627)NLEJ160784891 DE-627 ger DE-627 rakwb eng Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells 1984 3 Ill. 6 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. Wiley InterScience Backfile Collection 1832-2000 Sato, Seiji oth Yamamoto, Hiroaki oth Yonezawa, Minoru oth Takeuchi, Takuji oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 121(1984) vom: Jan., Seite 74-80 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:121 year:1984 month:01 pages:74-80 extent:7 http://dx.doi.org/10.1002/jcp.1041210110 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 121 1984 1 74-80 7 |
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(DE-627)NLEJ160784891 DE-627 ger DE-627 rakwb eng Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells 1984 3 Ill. 6 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. Wiley InterScience Backfile Collection 1832-2000 Sato, Seiji oth Yamamoto, Hiroaki oth Yonezawa, Minoru oth Takeuchi, Takuji oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 121(1984) vom: Jan., Seite 74-80 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:121 year:1984 month:01 pages:74-80 extent:7 http://dx.doi.org/10.1002/jcp.1041210110 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 121 1984 1 74-80 7 |
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(DE-627)NLEJ160784891 DE-627 ger DE-627 rakwb eng Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells 1984 3 Ill. 6 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. Wiley InterScience Backfile Collection 1832-2000 Sato, Seiji oth Yamamoto, Hiroaki oth Yonezawa, Minoru oth Takeuchi, Takuji oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 121(1984) vom: Jan., Seite 74-80 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:121 year:1984 month:01 pages:74-80 extent:7 http://dx.doi.org/10.1002/jcp.1041210110 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 121 1984 1 74-80 7 |
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(DE-627)NLEJ160784891 DE-627 ger DE-627 rakwb eng Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells 1984 3 Ill. 6 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. Wiley InterScience Backfile Collection 1832-2000 Sato, Seiji oth Yamamoto, Hiroaki oth Yonezawa, Minoru oth Takeuchi, Takuji oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 121(1984) vom: Jan., Seite 74-80 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:121 year:1984 month:01 pages:74-80 extent:7 http://dx.doi.org/10.1002/jcp.1041210110 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 121 1984 1 74-80 7 |
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(DE-627)NLEJ160784891 DE-627 ger DE-627 rakwb eng Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells 1984 3 Ill. 6 Tab. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. Wiley InterScience Backfile Collection 1832-2000 Sato, Seiji oth Yamamoto, Hiroaki oth Yonezawa, Minoru oth Takeuchi, Takuji oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 121(1984) vom: Jan., Seite 74-80 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:121 year:1984 month:01 pages:74-80 extent:7 http://dx.doi.org/10.1002/jcp.1041210110 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 121 1984 1 74-80 7 |
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serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in b16-conv melanoma cells |
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Serum and substratum-dependent coupled loss of differentiated and tumorigenic phenotypes in B16-conv melanoma cells |
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Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. |
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Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. |
| abstract_unstemmed |
Clonal B16 mouse melanoma conv cells are tumorigenic spindle-shaped cells (S-type cells) exhibiting tyrosinase activity and melanosomes under usual culture conditions. When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating. |
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When the cells passaged on glass substratum were plated for colony formation on plastic substratum in Eagle's minimum essential medium (MEM) with 10% calf bovine serum, most of them converted to fibroblastlike cells (F-type cells) with the coupled loss of differentiated and tumorigenic phenotypes. However, they continued to be S-type cells provided that they were plated on glass substratum. The conversion from S- to F-type cell was not induced with high frequency even on plastic substratum when the concentration of calf serum in the medium was low (1-2%). These results indicate that both plastic substratum and serum factor are requisites for converting the phenotypic expression of the conv cells. Partial characterization of the serum factor indicates that it is adsorbable to plastic substratum, inactivated at 70°C for 10 min, salted out at 40% of saturated ammonium sulfate; in addition the factor seems to act on cells within 1 day after plating.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Wiley InterScience Backfile Collection 1832-2000</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sato, Seiji</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yamamoto, Hiroaki</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yonezawa, Minoru</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Takeuchi, Takuji</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Journal of Cellular Physiology</subfield><subfield code="d">New York, NY [u.a.] : Wiley-Liss</subfield><subfield code="g">121(1984) vom: Jan., Seite 74-80</subfield><subfield code="w">(DE-627)NLEJ15907097X</subfield><subfield code="w">(DE-600)1478143-8</subfield><subfield code="x">0021-9541</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:121</subfield><subfield code="g">year:1984</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:74-80</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1002/jcp.1041210110</subfield><subfield code="q">text/html</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-WIS</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">121</subfield><subfield code="j">1984</subfield><subfield code="c">1</subfield><subfield code="h">74-80</subfield><subfield code="g">7</subfield></datafield></record></collection>
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