Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro
Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, act...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1994 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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in: Experimental Cell Research - Amsterdam : Elsevier, 210(1994), 2, Seite 224-229 |
| Übergeordnetes Werk: |
volume:210 ; year:1994 ; number:2 ; pages:224-229 |
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| 520 | |a Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. | ||
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(DE-627)NLEJ18365191X (DE-599)GBVNLZ18365191X DE-627 ger DE-627 rakwb eng Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Luyten, F.P. oth Chen, P. oth Paralkar, V. oth Reddi, A.H. oth in Experimental Cell Research Amsterdam : Elsevier 210(1994), 2, Seite 224-229 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:210 year:1994 number:2 pages:224-229 http://dx.doi.org/10.1006/excr.1994.1033 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 210 1994 2 224-229 |
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(DE-627)NLEJ18365191X (DE-599)GBVNLZ18365191X DE-627 ger DE-627 rakwb eng Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Luyten, F.P. oth Chen, P. oth Paralkar, V. oth Reddi, A.H. oth in Experimental Cell Research Amsterdam : Elsevier 210(1994), 2, Seite 224-229 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:210 year:1994 number:2 pages:224-229 http://dx.doi.org/10.1006/excr.1994.1033 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 210 1994 2 224-229 |
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(DE-627)NLEJ18365191X (DE-599)GBVNLZ18365191X DE-627 ger DE-627 rakwb eng Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Luyten, F.P. oth Chen, P. oth Paralkar, V. oth Reddi, A.H. oth in Experimental Cell Research Amsterdam : Elsevier 210(1994), 2, Seite 224-229 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:210 year:1994 number:2 pages:224-229 http://dx.doi.org/10.1006/excr.1994.1033 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 210 1994 2 224-229 |
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(DE-627)NLEJ18365191X (DE-599)GBVNLZ18365191X DE-627 ger DE-627 rakwb eng Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Luyten, F.P. oth Chen, P. oth Paralkar, V. oth Reddi, A.H. oth in Experimental Cell Research Amsterdam : Elsevier 210(1994), 2, Seite 224-229 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:210 year:1994 number:2 pages:224-229 http://dx.doi.org/10.1006/excr.1994.1033 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 210 1994 2 224-229 |
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(DE-627)NLEJ18365191X (DE-599)GBVNLZ18365191X DE-627 ger DE-627 rakwb eng Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Luyten, F.P. oth Chen, P. oth Paralkar, V. oth Reddi, A.H. oth in Experimental Cell Research Amsterdam : Elsevier 210(1994), 2, Seite 224-229 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:210 year:1994 number:2 pages:224-229 http://dx.doi.org/10.1006/excr.1994.1033 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 210 1994 2 224-229 |
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recombinant bone morphogenetic protein-4, transforming growth factor-β"1, and activin a enhance the cartilage phenotype of articular chondrocytes in vitro |
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Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro |
| abstract |
Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. |
| abstractGer |
Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. |
| abstract_unstemmed |
Bone morphogenetic protein-4 (BMP-4) is a member of the transforming growth factor-β (TGF-β) supergene family and is characterized by its ability to induce singly de novo cartilage and bone in vivo. The influence of recombinant bone morphogenetic protein-4 and some related members, TGF-β"1, activin A, and inhibin A, on articular chondrocyte metabolism in the presence and absence of extracellular matrix has been examined. BMP-4 and TGF-β"1 stimulated [^3^5S]-sulfate incorporation in a dose-dependent manner in short-term monolayer, micromass, and explant cultures. Activin A showed a slight but significant stimulation of proteoglycan synthesis while inhibin A decreased metabolic activity. The effects observed were most pronounced in the explant culture system. Although the relative influence of the growth factors was less apparent in chondrocytes isolated from adult cartilage, the qualitative responses were similar with cells obtained from young animals. The maintenance and enhancement of the cartilage phenotype was further investigated by Northern blot analysis. BMP-4 and TGF-β"1 increased the levels of expression of type II collagen and proteoglycan aggrecan in short-term cultures, while activin A and inhibin A did not affect these parameters significantly when compared to serum-free control cultures. Binding experiments with ^1^2^5I-BMP-4, revealed the presence of specific, high-affinity binding sites with an apparent dissociation constant of 110 pM and about 6000 receptors per cell. Chemical cross-linking showed the presence of three components (apparent size 200, 90, and 70 kDa), demonstrating the presence of functional receptors for BMP-4 on primary articular chondrocytes. |
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Recombinant Bone Morphogenetic Protein-4, Transforming Growth Factor-β"1, and Activin A Enhance the Cartilage Phenotype of Articular Chondrocytes in Vitro |
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http://dx.doi.org/10.1006/excr.1994.1033 |
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Luyten, F.P. Chen, P. Paralkar, V. Reddi, A.H. |
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