Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258
A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as litt...
Ausführliche Beschreibung
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Analytical Biochemistry - Amsterdam : Elsevier, 176(1989), 1, Seite 128-131 |
| Übergeordnetes Werk: |
volume:176 ; year:1989 ; number:1 ; pages:128-131 |
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| 520 | |a A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. | ||
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(DE-627)NLEJ183994434 (DE-599)GBVNLZ183994434 DE-627 ger DE-627 rakwb eng Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Lipman, J.M. oth in Analytical Biochemistry Amsterdam : Elsevier 176(1989), 1, Seite 128-131 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:176 year:1989 number:1 pages:128-131 http://dx.doi.org/10.1016/0003-2697(89)90282-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 176 1989 1 128-131 |
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(DE-627)NLEJ183994434 (DE-599)GBVNLZ183994434 DE-627 ger DE-627 rakwb eng Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Lipman, J.M. oth in Analytical Biochemistry Amsterdam : Elsevier 176(1989), 1, Seite 128-131 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:176 year:1989 number:1 pages:128-131 http://dx.doi.org/10.1016/0003-2697(89)90282-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 176 1989 1 128-131 |
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(DE-627)NLEJ183994434 (DE-599)GBVNLZ183994434 DE-627 ger DE-627 rakwb eng Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Lipman, J.M. oth in Analytical Biochemistry Amsterdam : Elsevier 176(1989), 1, Seite 128-131 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:176 year:1989 number:1 pages:128-131 http://dx.doi.org/10.1016/0003-2697(89)90282-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 176 1989 1 128-131 |
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(DE-627)NLEJ183994434 (DE-599)GBVNLZ183994434 DE-627 ger DE-627 rakwb eng Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Lipman, J.M. oth in Analytical Biochemistry Amsterdam : Elsevier 176(1989), 1, Seite 128-131 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:176 year:1989 number:1 pages:128-131 http://dx.doi.org/10.1016/0003-2697(89)90282-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 176 1989 1 128-131 |
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(DE-627)NLEJ183994434 (DE-599)GBVNLZ183994434 DE-627 ger DE-627 rakwb eng Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Lipman, J.M. oth in Analytical Biochemistry Amsterdam : Elsevier 176(1989), 1, Seite 128-131 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:176 year:1989 number:1 pages:128-131 http://dx.doi.org/10.1016/0003-2697(89)90282-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 176 1989 1 128-131 |
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fluorophotometric quantitation of dna in articular cartilage utilizing hoechst 33258 |
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Fluorophotometric quantitation of DNA in articular cartilage utilizing Hoechst 33258 |
| abstract |
A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. |
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A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. |
| abstract_unstemmed |
A sensitive fluorophotometric assay was developed for the measurement of DNA in articular cartilage. The tissue was digested with Proteinase K and dodecyl sodium sulfate, followed by analysis with Hoechst 33258 dye. DNA content was determined on both fresh and lyophilized material containing as little as 50 ng DNA. The results are comparable to values for other fluorophotometric and spectrophotometric methods reported in the literature. In addition, this method can be incorporated into existing methodology, allowing quantitation of specific glycosaminoglycans in the same cartilage sample in terms of DNA. |
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