Giant pools of DNA precursors in sea urchin eggs
Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high lev...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1975 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Experimental Cell Research - Amsterdam : Elsevier, 92(1975), 1, Seite 47-56 |
| Übergeordnetes Werk: |
volume:92 ; year:1975 ; number:1 ; pages:47-56 |
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| 520 | |a Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. | ||
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(DE-627)NLEJ184366909 (DE-599)GBVNLZ184366909 DE-627 ger DE-627 rakwb eng Giant pools of DNA precursors in sea urchin eggs 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mathews, C.K. oth in Experimental Cell Research Amsterdam : Elsevier 92(1975), 1, Seite 47-56 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:92 year:1975 number:1 pages:47-56 http://dx.doi.org/10.1016/0014-4827(75)90635-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 92 1975 1 47-56 |
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(DE-627)NLEJ184366909 (DE-599)GBVNLZ184366909 DE-627 ger DE-627 rakwb eng Giant pools of DNA precursors in sea urchin eggs 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mathews, C.K. oth in Experimental Cell Research Amsterdam : Elsevier 92(1975), 1, Seite 47-56 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:92 year:1975 number:1 pages:47-56 http://dx.doi.org/10.1016/0014-4827(75)90635-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 92 1975 1 47-56 |
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(DE-627)NLEJ184366909 (DE-599)GBVNLZ184366909 DE-627 ger DE-627 rakwb eng Giant pools of DNA precursors in sea urchin eggs 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mathews, C.K. oth in Experimental Cell Research Amsterdam : Elsevier 92(1975), 1, Seite 47-56 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:92 year:1975 number:1 pages:47-56 http://dx.doi.org/10.1016/0014-4827(75)90635-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 92 1975 1 47-56 |
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(DE-627)NLEJ184366909 (DE-599)GBVNLZ184366909 DE-627 ger DE-627 rakwb eng Giant pools of DNA precursors in sea urchin eggs 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mathews, C.K. oth in Experimental Cell Research Amsterdam : Elsevier 92(1975), 1, Seite 47-56 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:92 year:1975 number:1 pages:47-56 http://dx.doi.org/10.1016/0014-4827(75)90635-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 92 1975 1 47-56 |
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(DE-627)NLEJ184366909 (DE-599)GBVNLZ184366909 DE-627 ger DE-627 rakwb eng Giant pools of DNA precursors in sea urchin eggs 1975 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mathews, C.K. oth in Experimental Cell Research Amsterdam : Elsevier 92(1975), 1, Seite 47-56 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:92 year:1975 number:1 pages:47-56 http://dx.doi.org/10.1016/0014-4827(75)90635-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 92 1975 1 47-56 |
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Giant pools of DNA precursors in sea urchin eggs |
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Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. |
| abstractGer |
Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. |
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Pools of deoxyribonucleoside 5' triphosphates were measured in eggs of the sea urchin, Strongylocentrotus purpuratus, both before and early after fertilization. The pools in unfertilized eggs are very large, amounting to between one and seven diploid nuclear equivalent units/egg. These high levels are maintained, with some fluctuations, for more than 2 h after fertilization, even in the presence of inhibitors of deoxynucleotide synthesis. This points to the existence of a large reservoir of dNTP precursors in the egg, as had been inferred from earlier studies. Exogenous radiolabeled DNA precursors, notably ^3H-thymidine, are routinely used to monitor DNA synthesis in sea urchins and other organisms. Because of the giant endogenous precursor pools and their fluctuations, plus previously described changes in deoxynucleoside transport and phosphorylation after fertilization, the relationship between thymidine labeling and DNA synthesis requires clarification. At high thymidine concentrations, as shown herein, nearly all of the DNA thymine residues appear to be derived from exogenous thymidine during the first S phase. The mechanism of this preferential utilization is not known. |
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