Cell volume regulation of cerebrovascular endothelium in vitro
Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. En...
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Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1985 |
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| Umfang: |
1 Ill. ; 1 Tab. 4 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Journal of Cellular Physiology - New York, NY [u.a.] : Wiley-Liss, 123(1985) vom: Jan., Seite 51-54 |
| Übergeordnetes Werk: |
volume:123 ; year:1985 ; month:01 ; pages:51-54 ; extent:4 |
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NLEJ160786509 |
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| 520 | |a Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. | ||
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(DE-627)NLEJ160786509 DE-627 ger DE-627 rakwb eng Cell volume regulation of cerebrovascular endothelium in vitro 1985 1 Ill. 1 Tab. 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. Wiley InterScience Backfile Collection 1832-2000 Kempski, O. oth Spatz, M. oth Valet, G. oth Baethmann, A. oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 123(1985) vom: Jan., Seite 51-54 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:123 year:1985 month:01 pages:51-54 extent:4 http://dx.doi.org/10.1002/jcp.1041230109 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 123 1985 1 51-54 4 |
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(DE-627)NLEJ160786509 DE-627 ger DE-627 rakwb eng Cell volume regulation of cerebrovascular endothelium in vitro 1985 1 Ill. 1 Tab. 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. Wiley InterScience Backfile Collection 1832-2000 Kempski, O. oth Spatz, M. oth Valet, G. oth Baethmann, A. oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 123(1985) vom: Jan., Seite 51-54 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:123 year:1985 month:01 pages:51-54 extent:4 http://dx.doi.org/10.1002/jcp.1041230109 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 123 1985 1 51-54 4 |
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(DE-627)NLEJ160786509 DE-627 ger DE-627 rakwb eng Cell volume regulation of cerebrovascular endothelium in vitro 1985 1 Ill. 1 Tab. 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. Wiley InterScience Backfile Collection 1832-2000 Kempski, O. oth Spatz, M. oth Valet, G. oth Baethmann, A. oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 123(1985) vom: Jan., Seite 51-54 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:123 year:1985 month:01 pages:51-54 extent:4 http://dx.doi.org/10.1002/jcp.1041230109 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 123 1985 1 51-54 4 |
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(DE-627)NLEJ160786509 DE-627 ger DE-627 rakwb eng Cell volume regulation of cerebrovascular endothelium in vitro 1985 1 Ill. 1 Tab. 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. Wiley InterScience Backfile Collection 1832-2000 Kempski, O. oth Spatz, M. oth Valet, G. oth Baethmann, A. oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 123(1985) vom: Jan., Seite 51-54 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:123 year:1985 month:01 pages:51-54 extent:4 http://dx.doi.org/10.1002/jcp.1041230109 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 123 1985 1 51-54 4 |
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(DE-627)NLEJ160786509 DE-627 ger DE-627 rakwb eng Cell volume regulation of cerebrovascular endothelium in vitro 1985 1 Ill. 1 Tab. 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. Wiley InterScience Backfile Collection 1832-2000 Kempski, O. oth Spatz, M. oth Valet, G. oth Baethmann, A. oth in Journal of Cellular Physiology New York, NY [u.a.] : Wiley-Liss 123(1985) vom: Jan., Seite 51-54 (DE-627)NLEJ15907097X (DE-600)1478143-8 0021-9541 nnns volume:123 year:1985 month:01 pages:51-54 extent:4 http://dx.doi.org/10.1002/jcp.1041230109 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 123 1985 1 51-54 4 |
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Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. |
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Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. |
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Regulation of cell volume as a fundamental cellular function of high biological priority was studied in cultured cerebrovascular endothelium. The use of a multiparameter flow cytometric system allowed simultaneous measurements of cell volume, viability, and membrane potential or intracellular pH. Endothelium, the cellular constituent of the blood-brain barrier (BBB), swells immediately on exposure to low osmolality. This is associated with membrane depolarization and a fall of intracellular pH. Within 30-60 min, cell volume and membrane potential recover completely, although the extracellular osmolality is kept low. Intracellular pH does not normalize fully. Measurements of intracellular K+ and Na+ concentrations reveal their involvement in the regulatory process. The findings strongly suggest that the cerebrovascular endothelium has a highly effective built-in capacity for homeostatic control essential for normal BBB function. |
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