Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol
A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The us...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1973 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Analytical Biochemistry - Amsterdam : Elsevier, 53(1973), 2, Seite 431-440 |
| Übergeordnetes Werk: |
volume:53 ; year:1973 ; number:2 ; pages:431-440 |
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| 520 | |a A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. | ||
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(DE-627)NLEJ184053269 (DE-599)GBVNLZ184053269 DE-627 ger DE-627 rakwb eng Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Richard Savage, C. oth Schumer, J.M. oth Weinfeld, H. oth in Analytical Biochemistry Amsterdam : Elsevier 53(1973), 2, Seite 431-440 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:53 year:1973 number:2 pages:431-440 http://dx.doi.org/10.1016/0003-2697(73)90090-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 53 1973 2 431-440 |
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(DE-627)NLEJ184053269 (DE-599)GBVNLZ184053269 DE-627 ger DE-627 rakwb eng Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Richard Savage, C. oth Schumer, J.M. oth Weinfeld, H. oth in Analytical Biochemistry Amsterdam : Elsevier 53(1973), 2, Seite 431-440 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:53 year:1973 number:2 pages:431-440 http://dx.doi.org/10.1016/0003-2697(73)90090-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 53 1973 2 431-440 |
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(DE-627)NLEJ184053269 (DE-599)GBVNLZ184053269 DE-627 ger DE-627 rakwb eng Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Richard Savage, C. oth Schumer, J.M. oth Weinfeld, H. oth in Analytical Biochemistry Amsterdam : Elsevier 53(1973), 2, Seite 431-440 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:53 year:1973 number:2 pages:431-440 http://dx.doi.org/10.1016/0003-2697(73)90090-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 53 1973 2 431-440 |
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(DE-627)NLEJ184053269 (DE-599)GBVNLZ184053269 DE-627 ger DE-627 rakwb eng Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Richard Savage, C. oth Schumer, J.M. oth Weinfeld, H. oth in Analytical Biochemistry Amsterdam : Elsevier 53(1973), 2, Seite 431-440 (DE-627)NLEJ17685830X (DE-600)1461105-3 0003-2697 nnns volume:53 year:1973 number:2 pages:431-440 http://dx.doi.org/10.1016/0003-2697(73)90090-0 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 53 1973 2 431-440 |
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Colorimetric assay of liver and Escherichia coli aspartate transcarbamylases in the presence of 2-mercaptoethanol |
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A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. |
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A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. |
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A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. |
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