Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography
Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a po...
Ausführliche Beschreibung
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E-Artikel |
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Englisch |
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1982 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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Übergeordnetes Werk: |
in: Analytical Biochemistry - Amsterdam : Elsevier, 120(1982), 2, Seite 289-301 |
Übergeordnetes Werk: |
volume:120 ; year:1982 ; number:2 ; pages:289-301 |
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NLEJ183945840 |
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520 | |a Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. | ||
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(DE-627)NLEJ183945840 (DE-599)GBVNLZ183945840 DE-627 ger DE-627 rakwb eng Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Yuan, P.-M. oth Pande, H. oth Clark, B.R. oth Shively, J.E. oth in Analytical Biochemistry Amsterdam : Elsevier 120(1982), 2, Seite 289-301 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:120 year:1982 number:2 pages:289-301 http://dx.doi.org/10.1016/0003-2697(82)90350-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 120 1982 2 289-301 |
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(DE-627)NLEJ183945840 (DE-599)GBVNLZ183945840 DE-627 ger DE-627 rakwb eng Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Yuan, P.-M. oth Pande, H. oth Clark, B.R. oth Shively, J.E. oth in Analytical Biochemistry Amsterdam : Elsevier 120(1982), 2, Seite 289-301 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:120 year:1982 number:2 pages:289-301 http://dx.doi.org/10.1016/0003-2697(82)90350-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 120 1982 2 289-301 |
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(DE-627)NLEJ183945840 (DE-599)GBVNLZ183945840 DE-627 ger DE-627 rakwb eng Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Yuan, P.-M. oth Pande, H. oth Clark, B.R. oth Shively, J.E. oth in Analytical Biochemistry Amsterdam : Elsevier 120(1982), 2, Seite 289-301 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:120 year:1982 number:2 pages:289-301 http://dx.doi.org/10.1016/0003-2697(82)90350-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 120 1982 2 289-301 |
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(DE-627)NLEJ183945840 (DE-599)GBVNLZ183945840 DE-627 ger DE-627 rakwb eng Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Yuan, P.-M. oth Pande, H. oth Clark, B.R. oth Shively, J.E. oth in Analytical Biochemistry Amsterdam : Elsevier 120(1982), 2, Seite 289-301 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:120 year:1982 number:2 pages:289-301 http://dx.doi.org/10.1016/0003-2697(82)90350-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 120 1982 2 289-301 |
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(DE-627)NLEJ183945840 (DE-599)GBVNLZ183945840 DE-627 ger DE-627 rakwb eng Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Yuan, P.-M. oth Pande, H. oth Clark, B.R. oth Shively, J.E. oth in Analytical Biochemistry Amsterdam : Elsevier 120(1982), 2, Seite 289-301 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:120 year:1982 number:2 pages:289-301 http://dx.doi.org/10.1016/0003-2697(82)90350-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 120 1982 2 289-301 |
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microsequence analysis of peptides and proteins - i. preparation of samples by reverse-phase liquid chromatography |
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Microsequence analysis of peptides and proteins - I. Preparation of samples by reverse-phase liquid chromatography |
abstract |
Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. |
abstractGer |
Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. |
abstract_unstemmed |
Reverse-phase supports for the separation of peptides and proteins are compared in two high-performance liquid chromatographic systems. One uses a trifluoroacetic acid-acetonitrile solvent system with a 206-nm detector, and the other uses pyridine-formate or pyridine-acetate and 1-propanol with a postcolumn fluorescence detector. Each system was examined with RP8, RP18, and alkylphenyl supports. In most applications, the trifluoroacetic acid-acetonitrile solvent system used in conjunction with an alkylphenyl column performed best. The use of this system for the preparation of low-microgram amounts of samples for microsequence analysis is illustrated. |
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