Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus
The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, th...
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E-Artikel |
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Englisch |
| Erschienen: |
1983 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: General and Comparative Endocrinology - Amsterdam : Elsevier, 49(1983), 2, Seite 277-285 |
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volume:49 ; year:1983 ; number:2 ; pages:277-285 |
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| 245 | 1 | 0 | |a Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus |
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| 520 | |a The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. | ||
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(DE-627)NLEJ183699440 (DE-599)GBVNLZ183699440 DE-627 ger DE-627 rakwb eng Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Van Eys, G.J.J.M. oth Lowik, C.W.G.M. oth Bonga, S.E.W. oth in General and Comparative Endocrinology Amsterdam : Elsevier 49(1983), 2, Seite 277-285 (DE-627)NLEJ177003235 (DE-600)1467679-5 0016-6480 nnns volume:49 year:1983 number:2 pages:277-285 http://dx.doi.org/10.1016/0016-6480(83)90145-4 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 49 1983 2 277-285 |
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(DE-627)NLEJ183699440 (DE-599)GBVNLZ183699440 DE-627 ger DE-627 rakwb eng Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Van Eys, G.J.J.M. oth Lowik, C.W.G.M. oth Bonga, S.E.W. oth in General and Comparative Endocrinology Amsterdam : Elsevier 49(1983), 2, Seite 277-285 (DE-627)NLEJ177003235 (DE-600)1467679-5 0016-6480 nnns volume:49 year:1983 number:2 pages:277-285 http://dx.doi.org/10.1016/0016-6480(83)90145-4 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 49 1983 2 277-285 |
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(DE-627)NLEJ183699440 (DE-599)GBVNLZ183699440 DE-627 ger DE-627 rakwb eng Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Van Eys, G.J.J.M. oth Lowik, C.W.G.M. oth Bonga, S.E.W. oth in General and Comparative Endocrinology Amsterdam : Elsevier 49(1983), 2, Seite 277-285 (DE-627)NLEJ177003235 (DE-600)1467679-5 0016-6480 nnns volume:49 year:1983 number:2 pages:277-285 http://dx.doi.org/10.1016/0016-6480(83)90145-4 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 49 1983 2 277-285 |
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(DE-627)NLEJ183699440 (DE-599)GBVNLZ183699440 DE-627 ger DE-627 rakwb eng Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Van Eys, G.J.J.M. oth Lowik, C.W.G.M. oth Bonga, S.E.W. oth in General and Comparative Endocrinology Amsterdam : Elsevier 49(1983), 2, Seite 277-285 (DE-627)NLEJ177003235 (DE-600)1467679-5 0016-6480 nnns volume:49 year:1983 number:2 pages:277-285 http://dx.doi.org/10.1016/0016-6480(83)90145-4 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 49 1983 2 277-285 |
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(DE-627)NLEJ183699440 (DE-599)GBVNLZ183699440 DE-627 ger DE-627 rakwb eng Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Van Eys, G.J.J.M. oth Lowik, C.W.G.M. oth Bonga, S.E.W. oth in General and Comparative Endocrinology Amsterdam : Elsevier 49(1983), 2, Seite 277-285 (DE-627)NLEJ177003235 (DE-600)1467679-5 0016-6480 nnns volume:49 year:1983 number:2 pages:277-285 http://dx.doi.org/10.1016/0016-6480(83)90145-4 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 49 1983 2 277-285 |
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isolation of the biosynthetic products of the pas positive pars intermedia cells in the cichlid teleost sarotherodon mossambicus |
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Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus |
| abstract |
The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. |
| abstractGer |
The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. |
| abstract_unstemmed |
The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells. |
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Van Eys, G.J.J.M. Lowik, C.W.G.M. Bonga, S.E.W. |
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Van Eys, G.J.J.M. Lowik, C.W.G.M. Bonga, S.E.W. |
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NLEJ177003235 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ183699440</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706211329.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1983 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ183699440</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ183699440</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation of the biosynthetic products of the PAS positive pars intermedia cells in the cichlid teleost Sarotherodon mossambicus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1983</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The pars intermedia of teleosts contains two types of granular cells with the predominant type being similar to the pars intermedia cells in other vertebrate groups and containing peptides derived from the pro-opiomelanocortin precursor molecule. The function and products of the second cell type, the PAS positive cells, are unknown. This study reports on the identification of biosynthetic products of the PAS positive cells of the cichlid teleost Sarotherodon mossambicus. The experimental regimen took advantage of earlier morphometric analyses which showed marked differences in metabolic activity of the PAS positive cells resulting from adaptation to different background colours and illumination. Autoradiography at the light microscopic level showed that both cell types of the pars intermedia incorporate labeled amino acids during in vitro incubation. To identify the products synthesized by the PAS positive cells, labeled products of the pars intermedia tissue were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure liquid chromatography. Comparison of pulse incubations of pars intermedia tissue of fish adapted to different backgrounds and conditions of illumination revealed that an increase in the number and metabolic activity of the PAS positive cells, as deduced from morphometric data, was paralleled by an increase of the amount of label incorporated into 27K and 25K molecules. Pulse-chase experiments with pars intermedia lobes of white and black background adapted fish showed that these two products, unlike the other newly synthesized products, were not involved in any precursor-product relationship. Our data, therefore, suggest that the 27K and 25K peptides were synthesized by the PAS positive cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Van Eys, G.J.J.M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lowik, C.W.G.M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bonga, S.E.W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">General and Comparative Endocrinology</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">49(1983), 2, Seite 277-285</subfield><subfield code="w">(DE-627)NLEJ177003235</subfield><subfield code="w">(DE-600)1467679-5</subfield><subfield code="x">0016-6480</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:49</subfield><subfield code="g">year:1983</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:277-285</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1016/0016-6480(83)90145-4</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">49</subfield><subfield code="j">1983</subfield><subfield code="e">2</subfield><subfield code="h">277-285</subfield></datafield></record></collection>
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7.401638 |