Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro
Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyalur...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1983 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Developmental Biology - Amsterdam : Elsevier, 97(1983), 1, Seite 146-153 |
| Übergeordnetes Werk: |
volume:97 ; year:1983 ; number:1 ; pages:146-153 |
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| 245 | 1 | 0 | |a Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro |
| 264 | 1 | |c 1983 | |
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| 520 | |a Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. | ||
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(DE-627)NLEJ18425910X (DE-599)GBVNLZ18425910X DE-627 ger DE-627 rakwb eng Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Merrilees, M.J. oth Sodek, J. oth Aubin, J.E. oth in Developmental Biology Amsterdam : Elsevier 97(1983), 1, Seite 146-153 (DE-627)NLEJ176859918 (DE-600)1463203-2 0012-1606 nnns volume:97 year:1983 number:1 pages:146-153 http://dx.doi.org/10.1016/0012-1606(83)90072-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 97 1983 1 146-153 |
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(DE-627)NLEJ18425910X (DE-599)GBVNLZ18425910X DE-627 ger DE-627 rakwb eng Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Merrilees, M.J. oth Sodek, J. oth Aubin, J.E. oth in Developmental Biology Amsterdam : Elsevier 97(1983), 1, Seite 146-153 (DE-627)NLEJ176859918 (DE-600)1463203-2 0012-1606 nnns volume:97 year:1983 number:1 pages:146-153 http://dx.doi.org/10.1016/0012-1606(83)90072-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 97 1983 1 146-153 |
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(DE-627)NLEJ18425910X (DE-599)GBVNLZ18425910X DE-627 ger DE-627 rakwb eng Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Merrilees, M.J. oth Sodek, J. oth Aubin, J.E. oth in Developmental Biology Amsterdam : Elsevier 97(1983), 1, Seite 146-153 (DE-627)NLEJ176859918 (DE-600)1463203-2 0012-1606 nnns volume:97 year:1983 number:1 pages:146-153 http://dx.doi.org/10.1016/0012-1606(83)90072-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 97 1983 1 146-153 |
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(DE-627)NLEJ18425910X (DE-599)GBVNLZ18425910X DE-627 ger DE-627 rakwb eng Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Merrilees, M.J. oth Sodek, J. oth Aubin, J.E. oth in Developmental Biology Amsterdam : Elsevier 97(1983), 1, Seite 146-153 (DE-627)NLEJ176859918 (DE-600)1463203-2 0012-1606 nnns volume:97 year:1983 number:1 pages:146-153 http://dx.doi.org/10.1016/0012-1606(83)90072-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 97 1983 1 146-153 |
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(DE-627)NLEJ18425910X (DE-599)GBVNLZ18425910X DE-627 ger DE-627 rakwb eng Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Merrilees, M.J. oth Sodek, J. oth Aubin, J.E. oth in Developmental Biology Amsterdam : Elsevier 97(1983), 1, Seite 146-153 (DE-627)NLEJ176859918 (DE-600)1463203-2 0012-1606 nnns volume:97 year:1983 number:1 pages:146-153 http://dx.doi.org/10.1016/0012-1606(83)90072-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 97 1983 1 146-153 |
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Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro |
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effect of cells of epithelial rests of malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro |
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Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro |
| abstract |
Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. |
| abstractGer |
Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. |
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Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ18425910X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706224611.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1983 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ18425910X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ18425910X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of cells of epithelial rests of Malassez and endothelial cells on synthesis of glycosaminoglycans by periodontal ligament fibroblasts in vitro</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1983</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Cultures of fibroblast-like cells (PLF) and epithelial rest cells (PLE) prepared from explants of porcine periodontal ligament synthesized and secreted four glycosaminoglycans (GAG) in differing proportions. The PLF produced predominantly chondroitin sulphate (>60%) with smaller amounts of hyaluronic acid (HA) (17%), dermatan sulfate (13%), and heparan sulfate (7%), whereas PLE produced predominantly HA (>80%). In coculture and under conditions of reciprocal transfer of conditioned media neither cell type affected the other's GAG synthesis. Endothelial cells (EC), however, or their conditioned growth media, were able to stimulate increased GAG synthesis, especially HA, in PLF. A similar result was obtained with smooth muscle cells (SMC) cultured in EC growth media but here again PLE were unable to stimulate GAG synthesis by SMC. These findings suggest that the spectrum of GAG found in whole ligament results both from independent production by, and from interaction between, the different cell types within the ligament. The results also provide support for a general hypothesis that loose connective tissues, which are rich in HA, are formed and maintained under the influence of epithelial, including endothelial, cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Merrilees, M.J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sodek, J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Aubin, J.E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Developmental Biology</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">97(1983), 1, Seite 146-153</subfield><subfield code="w">(DE-627)NLEJ176859918</subfield><subfield code="w">(DE-600)1463203-2</subfield><subfield code="x">0012-1606</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:97</subfield><subfield code="g">year:1983</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:146-153</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1016/0012-1606(83)90072-6</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">97</subfield><subfield code="j">1983</subfield><subfield code="e">1</subfield><subfield code="h">146-153</subfield></datafield></record></collection>
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7.4000845 |