Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry
Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1984 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Analytica Chimica Acta - Amsterdam : Elsevier, 162(1984), Seite 275-283 |
| Übergeordnetes Werk: |
volume:162 ; year:1984 ; pages:275-283 |
| Links: |
|---|
| Katalog-ID: |
NLEJ173484581 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ173484581 | ||
| 003 | DE-627 | ||
| 005 | 20210705190306.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070505s1984 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ173484581 | ||
| 035 | |a (DE-599)GBVNLZ173484581 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| 264 | 1 | |c 1984 | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. | ||
| 533 | |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 | ||
| 700 | 1 | |a Bright, F.V. |4 oth | |
| 700 | 1 | |a McGown, L.B. |4 oth | |
| 773 | 0 | 8 | |i in |t Analytica Chimica Acta |d Amsterdam : Elsevier |g 162(1984), Seite 275-283 |w (DE-627)NLEJ173450067 |w (DE-600)1483436-4 |x 0003-2670 |7 nnns |
| 773 | 1 | 8 | |g volume:162 |g year:1984 |g pages:275-283 |
| 856 | 4 | 0 | |u http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 |
| 912 | |a GBV_USEFLAG_H | ||
| 912 | |a ZDB-1-SDJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 162 |j 1984 |h 275-283 | ||
| matchkey_str |
article:00032670:1984----::lmntooblrbnnefrnenloierceemntoofursenyhsrs |
|---|---|
| hierarchy_sort_str |
1984 |
| publishDate |
1984 |
| allfields |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 DE-627 ger DE-627 rakwb eng Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Analytica Chimica Acta Amsterdam : Elsevier 162(1984), Seite 275-283 (DE-627)NLEJ173450067 (DE-600)1483436-4 0003-2670 nnns volume:162 year:1984 pages:275-283 http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 162 1984 275-283 |
| spelling |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 DE-627 ger DE-627 rakwb eng Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Analytica Chimica Acta Amsterdam : Elsevier 162(1984), Seite 275-283 (DE-627)NLEJ173450067 (DE-600)1483436-4 0003-2670 nnns volume:162 year:1984 pages:275-283 http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 162 1984 275-283 |
| allfields_unstemmed |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 DE-627 ger DE-627 rakwb eng Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Analytica Chimica Acta Amsterdam : Elsevier 162(1984), Seite 275-283 (DE-627)NLEJ173450067 (DE-600)1483436-4 0003-2670 nnns volume:162 year:1984 pages:275-283 http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 162 1984 275-283 |
| allfieldsGer |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 DE-627 ger DE-627 rakwb eng Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Analytica Chimica Acta Amsterdam : Elsevier 162(1984), Seite 275-283 (DE-627)NLEJ173450067 (DE-600)1483436-4 0003-2670 nnns volume:162 year:1984 pages:275-283 http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 162 1984 275-283 |
| allfieldsSound |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 DE-627 ger DE-627 rakwb eng Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Analytica Chimica Acta Amsterdam : Elsevier 162(1984), Seite 275-283 (DE-627)NLEJ173450067 (DE-600)1483436-4 0003-2670 nnns volume:162 year:1984 pages:275-283 http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 162 1984 275-283 |
| language |
English |
| source |
in Analytica Chimica Acta 162(1984), Seite 275-283 volume:162 year:1984 pages:275-283 |
| sourceStr |
in Analytica Chimica Acta 162(1984), Seite 275-283 volume:162 year:1984 pages:275-283 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Analytica Chimica Acta |
| authorswithroles_txt_mv |
Bright, F.V. @@oth@@ McGown, L.B. @@oth@@ |
| publishDateDaySort_date |
1984-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ173450067 |
| id |
NLEJ173484581 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ173484581</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210705190306.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070505s1984 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ173484581</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ173484581</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1984</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bright, F.V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">McGown, L.B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Analytica Chimica Acta</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">162(1984), Seite 275-283</subfield><subfield code="w">(DE-627)NLEJ173450067</subfield><subfield code="w">(DE-600)1483436-4</subfield><subfield code="x">0003-2670</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:162</subfield><subfield code="g">year:1984</subfield><subfield code="g">pages:275-283</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">162</subfield><subfield code="j">1984</subfield><subfield code="h">275-283</subfield></datafield></record></collection>
|
| series2 |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ173450067 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
0003-2670 |
| topic_title |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
f b fb l m lm |
| hierarchy_parent_title |
Analytica Chimica Acta |
| hierarchy_parent_id |
NLEJ173450067 |
| hierarchy_top_title |
Analytica Chimica Acta |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ173450067 (DE-600)1483436-4 |
| title |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| spellingShingle |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| ctrlnum |
(DE-627)NLEJ173484581 (DE-599)GBVNLZ173484581 |
| title_full |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| journal |
Analytica Chimica Acta |
| journalStr |
Analytica Chimica Acta |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1984 |
| contenttype_str_mv |
zzz |
| container_start_page |
275 |
| container_volume |
162 |
| format_se |
Elektronische Aufsätze |
| title_sort |
elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| title_auth |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| abstract |
Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. |
| abstractGer |
Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. |
| abstract_unstemmed |
Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM. |
| collection_details |
GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
| title_short |
Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry |
| url |
http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8 |
| remote_bool |
true |
| author2 |
Bright, F.V. McGown, L.B. |
| author2Str |
Bright, F.V. McGown, L.B. |
| ppnlink |
NLEJ173450067 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth |
| up_date |
2024-07-05T22:04:38.094Z |
| _version_ |
1803778342879494144 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ173484581</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210705190306.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070505s1984 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ173484581</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ173484581</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Elimination of bilirubin interference in fluorimetric determination of fluorescein by phase-resolved fluorescence spectrometry</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1984</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Phase-resolved fluorescence spectrometry is used to eliminate bilirubin interference in the fluorimetric quantitation of fluorescein, despite extensive excitation and emission spectral overlap of the two species. In this method, it is the difference in fluorescence lifetimes of the two species that provides the selectivity parameter. Results are given for a nulling approach and for a multiple phase-angle approach. In the nulling approach, intensity is measured at a detector phase angle at which the intensity for bilirubin is zero. In the multiple phase-angle approach, intensities at several phase angles are used to generate a matrix which is solved for the concentrations of fluorescein and bilirubin. Although a steady-state method yielded low results for fluorescein concentration in the presence of bilirubin concentration about 3 μM, the phase-resolved methods yielded correct values for fluorescein concentration with bilirubin concentration up to 10 μM.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bright, F.V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">McGown, L.B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Analytica Chimica Acta</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">162(1984), Seite 275-283</subfield><subfield code="w">(DE-627)NLEJ173450067</subfield><subfield code="w">(DE-600)1483436-4</subfield><subfield code="x">0003-2670</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:162</subfield><subfield code="g">year:1984</subfield><subfield code="g">pages:275-283</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/S0003-2670(00)84248-8</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">162</subfield><subfield code="j">1984</subfield><subfield code="h">275-283</subfield></datafield></record></collection>
|
| score |
7.401355 |