Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells
Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth tempera...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1973 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Biochimica et Biophysica Acta (BBA)/Biomembranes - Amsterdam : Elsevier, 298(1973), 1, Seite 39-49 |
| Übergeordnetes Werk: |
volume:298 ; year:1973 ; number:1 ; pages:39-49 |
| Links: |
|---|
| Katalog-ID: |
NLEJ185711391 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ185711391 | ||
| 003 | DE-627 | ||
| 005 | 20210707024112.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070506s1973 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ185711391 | ||
| 035 | |a (DE-599)GBVNLZ185711391 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| 264 | 1 | |c 1973 | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. | ||
| 533 | |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 | ||
| 700 | 1 | |a Wunderlich, F. |4 oth | |
| 700 | 1 | |a Speth, V. |4 oth | |
| 700 | 1 | |a Batz, W. |4 oth | |
| 700 | 1 | |a Kleinig, H. |4 oth | |
| 773 | 0 | 8 | |i in |t Biochimica et Biophysica Acta (BBA)/Biomembranes |d Amsterdam : Elsevier |g 298(1973), 1, Seite 39-49 |w (DE-627)NLEJ185706983 |w (DE-600)2209384-9 |x 0005-2736 |7 nnns |
| 773 | 1 | 8 | |g volume:298 |g year:1973 |g number:1 |g pages:39-49 |
| 856 | 4 | 0 | |u http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 |
| 912 | |a GBV_USEFLAG_H | ||
| 912 | |a ZDB-1-SDJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 298 |j 1973 |e 1 |h 39-49 | ||
| matchkey_str |
article:00052736:1973----::ebaeottayeaitefetfeprtrommrncrsrcueadataic |
|---|---|
| hierarchy_sort_str |
1973 |
| publishDate |
1973 |
| allfields |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 DE-627 ger DE-627 rakwb eng Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Speth, V. oth Batz, W. oth Kleinig, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 298(1973), 1, Seite 39-49 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:298 year:1973 number:1 pages:39-49 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 298 1973 1 39-49 |
| spelling |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 DE-627 ger DE-627 rakwb eng Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Speth, V. oth Batz, W. oth Kleinig, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 298(1973), 1, Seite 39-49 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:298 year:1973 number:1 pages:39-49 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 298 1973 1 39-49 |
| allfields_unstemmed |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 DE-627 ger DE-627 rakwb eng Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Speth, V. oth Batz, W. oth Kleinig, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 298(1973), 1, Seite 39-49 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:298 year:1973 number:1 pages:39-49 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 298 1973 1 39-49 |
| allfieldsGer |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 DE-627 ger DE-627 rakwb eng Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Speth, V. oth Batz, W. oth Kleinig, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 298(1973), 1, Seite 39-49 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:298 year:1973 number:1 pages:39-49 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 298 1973 1 39-49 |
| allfieldsSound |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 DE-627 ger DE-627 rakwb eng Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells 1973 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Speth, V. oth Batz, W. oth Kleinig, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 298(1973), 1, Seite 39-49 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:298 year:1973 number:1 pages:39-49 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 298 1973 1 39-49 |
| language |
English |
| source |
in Biochimica et Biophysica Acta (BBA)/Biomembranes 298(1973), 1, Seite 39-49 volume:298 year:1973 number:1 pages:39-49 |
| sourceStr |
in Biochimica et Biophysica Acta (BBA)/Biomembranes 298(1973), 1, Seite 39-49 volume:298 year:1973 number:1 pages:39-49 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Biochimica et Biophysica Acta (BBA)/Biomembranes |
| authorswithroles_txt_mv |
Wunderlich, F. @@oth@@ Speth, V. @@oth@@ Batz, W. @@oth@@ Kleinig, H. @@oth@@ |
| publishDateDaySort_date |
1973-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ185706983 |
| id |
NLEJ185711391 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ185711391</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707024112.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1973 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ185711391</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ185711391</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1973</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wunderlich, F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Speth, V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Batz, W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kleinig, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Biochimica et Biophysica Acta (BBA)/Biomembranes</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">298(1973), 1, Seite 39-49</subfield><subfield code="w">(DE-627)NLEJ185706983</subfield><subfield code="w">(DE-600)2209384-9</subfield><subfield code="x">0005-2736</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:298</subfield><subfield code="g">year:1973</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:39-49</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">298</subfield><subfield code="j">1973</subfield><subfield code="e">1</subfield><subfield code="h">39-49</subfield></datafield></record></collection>
|
| series2 |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ185706983 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
0005-2736 |
| topic_title |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
f w fw v s vs w b wb h k hk |
| hierarchy_parent_title |
Biochimica et Biophysica Acta (BBA)/Biomembranes |
| hierarchy_parent_id |
NLEJ185706983 |
| hierarchy_top_title |
Biochimica et Biophysica Acta (BBA)/Biomembranes |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ185706983 (DE-600)2209384-9 |
| title |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| spellingShingle |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| ctrlnum |
(DE-627)NLEJ185711391 (DE-599)GBVNLZ185711391 |
| title_full |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| journal |
Biochimica et Biophysica Acta (BBA)/Biomembranes |
| journalStr |
Biochimica et Biophysica Acta (BBA)/Biomembranes |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1973 |
| contenttype_str_mv |
zzz |
| container_start_page |
39 |
| container_volume |
298 |
| format_se |
Elektronische Aufsätze |
| title_sort |
membranes of tetrahymena - iii. the effect of temperature on membrane core structures and fatty acid composition of tetrahymena cells |
| title_auth |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| abstract |
Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. |
| abstractGer |
Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. |
| abstract_unstemmed |
Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''. |
| collection_details |
GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
| container_issue |
1 |
| title_short |
Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells |
| url |
http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2 |
| remote_bool |
true |
| author2 |
Wunderlich, F. Speth, V. Batz, W. Kleinig, H. |
| author2Str |
Wunderlich, F. Speth, V. Batz, W. Kleinig, H. |
| ppnlink |
NLEJ185706983 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth |
| up_date |
2024-07-06T04:46:45.400Z |
| _version_ |
1803803642193510400 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ185711391</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707024112.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1973 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ185711391</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ185711391</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Membranes of Tetrahymena - III. The effect of temperature on membrane core structures and fatty acid composition of Tetrahymena cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1973</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Membrane core structures as revealed by the freeze-etch electron microscopy and the fatty acid composition measured by gas-liquid chromatography have been analyzed in Tetrahymena cells exposed to low temperature for varying periods.When cells were grown to mid-log phase at the optimal growth temperature of 28 ^oC and then chilled to 10 ^oC, cell division was inhibited. However, within 16 h the cells adapted to the low temperature.Chilling effected drastic structural alterations in the cores of different membrane types (membranes of the pellicula, the alveolar sacs, the endoplasmic reticulum and the nuclei). In all cases, there was a segregation of smooth faces from particle-rich faces in the fracture planes. However, the native membrane state, i.e. like that of cells grown at 28 ^oC, reappeared when the cells adapted to the low temperature.The total lipids of Tetrahymena cells contained primarily even-numbered fatty acids ranging from C"1"2 to C"1"8, but we also detected appreciable amounts of C"2"0 acids; this has not been reported before. During the initial phase of chilling, when cell division is inhibited, about 50% of the saturated fatty acids were replaced by unsaturated fatty acids, primarily monoenoic, dienoic and trienoic acids.We conclude that the structural recovery of the membranes in chilled Tetrahymena cells is accomplished by a desaturation of membrane fatty acids. This is discussed with respect to membrane ''fluidity''.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wunderlich, F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Speth, V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Batz, W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kleinig, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Biochimica et Biophysica Acta (BBA)/Biomembranes</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">298(1973), 1, Seite 39-49</subfield><subfield code="w">(DE-627)NLEJ185706983</subfield><subfield code="w">(DE-600)2209384-9</subfield><subfield code="x">0005-2736</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:298</subfield><subfield code="g">year:1973</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:39-49</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0005-2736(73)90007-2</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">298</subfield><subfield code="j">1973</subfield><subfield code="e">1</subfield><subfield code="h">39-49</subfield></datafield></record></collection>
|
| score |
7.399371 |