Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy
A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the diffe...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1985 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Talanta - Amsterdam : Elsevier, 32(1985), 1, Seite 15-18 |
| Übergeordnetes Werk: |
volume:32 ; year:1985 ; number:1 ; pages:15-18 |
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| 520 | |a A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. | ||
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(DE-627)NLEJ174544588 (DE-599)GBVNLZ174544588 DE-627 ger DE-627 rakwb eng Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy 1985 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Talanta Amsterdam : Elsevier 32(1985), 1, Seite 15-18 (DE-627)NLEJ174527721 (DE-600)1500969-5 0039-9140 nnns volume:32 year:1985 number:1 pages:15-18 http://linkinghub.elsevier.com/retrieve/pii/0039-9140(85)80006-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 32 1985 1 15-18 |
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(DE-627)NLEJ174544588 (DE-599)GBVNLZ174544588 DE-627 ger DE-627 rakwb eng Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy 1985 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Talanta Amsterdam : Elsevier 32(1985), 1, Seite 15-18 (DE-627)NLEJ174527721 (DE-600)1500969-5 0039-9140 nnns volume:32 year:1985 number:1 pages:15-18 http://linkinghub.elsevier.com/retrieve/pii/0039-9140(85)80006-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 32 1985 1 15-18 |
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(DE-627)NLEJ174544588 (DE-599)GBVNLZ174544588 DE-627 ger DE-627 rakwb eng Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy 1985 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Talanta Amsterdam : Elsevier 32(1985), 1, Seite 15-18 (DE-627)NLEJ174527721 (DE-600)1500969-5 0039-9140 nnns volume:32 year:1985 number:1 pages:15-18 http://linkinghub.elsevier.com/retrieve/pii/0039-9140(85)80006-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 32 1985 1 15-18 |
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(DE-627)NLEJ174544588 (DE-599)GBVNLZ174544588 DE-627 ger DE-627 rakwb eng Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy 1985 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Talanta Amsterdam : Elsevier 32(1985), 1, Seite 15-18 (DE-627)NLEJ174527721 (DE-600)1500969-5 0039-9140 nnns volume:32 year:1985 number:1 pages:15-18 http://linkinghub.elsevier.com/retrieve/pii/0039-9140(85)80006-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 32 1985 1 15-18 |
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(DE-627)NLEJ174544588 (DE-599)GBVNLZ174544588 DE-627 ger DE-627 rakwb eng Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy 1985 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bright, F.V. oth McGown, L.B. oth in Talanta Amsterdam : Elsevier 32(1985), 1, Seite 15-18 (DE-627)NLEJ174527721 (DE-600)1500969-5 0039-9140 nnns volume:32 year:1985 number:1 pages:15-18 http://linkinghub.elsevier.com/retrieve/pii/0039-9140(85)80006-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 32 1985 1 15-18 |
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homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy |
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Homogeneous immunoassay of phenobarbital by phase-resolved fluorescence spectroscopy |
| abstract |
A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. |
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A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. |
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A new technique for determination of phenobarbital by homogeneous fluoroimmunoassay is described. Simultaneous determination of free and antibody-bound fluorescein-labelled phenobarbital is accomplished by making phase-resolved fluorescence measurements, in which selectivity is provided by the difference between the fluorescence lifetimes of the two species, as well as by the difference in fluorescence intensities. The technique requires no specialized reagents other than those inherent in immunoassay (antibody, antigen and labelled antigen). Problems due to non-specific binding of labelled antigen to sample matrix components can be alleviated by addition of a relative excess of albumin. |
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