Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat
The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1982 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Experimental Cell Research - Amsterdam : Elsevier, 140(1982), 1, Seite 111-118 |
| Übergeordnetes Werk: |
volume:140 ; year:1982 ; number:1 ; pages:111-118 |
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| 520 | |a The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. | ||
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(DE-627)NLEJ184284511 (DE-599)GBVNLZ184284511 DE-627 ger DE-627 rakwb eng Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bucci, L.R. oth Brock, W.A. oth Meistrich, M.L. oth in Experimental Cell Research Amsterdam : Elsevier 140(1982), 1, Seite 111-118 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:140 year:1982 number:1 pages:111-118 http://dx.doi.org/10.1016/0014-4827(82)90162-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 140 1982 1 111-118 |
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(DE-627)NLEJ184284511 (DE-599)GBVNLZ184284511 DE-627 ger DE-627 rakwb eng Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bucci, L.R. oth Brock, W.A. oth Meistrich, M.L. oth in Experimental Cell Research Amsterdam : Elsevier 140(1982), 1, Seite 111-118 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:140 year:1982 number:1 pages:111-118 http://dx.doi.org/10.1016/0014-4827(82)90162-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 140 1982 1 111-118 |
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(DE-627)NLEJ184284511 (DE-599)GBVNLZ184284511 DE-627 ger DE-627 rakwb eng Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bucci, L.R. oth Brock, W.A. oth Meistrich, M.L. oth in Experimental Cell Research Amsterdam : Elsevier 140(1982), 1, Seite 111-118 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:140 year:1982 number:1 pages:111-118 http://dx.doi.org/10.1016/0014-4827(82)90162-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 140 1982 1 111-118 |
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(DE-627)NLEJ184284511 (DE-599)GBVNLZ184284511 DE-627 ger DE-627 rakwb eng Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bucci, L.R. oth Brock, W.A. oth Meistrich, M.L. oth in Experimental Cell Research Amsterdam : Elsevier 140(1982), 1, Seite 111-118 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:140 year:1982 number:1 pages:111-118 http://dx.doi.org/10.1016/0014-4827(82)90162-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 140 1982 1 111-118 |
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(DE-627)NLEJ184284511 (DE-599)GBVNLZ184284511 DE-627 ger DE-627 rakwb eng Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bucci, L.R. oth Brock, W.A. oth Meistrich, M.L. oth in Experimental Cell Research Amsterdam : Elsevier 140(1982), 1, Seite 111-118 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:140 year:1982 number:1 pages:111-118 http://dx.doi.org/10.1016/0014-4827(82)90162-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 140 1982 1 111-118 |
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Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat |
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The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. |
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The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. |
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The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ184284511</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210706225020.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1982 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ184284511</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ184284511</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1982</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The distribution and synthesis of various histone 1 (H1) subfractions have been studied in purified populations of rat testis germinal cells. Rat testis H1 has been resolved by SDS polyacrylamide gel electrophoresis into four bands containing H1 subfractions H1b + H1d + H1e (H1bde), H1a, H1c, and H1t. Results show that the somatic H1 subfractions H1a, b, c, d, and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction, H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. These results show that gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bucci, L.R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brock, W.A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Meistrich, M.L.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Experimental Cell Research</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">140(1982), 1, Seite 111-118</subfield><subfield code="w">(DE-627)NLEJ177031808</subfield><subfield code="w">(DE-600)1466780-0</subfield><subfield code="x">0014-4827</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:140</subfield><subfield code="g">year:1982</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:111-118</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1016/0014-4827(82)90162-8</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">140</subfield><subfield code="j">1982</subfield><subfield code="e">1</subfield><subfield code="h">111-118</subfield></datafield></record></collection>
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