Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials
Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scannin...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1985 |
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| Umfang: |
14 Ill. 7 |
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| Reproduktion: |
Wiley InterScience Backfile Collection 1832-2000 |
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| Übergeordnetes Werk: |
in: Journal of Electron Microscopy Technique - New York, NY : Wiley, 2(1985) vom: Mai, Seite 489-495 |
| Übergeordnetes Werk: |
volume:2 ; year:1985 ; month:05 ; pages:489-495 ; extent:7 |
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NLEJ160859441 |
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| 520 | |a Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. | ||
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(DE-627)NLEJ160859441 DE-627 ger DE-627 rakwb eng Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials 1985 14 Ill. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. Wiley InterScience Backfile Collection 1832-2000 Russell, Scott D. oth Daghlian, Charles P. oth in Journal of Electron Microscopy Technique New York, NY : Wiley 2(1985) vom: Mai, Seite 489-495 (DE-627)NLEJ159071011 0741-0581 nnns volume:2 year:1985 month:05 pages:489-495 extent:7 http://dx.doi.org/10.1002/jemt.1060020511 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 2 1985 5 489-495 7 |
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(DE-627)NLEJ160859441 DE-627 ger DE-627 rakwb eng Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials 1985 14 Ill. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. Wiley InterScience Backfile Collection 1832-2000 Russell, Scott D. oth Daghlian, Charles P. oth in Journal of Electron Microscopy Technique New York, NY : Wiley 2(1985) vom: Mai, Seite 489-495 (DE-627)NLEJ159071011 0741-0581 nnns volume:2 year:1985 month:05 pages:489-495 extent:7 http://dx.doi.org/10.1002/jemt.1060020511 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 2 1985 5 489-495 7 |
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(DE-627)NLEJ160859441 DE-627 ger DE-627 rakwb eng Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials 1985 14 Ill. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. Wiley InterScience Backfile Collection 1832-2000 Russell, Scott D. oth Daghlian, Charles P. oth in Journal of Electron Microscopy Technique New York, NY : Wiley 2(1985) vom: Mai, Seite 489-495 (DE-627)NLEJ159071011 0741-0581 nnns volume:2 year:1985 month:05 pages:489-495 extent:7 http://dx.doi.org/10.1002/jemt.1060020511 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 2 1985 5 489-495 7 |
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(DE-627)NLEJ160859441 DE-627 ger DE-627 rakwb eng Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials 1985 14 Ill. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. Wiley InterScience Backfile Collection 1832-2000 Russell, Scott D. oth Daghlian, Charles P. oth in Journal of Electron Microscopy Technique New York, NY : Wiley 2(1985) vom: Mai, Seite 489-495 (DE-627)NLEJ159071011 0741-0581 nnns volume:2 year:1985 month:05 pages:489-495 extent:7 http://dx.doi.org/10.1002/jemt.1060020511 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 2 1985 5 489-495 7 |
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(DE-627)NLEJ160859441 DE-627 ger DE-627 rakwb eng Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials 1985 14 Ill. 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. Wiley InterScience Backfile Collection 1832-2000 Russell, Scott D. oth Daghlian, Charles P. oth in Journal of Electron Microscopy Technique New York, NY : Wiley 2(1985) vom: Mai, Seite 489-495 (DE-627)NLEJ159071011 0741-0581 nnns volume:2 year:1985 month:05 pages:489-495 extent:7 http://dx.doi.org/10.1002/jemt.1060020511 text/html Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-WIS GBV_NL_ARTICLE AR 2 1985 5 489-495 7 |
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scanning electron microscopic observations on deembedded biological tissue sections: comparison of different fixatives and embedding materials |
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Scanning electron microscopic observations on deembedded biological tissue sections: Comparison of different fixatives and embedding materials |
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Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. |
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Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. |
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Conventional plant histological specimens fixed in formalin-acetic acid-alcohol, chromic acid-acetic acid-formaldehyde, or glutaraldehyde-osmium and embedded in either paraffin or plastic are examined as possible rapid methods for providing an alternative image of cellular structure by using scanning electron microscopy. Using the mitotic figures of actively growing onion root tips as a study specimen, the organization of the nucleus and spindle apparatus is reasonably well preserved as compared with isolated mitotic spindles and studies of mitosis in endosperm tissue. Relief of internal structure in this technique is obtained through the coagulant nature of the fixative. Used judiciously, this technique can reveal aspects of the three-dimensional nature of internal tissue structure that may otherwise be difficult to discern. |
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