On the sealing of gas-filled glass ampoules
Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,&...
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1975 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Cryobiology - Amsterdam : Elsevier, 12(1975), 1, Seite 1-14 |
| Übergeordnetes Werk: |
volume:12 ; year:1975 ; number:1 ; pages:1-14 |
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| 520 | |a Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. | ||
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(DE-627)NLEJ183064755 (DE-599)GBVNLZ183064755 DE-627 ger DE-627 rakwb eng On the sealing of gas-filled glass ampoules 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Greiff, D. oth Melton, H. oth Rowe, T.W.G. oth in Cryobiology Amsterdam : Elsevier 12(1975), 1, Seite 1-14 (DE-627)NLEJ176926798 (DE-600)1463192-1 0011-2240 nnns volume:12 year:1975 number:1 pages:1-14 http://dx.doi.org/10.1016/0011-2240(75)90036-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 12 1975 1 1-14 |
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(DE-627)NLEJ183064755 (DE-599)GBVNLZ183064755 DE-627 ger DE-627 rakwb eng On the sealing of gas-filled glass ampoules 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Greiff, D. oth Melton, H. oth Rowe, T.W.G. oth in Cryobiology Amsterdam : Elsevier 12(1975), 1, Seite 1-14 (DE-627)NLEJ176926798 (DE-600)1463192-1 0011-2240 nnns volume:12 year:1975 number:1 pages:1-14 http://dx.doi.org/10.1016/0011-2240(75)90036-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 12 1975 1 1-14 |
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(DE-627)NLEJ183064755 (DE-599)GBVNLZ183064755 DE-627 ger DE-627 rakwb eng On the sealing of gas-filled glass ampoules 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Greiff, D. oth Melton, H. oth Rowe, T.W.G. oth in Cryobiology Amsterdam : Elsevier 12(1975), 1, Seite 1-14 (DE-627)NLEJ176926798 (DE-600)1463192-1 0011-2240 nnns volume:12 year:1975 number:1 pages:1-14 http://dx.doi.org/10.1016/0011-2240(75)90036-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 12 1975 1 1-14 |
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(DE-627)NLEJ183064755 (DE-599)GBVNLZ183064755 DE-627 ger DE-627 rakwb eng On the sealing of gas-filled glass ampoules 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Greiff, D. oth Melton, H. oth Rowe, T.W.G. oth in Cryobiology Amsterdam : Elsevier 12(1975), 1, Seite 1-14 (DE-627)NLEJ176926798 (DE-600)1463192-1 0011-2240 nnns volume:12 year:1975 number:1 pages:1-14 http://dx.doi.org/10.1016/0011-2240(75)90036-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 12 1975 1 1-14 |
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(DE-627)NLEJ183064755 (DE-599)GBVNLZ183064755 DE-627 ger DE-627 rakwb eng On the sealing of gas-filled glass ampoules 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Greiff, D. oth Melton, H. oth Rowe, T.W.G. oth in Cryobiology Amsterdam : Elsevier 12(1975), 1, Seite 1-14 (DE-627)NLEJ176926798 (DE-600)1463192-1 0011-2240 nnns volume:12 year:1975 number:1 pages:1-14 http://dx.doi.org/10.1016/0011-2240(75)90036-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 12 1975 1 1-14 |
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On the sealing of gas-filled glass ampoules |
| abstract |
Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. |
| abstractGer |
Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. |
| abstract_unstemmed |
Studies in our laboratories involved the placing of argon-filled hand-sealed glass ampoules (tip-, balloon-, or draw-sealed) containing biological materials dried by sublimation of ice in vacuo in water baths at elevated temperatures. Although these ampoules tested negative for ''leakers,'' many ampoules upon removal from the water bath contained beads of moisture. To determine if the water within the ampoules entered through openings in the closed ends, we used a laser imaging apparatus to examine the sealed ends. We carried out studies also on machine-sealed ampoules.Two modes of laser imaging were used: dark-field imaging and interference imaging. In tip-sealed ampoules, uniform, long channels were found in the gatherings of glass at the ends of the ampoules; the lengths of the channels were approximately ten times their diameters. In balloon-sealed ampoules, pore-like openings were found; the lengths of the pores were approximately four times their diameters. In draw-sealed ampoules, channels of small diameters were observed; the lengths of the open pathways were approximately 30 times their diameters. Based on the sizes of the images obtained with laser imaging, the magnifications used for photographic reproductions and the original measurements of the sealed ampoules we estimated the openings in tip-sealed and balloon-sealed ampoules to range from 5 to 8 μm and the channels in draw-sealed ampoules to be less than 3 μm in diameter, The diameters of the helix-like openings in machine-sealed ampoules were less than 5 μm.To prevent the migration of molecules into and out of ampoules, we sought for a barrier that could be interposed between the external environments of the ampoules. Many liquid formulations of natural and man-made elastomers were tested; neoprene dissolved in toluol was found best. |
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On the sealing of gas-filled glass ampoules |
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Greiff, D. Melton, H. Rowe, T.W.G. |
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