Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy
1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smoo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1974 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Biochimica et Biophysica Acta (BBA)/Biomembranes - Amsterdam : Elsevier, 373(1974), 1, Seite 34-43 |
| Übergeordnetes Werk: |
volume:373 ; year:1974 ; number:1 ; pages:34-43 |
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NLEJ185717519 |
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| 245 | 1 | 0 | |a Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy |
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| 520 | |a 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. | ||
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| 700 | 1 | |a Fischer, H. |4 oth | |
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(DE-627)NLEJ185717519 (DE-599)GBVNLZ185717519 DE-627 ger DE-627 rakwb eng Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy 1974 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Wallach, D.F.H. oth Speth, V. oth Fischer, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 373(1974), 1, Seite 34-43 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:373 year:1974 number:1 pages:34-43 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 373 1974 1 34-43 |
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(DE-627)NLEJ185717519 (DE-599)GBVNLZ185717519 DE-627 ger DE-627 rakwb eng Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy 1974 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Wallach, D.F.H. oth Speth, V. oth Fischer, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 373(1974), 1, Seite 34-43 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:373 year:1974 number:1 pages:34-43 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 373 1974 1 34-43 |
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(DE-627)NLEJ185717519 (DE-599)GBVNLZ185717519 DE-627 ger DE-627 rakwb eng Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy 1974 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Wallach, D.F.H. oth Speth, V. oth Fischer, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 373(1974), 1, Seite 34-43 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:373 year:1974 number:1 pages:34-43 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 373 1974 1 34-43 |
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(DE-627)NLEJ185717519 (DE-599)GBVNLZ185717519 DE-627 ger DE-627 rakwb eng Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy 1974 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Wallach, D.F.H. oth Speth, V. oth Fischer, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 373(1974), 1, Seite 34-43 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:373 year:1974 number:1 pages:34-43 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 373 1974 1 34-43 |
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(DE-627)NLEJ185717519 (DE-599)GBVNLZ185717519 DE-627 ger DE-627 rakwb eng Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy 1974 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wunderlich, F. oth Wallach, D.F.H. oth Speth, V. oth Fischer, H. oth in Biochimica et Biophysica Acta (BBA)/Biomembranes Amsterdam : Elsevier 373(1974), 1, Seite 34-43 (DE-627)NLEJ185706983 (DE-600)2209384-9 0005-2736 nnns volume:373 year:1974 number:1 pages:34-43 http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 373 1974 1 34-43 |
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differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: a study by freeze-etch electron microscopy |
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Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy |
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1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. |
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1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. |
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1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ185717519</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707024209.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1974 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ185717519</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ185717519</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Differential effects of temperature on the nuclear and plasma membranes of lymphoid cells: A study by freeze-etch electron microscopy</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1974</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">1. We have used freeze-fracture electron microscopy to examine the effects of cooling on the core ultrastructure of the plasma and nuclear membranes of normal thymocytes and lymph node cells, as well as concanavalin A-treated thymocytes and mouse lymphoma cells.2. Chilling below 22 ^oC produces smooth areas, free of intramembranous particles on both faces of both inner and outer nuclear membranes. This effect is reversible and can be prevented by glutaraldehyde fixation.3. Plasma membranes, in contrast to the nuclear membranes, exhibit no change in freeze-fracture morphology upon cooling.4. We hypothesize that the changes observed in the nuclear membranes represent thermotropic lipid phase transitions and that such transitions either do not occur in plasma membranes or are there constrained to very small regions.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wunderlich, F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wallach, D.F.H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Speth, V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fischer, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Biochimica et Biophysica Acta (BBA)/Biomembranes</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">373(1974), 1, Seite 34-43</subfield><subfield code="w">(DE-627)NLEJ185706983</subfield><subfield code="w">(DE-600)2209384-9</subfield><subfield code="x">0005-2736</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:373</subfield><subfield code="g">year:1974</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:34-43</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0005-2736(74)90102-3</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">373</subfield><subfield code="j">1974</subfield><subfield code="e">1</subfield><subfield code="h">34-43</subfield></datafield></record></collection>
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