Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography
A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and ph...
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1993 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Journal of Chromatography A - Amsterdam : Elsevier, 645(1993), 1, Seite 189-192 |
| Übergeordnetes Werk: |
volume:645 ; year:1993 ; number:1 ; pages:189-192 |
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| 520 | |a A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. | ||
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(DE-627)NLEJ174306814 (DE-599)GBVNLZ174306814 DE-627 ger DE-627 rakwb eng Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Leonard, S.A. oth Wong, S.C. oth Nyce, J.W. oth in Journal of Chromatography A Amsterdam : Elsevier 645(1993), 1, Seite 189-192 (DE-627)NLEJ17403282X (DE-600)1491247-8 0021-9673 nnns volume:645 year:1993 number:1 pages:189-192 http://linkinghub.elsevier.com/retrieve/pii/0021-9673(93)80635-L GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 645 1993 1 189-192 |
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(DE-627)NLEJ174306814 (DE-599)GBVNLZ174306814 DE-627 ger DE-627 rakwb eng Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Leonard, S.A. oth Wong, S.C. oth Nyce, J.W. oth in Journal of Chromatography A Amsterdam : Elsevier 645(1993), 1, Seite 189-192 (DE-627)NLEJ17403282X (DE-600)1491247-8 0021-9673 nnns volume:645 year:1993 number:1 pages:189-192 http://linkinghub.elsevier.com/retrieve/pii/0021-9673(93)80635-L GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 645 1993 1 189-192 |
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(DE-627)NLEJ174306814 (DE-599)GBVNLZ174306814 DE-627 ger DE-627 rakwb eng Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Leonard, S.A. oth Wong, S.C. oth Nyce, J.W. oth in Journal of Chromatography A Amsterdam : Elsevier 645(1993), 1, Seite 189-192 (DE-627)NLEJ17403282X (DE-600)1491247-8 0021-9673 nnns volume:645 year:1993 number:1 pages:189-192 http://linkinghub.elsevier.com/retrieve/pii/0021-9673(93)80635-L GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 645 1993 1 189-192 |
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(DE-627)NLEJ174306814 (DE-599)GBVNLZ174306814 DE-627 ger DE-627 rakwb eng Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Leonard, S.A. oth Wong, S.C. oth Nyce, J.W. oth in Journal of Chromatography A Amsterdam : Elsevier 645(1993), 1, Seite 189-192 (DE-627)NLEJ17403282X (DE-600)1491247-8 0021-9673 nnns volume:645 year:1993 number:1 pages:189-192 http://linkinghub.elsevier.com/retrieve/pii/0021-9673(93)80635-L GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 645 1993 1 189-192 |
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(DE-627)NLEJ174306814 (DE-599)GBVNLZ174306814 DE-627 ger DE-627 rakwb eng Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Leonard, S.A. oth Wong, S.C. oth Nyce, J.W. oth in Journal of Chromatography A Amsterdam : Elsevier 645(1993), 1, Seite 189-192 (DE-627)NLEJ17403282X (DE-600)1491247-8 0021-9673 nnns volume:645 year:1993 number:1 pages:189-192 http://linkinghub.elsevier.com/retrieve/pii/0021-9673(93)80635-L GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 645 1993 1 189-192 |
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Quantitation of 5-methylcytosine by one-dimensional high-performance thin-layer chromatography |
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A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. |
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A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. |
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A method for the quantitative analysis of DNA 5-methylcytosine by one-dimensional high-performance thin-layer chromatography using alkylamino modified silica (HPTLC-NH"2) plates is described. The preparative method is simple, involving enzymatic digestion of DNA with micrococcal nuclease and phosphodiesterase II to 3'-monophosphate nucleosides, conversion by T"4 polynucleotide kiiiase to ^3^2P-labeled 3',5'-bisphosphate nucleosides, and chromatographic separation of nuclease P"1-cleaved 5'-monophosphate nucleosides. The weak, basic anion exchanger property of the HPTLC-NH"2 plate enables separation of multiple samples in one dimension, whereas traditional polyethyleneimine cellulose plates require development of individual samples in two dimensions for analysis of 5-methylcytosine. |
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