Purification and methylation analysis of cell wall material from Solanum tuberosum
Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydro...
Ausführliche Beschreibung
Gespeichert in:
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Englisch |
| Erschienen: |
1978 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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in: Phytochemistry - Amsterdam : Elsevier, 17(1978), 4, Seite 745-752 |
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volume:17 ; year:1978 ; number:4 ; pages:745-752 |
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| 520 | |a Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. | ||
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(DE-627)NLEJ175659230 (DE-599)GBVNLZ175659230 DE-627 ger DE-627 rakwb eng Purification and methylation analysis of cell wall material from Solanum tuberosum 1978 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Ring, S.G. oth Selvendran, R.R. oth in Phytochemistry Amsterdam : Elsevier 17(1978), 4, Seite 745-752 (DE-627)NLEJ17552694X (DE-600)2000313-4 0031-9422 nnns volume:17 year:1978 number:4 pages:745-752 http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 17 1978 4 745-752 |
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(DE-627)NLEJ175659230 (DE-599)GBVNLZ175659230 DE-627 ger DE-627 rakwb eng Purification and methylation analysis of cell wall material from Solanum tuberosum 1978 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Ring, S.G. oth Selvendran, R.R. oth in Phytochemistry Amsterdam : Elsevier 17(1978), 4, Seite 745-752 (DE-627)NLEJ17552694X (DE-600)2000313-4 0031-9422 nnns volume:17 year:1978 number:4 pages:745-752 http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 17 1978 4 745-752 |
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(DE-627)NLEJ175659230 (DE-599)GBVNLZ175659230 DE-627 ger DE-627 rakwb eng Purification and methylation analysis of cell wall material from Solanum tuberosum 1978 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Ring, S.G. oth Selvendran, R.R. oth in Phytochemistry Amsterdam : Elsevier 17(1978), 4, Seite 745-752 (DE-627)NLEJ17552694X (DE-600)2000313-4 0031-9422 nnns volume:17 year:1978 number:4 pages:745-752 http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 17 1978 4 745-752 |
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(DE-627)NLEJ175659230 (DE-599)GBVNLZ175659230 DE-627 ger DE-627 rakwb eng Purification and methylation analysis of cell wall material from Solanum tuberosum 1978 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Ring, S.G. oth Selvendran, R.R. oth in Phytochemistry Amsterdam : Elsevier 17(1978), 4, Seite 745-752 (DE-627)NLEJ17552694X (DE-600)2000313-4 0031-9422 nnns volume:17 year:1978 number:4 pages:745-752 http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 17 1978 4 745-752 |
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(DE-627)NLEJ175659230 (DE-599)GBVNLZ175659230 DE-627 ger DE-627 rakwb eng Purification and methylation analysis of cell wall material from Solanum tuberosum 1978 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Ring, S.G. oth Selvendran, R.R. oth in Phytochemistry Amsterdam : Elsevier 17(1978), 4, Seite 745-752 (DE-627)NLEJ17552694X (DE-600)2000313-4 0031-9422 nnns volume:17 year:1978 number:4 pages:745-752 http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 17 1978 4 745-752 |
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Purification and methylation analysis of cell wall material from Solanum tuberosum |
| abstract |
Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. |
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Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. |
| abstract_unstemmed |
Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ175659230</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706003726.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070505s1978 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ175659230</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ175659230</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Purification and methylation analysis of cell wall material from Solanum tuberosum</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1978</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Cell wall material (CWM) of potatoes was prepared by sequentially extracting the wet ball-milled tissue with 1 % aq. Na deoxycholate, PhOHHOAcH"2O and 90 % (v/v) aq. DMSO. The purity of the CWM (e.g. absence of residual starch) was established by carbohydrate analysis using different acid hydrolysis conditions and by methylation studies. The partially methylated alditol acetates from the CHCl"3MeOH soluble fraction (S) of the methylated CWM were separated into 15 main peaks by GLC. Fourteen of these peaks were carbohydrate derivatives and the identity of most of these was established by MS. Reduction of the hydrolysate of S with NaBD"4 was used to identify the carbohydrate derivatives present in peaks 7 and 11 above. The occurrence of 4-linked galacturonosyl residues in the methylated polymers was established after reduction of S with LiAlH"4 and LiAlD"4. The main glycosidic linkages present in the non-cellulosic polysaccharides of the wall in descending order of concentration are: 4-linked galactose, 4-linked galacturonic acid, 5-linked arabinose and 4,6-linked glucose. The major branch points are those through 0-6 of glucose and 0-4 of rhamnose. Arabinose, galactose and xylose residues constituted the non-reducing ends. Graded acid hydrolysis of the CWM made it possible to assess the relative strengths of some of the glycosidic linkages. The general structural features of the CWM are discussed in the light of these results.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ring, S.G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Selvendran, R.R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Phytochemistry</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">17(1978), 4, Seite 745-752</subfield><subfield code="w">(DE-627)NLEJ17552694X</subfield><subfield code="w">(DE-600)2000313-4</subfield><subfield code="x">0031-9422</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:17</subfield><subfield code="g">year:1978</subfield><subfield code="g">number:4</subfield><subfield code="g">pages:745-752</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)94219-5</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">17</subfield><subfield code="j">1978</subfield><subfield code="e">4</subfield><subfield code="h">745-752</subfield></datafield></record></collection>
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7.398863 |