Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations
Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and sec...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1993 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Experimental Cell Research - Amsterdam : Elsevier, 205(1993), 1, Seite 16-24 |
| Übergeordnetes Werk: |
volume:205 ; year:1993 ; number:1 ; pages:16-24 |
| Links: |
|---|
| Katalog-ID: |
NLEJ183655915 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ183655915 | ||
| 003 | DE-627 | ||
| 005 | 20210706210552.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070506s1993 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ183655915 | ||
| 035 | |a (DE-599)GBVNLZ183655915 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| 264 | 1 | |c 1993 | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. | ||
| 533 | |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 | ||
| 700 | 1 | |a Murphy, P.G. |4 oth | |
| 700 | 1 | |a Frank, C.B. |4 oth | |
| 700 | 1 | |a Hart, D.A. |4 oth | |
| 773 | 0 | 8 | |i in |t Experimental Cell Research |d Amsterdam : Elsevier |g 205(1993), 1, Seite 16-24 |w (DE-627)NLEJ177031808 |w (DE-600)1466780-0 |x 0014-4827 |7 nnns |
| 773 | 1 | 8 | |g volume:205 |g year:1993 |g number:1 |g pages:16-24 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1006/excr.1993.1052 |
| 912 | |a GBV_USEFLAG_H | ||
| 912 | |a ZDB-1-SDJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 205 |j 1993 |e 1 |h 16-24 | ||
| matchkey_str |
article:00144827:1993----::hrceiainfhpamngnciaosnpamngnciaoihbtrepesdyelioaeforbilgmnady |
|---|---|
| hierarchy_sort_str |
1993 |
| publishDate |
1993 |
| allfields |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 DE-627 ger DE-627 rakwb eng Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Murphy, P.G. oth Frank, C.B. oth Hart, D.A. oth in Experimental Cell Research Amsterdam : Elsevier 205(1993), 1, Seite 16-24 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:205 year:1993 number:1 pages:16-24 http://dx.doi.org/10.1006/excr.1993.1052 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 205 1993 1 16-24 |
| spelling |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 DE-627 ger DE-627 rakwb eng Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Murphy, P.G. oth Frank, C.B. oth Hart, D.A. oth in Experimental Cell Research Amsterdam : Elsevier 205(1993), 1, Seite 16-24 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:205 year:1993 number:1 pages:16-24 http://dx.doi.org/10.1006/excr.1993.1052 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 205 1993 1 16-24 |
| allfields_unstemmed |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 DE-627 ger DE-627 rakwb eng Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Murphy, P.G. oth Frank, C.B. oth Hart, D.A. oth in Experimental Cell Research Amsterdam : Elsevier 205(1993), 1, Seite 16-24 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:205 year:1993 number:1 pages:16-24 http://dx.doi.org/10.1006/excr.1993.1052 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 205 1993 1 16-24 |
| allfieldsGer |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 DE-627 ger DE-627 rakwb eng Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Murphy, P.G. oth Frank, C.B. oth Hart, D.A. oth in Experimental Cell Research Amsterdam : Elsevier 205(1993), 1, Seite 16-24 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:205 year:1993 number:1 pages:16-24 http://dx.doi.org/10.1006/excr.1993.1052 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 205 1993 1 16-24 |
| allfieldsSound |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 DE-627 ger DE-627 rakwb eng Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations 1993 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Murphy, P.G. oth Frank, C.B. oth Hart, D.A. oth in Experimental Cell Research Amsterdam : Elsevier 205(1993), 1, Seite 16-24 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:205 year:1993 number:1 pages:16-24 http://dx.doi.org/10.1006/excr.1993.1052 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 205 1993 1 16-24 |
| language |
English |
| source |
in Experimental Cell Research 205(1993), 1, Seite 16-24 volume:205 year:1993 number:1 pages:16-24 |
| sourceStr |
in Experimental Cell Research 205(1993), 1, Seite 16-24 volume:205 year:1993 number:1 pages:16-24 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Experimental Cell Research |
| authorswithroles_txt_mv |
Murphy, P.G. @@oth@@ Frank, C.B. @@oth@@ Hart, D.A. @@oth@@ |
| publishDateDaySort_date |
1993-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ177031808 |
| id |
NLEJ183655915 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ183655915</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706210552.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1993 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ183655915</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ183655915</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1993</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Murphy, P.G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Frank, C.B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hart, D.A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Experimental Cell Research</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">205(1993), 1, Seite 16-24</subfield><subfield code="w">(DE-627)NLEJ177031808</subfield><subfield code="w">(DE-600)1466780-0</subfield><subfield code="x">0014-4827</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:205</subfield><subfield code="g">year:1993</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:16-24</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1006/excr.1993.1052</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">205</subfield><subfield code="j">1993</subfield><subfield code="e">1</subfield><subfield code="h">16-24</subfield></datafield></record></collection>
|
| series2 |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ177031808 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
0014-4827 |
| topic_title |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
p m pm c f cf d h dh |
| hierarchy_parent_title |
Experimental Cell Research |
| hierarchy_parent_id |
NLEJ177031808 |
| hierarchy_top_title |
Experimental Cell Research |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ177031808 (DE-600)1466780-0 |
| title |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| spellingShingle |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| ctrlnum |
(DE-627)NLEJ183655915 (DE-599)GBVNLZ183655915 |
| title_full |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| journal |
Experimental Cell Research |
| journalStr |
Experimental Cell Research |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1993 |
| contenttype_str_mv |
zzz |
| container_start_page |
16 |
| container_volume |
205 |
| format_se |
Elektronische Aufsätze |
| title_sort |
characterization of the plasminogen activators and plasminogen activator inhibitors expressed by ceils isolated from rabbit ligament and synovial tissues: evidence for unique cell populations |
| title_auth |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| abstract |
Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. |
| abstractGer |
Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. |
| abstract_unstemmed |
Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro. |
| collection_details |
GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
| container_issue |
1 |
| title_short |
Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations |
| url |
http://dx.doi.org/10.1006/excr.1993.1052 |
| remote_bool |
true |
| author2 |
Murphy, P.G. Frank, C.B. Hart, D.A. |
| author2Str |
Murphy, P.G. Frank, C.B. Hart, D.A. |
| ppnlink |
NLEJ177031808 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth |
| up_date |
2024-07-05T22:43:52.915Z |
| _version_ |
1803780812092473344 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ183655915</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706210552.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1993 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ183655915</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ183655915</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of the Plasminogen Activators and Plasminogen Activator Inhibitors Expressed by Ceils Isolated from Rabbit Ligament and Synovial Tissues: Evidence for Unique Cell Populations</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1993</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of a plasminogen activator inhibitor (PAI). The inhibitor was identified as PAI-1 by biochemical and molecular techniques. The expression of both PA and PAI activity in the rabbit ligament fibroblasts increased upon addition of phorbol myristate acetate (PMA). To determine the relationship of the ligament fibroblast population to synovial fibroblasts, comparison of the biochemical characteristics of the cell types was performed. It was determined that synovial fibroblasts differed from ligament fibroblasts with respect to PA/PAI profile, type of collagen secreted, and the amount of hyaluronic acid secreted. More specifically, stimulation of synovial fibroblasts with PMA resulted in the detection of a fibrinolytic activity which was not detected in the conditioned medium from PMA-treated ligament fibroblasts. It was also shown that synovial fibroblasts secreted fourfold more hyaluronic acid than ligament fibroblasts. In addition, it was also determined that synovial fibroblasts secrete more type III collagen than do cells isolated from either the ACL or either MCL. These results support the conclusion that the cells derived from these tissues maintain distinct phenotypes in vitro.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Murphy, P.G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Frank, C.B.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hart, D.A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Experimental Cell Research</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">205(1993), 1, Seite 16-24</subfield><subfield code="w">(DE-627)NLEJ177031808</subfield><subfield code="w">(DE-600)1466780-0</subfield><subfield code="x">0014-4827</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:205</subfield><subfield code="g">year:1993</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:16-24</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1006/excr.1993.1052</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">205</subfield><subfield code="j">1993</subfield><subfield code="e">1</subfield><subfield code="h">16-24</subfield></datafield></record></collection>
|
| score |
7.4018955 |