Assay of nucleic acids by means of the photometric hemaggregation test
The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggre...
Ausführliche Beschreibung
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1927 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Archives of Biochemistry and Biophysics - Amsterdam : Elsevier, 121(1927), 2, Seite 346-351 |
| Übergeordnetes Werk: |
volume:121 ; year:1927 ; number:2 ; pages:346-351 |
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| 520 | |a The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. | ||
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(DE-627)NLEJ183909615 (DE-599)GBVNLZ183909615 DE-627 ger DE-627 rakwb eng Assay of nucleic acids by means of the photometric hemaggregation test 1927 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Drescher, J. oth in Archives of Biochemistry and Biophysics Amsterdam : Elsevier 121(1927), 2, Seite 346-351 (DE-627)NLEJ177020539 (DE-600)1461378-5 0003-9861 nnns volume:121 year:1927 number:2 pages:346-351 http://dx.doi.org/10.1016/0003-9861(67)90086-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 121 1927 2 346-351 |
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(DE-627)NLEJ183909615 (DE-599)GBVNLZ183909615 DE-627 ger DE-627 rakwb eng Assay of nucleic acids by means of the photometric hemaggregation test 1927 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Drescher, J. oth in Archives of Biochemistry and Biophysics Amsterdam : Elsevier 121(1927), 2, Seite 346-351 (DE-627)NLEJ177020539 (DE-600)1461378-5 0003-9861 nnns volume:121 year:1927 number:2 pages:346-351 http://dx.doi.org/10.1016/0003-9861(67)90086-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 121 1927 2 346-351 |
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(DE-627)NLEJ183909615 (DE-599)GBVNLZ183909615 DE-627 ger DE-627 rakwb eng Assay of nucleic acids by means of the photometric hemaggregation test 1927 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Drescher, J. oth in Archives of Biochemistry and Biophysics Amsterdam : Elsevier 121(1927), 2, Seite 346-351 (DE-627)NLEJ177020539 (DE-600)1461378-5 0003-9861 nnns volume:121 year:1927 number:2 pages:346-351 http://dx.doi.org/10.1016/0003-9861(67)90086-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 121 1927 2 346-351 |
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(DE-627)NLEJ183909615 (DE-599)GBVNLZ183909615 DE-627 ger DE-627 rakwb eng Assay of nucleic acids by means of the photometric hemaggregation test 1927 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Drescher, J. oth in Archives of Biochemistry and Biophysics Amsterdam : Elsevier 121(1927), 2, Seite 346-351 (DE-627)NLEJ177020539 (DE-600)1461378-5 0003-9861 nnns volume:121 year:1927 number:2 pages:346-351 http://dx.doi.org/10.1016/0003-9861(67)90086-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 121 1927 2 346-351 |
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(DE-627)NLEJ183909615 (DE-599)GBVNLZ183909615 DE-627 ger DE-627 rakwb eng Assay of nucleic acids by means of the photometric hemaggregation test 1927 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Drescher, J. oth in Archives of Biochemistry and Biophysics Amsterdam : Elsevier 121(1927), 2, Seite 346-351 (DE-627)NLEJ177020539 (DE-600)1461378-5 0003-9861 nnns volume:121 year:1927 number:2 pages:346-351 http://dx.doi.org/10.1016/0003-9861(67)90086-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 121 1927 2 346-351 |
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Assay of nucleic acids by means of the photometric hemaggregation test |
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The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. |
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The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. |
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The novel technique for the assay of nucleic acids described in this paper utilizes the finding that trypsin-treated human erythrocytes aggregate spontaneously when suspended in a buffered, albumin-containing glucose medium of low ionic strength at low pH and that this aggregation of cells (hemaggregation) is inhibited by nucleic acids. Since treatment of nucleic acids with an excess of the corresponding nuclease results in complete loss of the hemaggregation-inhibiting activity of the acid, the method enables a direct analysis of mixtures of RNA and DNA. The technical performance of the test is described and evidence for the specificity and sensitivity of the method is presented. |
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