A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars
The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1976 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Analytical Biochemistry - Amsterdam : Elsevier, 70(1976), 2, Seite 424-429 |
| Übergeordnetes Werk: |
volume:70 ; year:1976 ; number:2 ; pages:424-429 |
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(DE-627)NLEJ183998170 (DE-599)GBVNLZ183998170 DE-627 ger DE-627 rakwb eng A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mirelman, D. oth in Analytical Biochemistry Amsterdam : Elsevier 70(1976), 2, Seite 424-429 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:70 year:1976 number:2 pages:424-429 http://dx.doi.org/10.1016/0003-2697(76)90465-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 70 1976 2 424-429 |
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(DE-627)NLEJ183998170 (DE-599)GBVNLZ183998170 DE-627 ger DE-627 rakwb eng A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mirelman, D. oth in Analytical Biochemistry Amsterdam : Elsevier 70(1976), 2, Seite 424-429 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:70 year:1976 number:2 pages:424-429 http://dx.doi.org/10.1016/0003-2697(76)90465-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 70 1976 2 424-429 |
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(DE-627)NLEJ183998170 (DE-599)GBVNLZ183998170 DE-627 ger DE-627 rakwb eng A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mirelman, D. oth in Analytical Biochemistry Amsterdam : Elsevier 70(1976), 2, Seite 424-429 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:70 year:1976 number:2 pages:424-429 http://dx.doi.org/10.1016/0003-2697(76)90465-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 70 1976 2 424-429 |
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(DE-627)NLEJ183998170 (DE-599)GBVNLZ183998170 DE-627 ger DE-627 rakwb eng A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mirelman, D. oth in Analytical Biochemistry Amsterdam : Elsevier 70(1976), 2, Seite 424-429 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:70 year:1976 number:2 pages:424-429 http://dx.doi.org/10.1016/0003-2697(76)90465-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 70 1976 2 424-429 |
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(DE-627)NLEJ183998170 (DE-599)GBVNLZ183998170 DE-627 ger DE-627 rakwb eng A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mirelman, D. oth in Analytical Biochemistry Amsterdam : Elsevier 70(1976), 2, Seite 424-429 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:70 year:1976 number:2 pages:424-429 http://dx.doi.org/10.1016/0003-2697(76)90465-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 70 1976 2 424-429 |
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rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars |
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A rapid and simple procedure for the preparation of the two bacterial cell wall peptidoglycan nucleotide precursors labeled in their amino sugars |
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The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. |
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The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. |
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The two bacterial cell wall peptidoglycan precursors UDP-MurNAc-l-Ala-d-iso Glu-l-Lys-d-Ala-d-Ala and UDP-GlcNAc labeled in their amino sugars with either tritium or carbon-14 accumulated in cells of Micrococcus luteus that were incubated for short periods of time in a minimal medium to which [^1^4C]glucose or [^3H]glucose together with Vancomycin were added. The radioactive nucleotides were extracted from the cells with cold trichloroacetic acid, and their purification was achieved by paper electrophoresis followed by paper chromatography. |
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