Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum
Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were see...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1971 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Experimental Cell Research - Amsterdam : Elsevier, 67(1971), 1, Seite 1-10 |
| Übergeordnetes Werk: |
volume:67 ; year:1971 ; number:1 ; pages:1-10 |
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NLEJ184367131 |
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| 245 | 1 | 0 | |a Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum |
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| 520 | |a Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. | ||
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| 700 | 1 | |a Lamont, M. |4 oth | |
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(DE-627)NLEJ184367131 (DE-599)GBVNLZ184367131 DE-627 ger DE-627 rakwb eng Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum 1971 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dawkins, R.L. oth Lamont, M. oth in Experimental Cell Research Amsterdam : Elsevier 67(1971), 1, Seite 1-10 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:67 year:1971 number:1 pages:1-10 http://dx.doi.org/10.1016/0014-4827(71)90614-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 67 1971 1 1-10 |
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(DE-627)NLEJ184367131 (DE-599)GBVNLZ184367131 DE-627 ger DE-627 rakwb eng Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum 1971 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dawkins, R.L. oth Lamont, M. oth in Experimental Cell Research Amsterdam : Elsevier 67(1971), 1, Seite 1-10 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:67 year:1971 number:1 pages:1-10 http://dx.doi.org/10.1016/0014-4827(71)90614-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 67 1971 1 1-10 |
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(DE-627)NLEJ184367131 (DE-599)GBVNLZ184367131 DE-627 ger DE-627 rakwb eng Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum 1971 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dawkins, R.L. oth Lamont, M. oth in Experimental Cell Research Amsterdam : Elsevier 67(1971), 1, Seite 1-10 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:67 year:1971 number:1 pages:1-10 http://dx.doi.org/10.1016/0014-4827(71)90614-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 67 1971 1 1-10 |
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(DE-627)NLEJ184367131 (DE-599)GBVNLZ184367131 DE-627 ger DE-627 rakwb eng Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum 1971 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dawkins, R.L. oth Lamont, M. oth in Experimental Cell Research Amsterdam : Elsevier 67(1971), 1, Seite 1-10 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:67 year:1971 number:1 pages:1-10 http://dx.doi.org/10.1016/0014-4827(71)90614-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 67 1971 1 1-10 |
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(DE-627)NLEJ184367131 (DE-599)GBVNLZ184367131 DE-627 ger DE-627 rakwb eng Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum 1971 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dawkins, R.L. oth Lamont, M. oth in Experimental Cell Research Amsterdam : Elsevier 67(1971), 1, Seite 1-10 (DE-627)NLEJ177031808 (DE-600)1466780-0 0014-4827 nnns volume:67 year:1971 number:1 pages:1-10 http://dx.doi.org/10.1016/0014-4827(71)90614-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 67 1971 1 1-10 |
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myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum |
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Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum |
| abstract |
Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. |
| abstractGer |
Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. |
| abstract_unstemmed |
Immunofluorescent staining of trypsinized chick muscle cultures with a guinea pig anti-rabbit-muscle serum has allowed specific identification of muscle cells at an earlier stage than has previously been reported.When filtrates of trypsinized muscle were stained rounded multinucleated cells were seen. When sequential cultures were examined it was found that these multinucleated cells developed into typical myotubes within 18 h. Mononucleated myoblasts were stained after 9 h in culture; these apparently fused and subsequently followed the well recognised pattern of myogenesis. Cross-striations were stained after only 18 h.These results indicate that both multinucleated and mononucleated cells can contribute to myogenesis after trypsinization. |
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