The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses
(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochem...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1976 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: BBA - Protein Structure - Amsterdam : Elsevier, 446(1976), 2, Seite 419-431 |
| Übergeordnetes Werk: |
volume:446 ; year:1976 ; number:2 ; pages:419-431 |
| Links: |
|---|
| Katalog-ID: |
NLEJ186045549 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ186045549 | ||
| 003 | DE-627 | ||
| 005 | 20210707033739.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070506s1976 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ186045549 | ||
| 035 | |a (DE-599)GBVNLZ186045549 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 4 | |a The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| 264 | 1 | |c 1976 | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. | ||
| 533 | |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 | ||
| 700 | 1 | |a Bhakdi, S. |4 oth | |
| 700 | 1 | |a Bjerrum, O.J. |4 oth | |
| 700 | 1 | |a Knufermann, H. |4 oth | |
| 773 | 0 | 8 | |i in |t BBA - Protein Structure |d Amsterdam : Elsevier |g 446(1976), 2, Seite 419-431 |w (DE-627)NLEJ176858709 |w (DE-600)2209544-5 |x 0005-2795 |7 nnns |
| 773 | 1 | 8 | |g volume:446 |g year:1976 |g number:2 |g pages:419-431 |
| 856 | 4 | 0 | |u http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 |
| 912 | |a GBV_USEFLAG_H | ||
| 912 | |a ZDB-1-SDJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 446 |j 1976 |e 2 |h 419-431 | ||
| matchkey_str |
article:00052795:1976----::hmjrnrnimmrnpoenfuaeyhoyepeaaiesltoad |
|---|---|
| hierarchy_sort_str |
1976 |
| publishDate |
1976 |
| allfields |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 DE-627 ger DE-627 rakwb eng The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bhakdi, S. oth Bjerrum, O.J. oth Knufermann, H. oth in BBA - Protein Structure Amsterdam : Elsevier 446(1976), 2, Seite 419-431 (DE-627)NLEJ176858709 (DE-600)2209544-5 0005-2795 nnns volume:446 year:1976 number:2 pages:419-431 http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 446 1976 2 419-431 |
| spelling |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 DE-627 ger DE-627 rakwb eng The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bhakdi, S. oth Bjerrum, O.J. oth Knufermann, H. oth in BBA - Protein Structure Amsterdam : Elsevier 446(1976), 2, Seite 419-431 (DE-627)NLEJ176858709 (DE-600)2209544-5 0005-2795 nnns volume:446 year:1976 number:2 pages:419-431 http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 446 1976 2 419-431 |
| allfields_unstemmed |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 DE-627 ger DE-627 rakwb eng The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bhakdi, S. oth Bjerrum, O.J. oth Knufermann, H. oth in BBA - Protein Structure Amsterdam : Elsevier 446(1976), 2, Seite 419-431 (DE-627)NLEJ176858709 (DE-600)2209544-5 0005-2795 nnns volume:446 year:1976 number:2 pages:419-431 http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 446 1976 2 419-431 |
| allfieldsGer |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 DE-627 ger DE-627 rakwb eng The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bhakdi, S. oth Bjerrum, O.J. oth Knufermann, H. oth in BBA - Protein Structure Amsterdam : Elsevier 446(1976), 2, Seite 419-431 (DE-627)NLEJ176858709 (DE-600)2209544-5 0005-2795 nnns volume:446 year:1976 number:2 pages:419-431 http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 446 1976 2 419-431 |
| allfieldsSound |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 DE-627 ger DE-627 rakwb eng The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier (1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Bhakdi, S. oth Bjerrum, O.J. oth Knufermann, H. oth in BBA - Protein Structure Amsterdam : Elsevier 446(1976), 2, Seite 419-431 (DE-627)NLEJ176858709 (DE-600)2209544-5 0005-2795 nnns volume:446 year:1976 number:2 pages:419-431 http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 446 1976 2 419-431 |
| language |
English |
| source |
in BBA - Protein Structure 446(1976), 2, Seite 419-431 volume:446 year:1976 number:2 pages:419-431 |
| sourceStr |
in BBA - Protein Structure 446(1976), 2, Seite 419-431 volume:446 year:1976 number:2 pages:419-431 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
BBA - Protein Structure |
| authorswithroles_txt_mv |
Bhakdi, S. @@oth@@ Bjerrum, O.J. @@oth@@ Knufermann, H. @@oth@@ |
| publishDateDaySort_date |
1976-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ176858709 |
| id |
NLEJ186045549 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186045549</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707033739.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1976 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186045549</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186045549</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1976</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bhakdi, S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bjerrum, O.J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knufermann, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">BBA - Protein Structure</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">446(1976), 2, Seite 419-431</subfield><subfield code="w">(DE-627)NLEJ176858709</subfield><subfield code="w">(DE-600)2209544-5</subfield><subfield code="x">0005-2795</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:446</subfield><subfield code="g">year:1976</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:419-431</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">446</subfield><subfield code="j">1976</subfield><subfield code="e">2</subfield><subfield code="h">419-431</subfield></datafield></record></collection>
|
| series2 |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ176858709 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
0005-2795 |
| topic_title |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
s b sb o b ob h k hk |
| hierarchy_parent_title |
BBA - Protein Structure |
| hierarchy_parent_id |
NLEJ176858709 |
| hierarchy_top_title |
BBA - Protein Structure |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ176858709 (DE-600)2209544-5 |
| title |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| spellingShingle |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| ctrlnum |
(DE-627)NLEJ186045549 (DE-599)GBVNLZ186045549 |
| title_full |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| journal |
BBA - Protein Structure |
| journalStr |
BBA - Protein Structure |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1976 |
| contenttype_str_mv |
zzz |
| container_start_page |
419 |
| container_volume |
446 |
| format_se |
Elektronische Aufsätze |
| title_sort |
major ''intrinsic'' membrane protein of human erythrocytes preparative isolation and immunoelectrophoretic analyses |
| title_auth |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| abstract |
(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. |
| abstractGer |
(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. |
| abstract_unstemmed |
(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis. |
| collection_details |
GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
| container_issue |
2 |
| title_short |
The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses |
| url |
http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8 |
| remote_bool |
true |
| author2 |
Bhakdi, S. Bjerrum, O.J. Knufermann, H. |
| author2Str |
Bhakdi, S. Bjerrum, O.J. Knufermann, H. |
| ppnlink |
NLEJ176858709 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth |
| up_date |
2024-07-06T05:50:03.377Z |
| _version_ |
1803807624663138304 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186045549</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707033739.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1976 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186045549</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186045549</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The major ''intrinsic'' membrane protein of human erythrocytes Preparative isolation and immunoelectrophoretic analyses</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1976</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">(1)|Preparative dodecyl sulfate gel electrophoresis of human erythrocyte membrane proteins has been used to isolate dodecylsulfate band 3 containing the M,N-glycoprotein and the major ''intrinsic'' membrane protein (Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971) Biochemistry 10, 2606-2617; Bretscher, M. S. (1971) J. Mol. Biol. 59, 351-357; Bretscher, M. S. (1971) Nat. New Biol. 231, 229-232 and Marchesi, V. T. and Andrews, E. P. (1972) Science 174, 1247-1248). Subsequent isoelectric focusing in polyacrylamide gels containing Triton X-100 separates these two entities and allows their simultaneous purification.(2)|The proteins thus obtained retain their antigenic properties. They are pure according to electrophoretic and immunoelectrophoretic criteria. However, crossed immunoelectrophoresis yields evidence for molecular microheterogeneity of the major ''intrinsic'' protein.(3)|Analyses utilizing crossed immunoelectrophoresis with antibodies absorbed with intact erythrocytes show that the major ''intrinsic'' protein possesses antigenic determinants on both membrane surfaces and therefore spans the erythrocyte membrane. All determinants of the M,N-glycoprotein detectable with our antibodies were found solely on the exterior membrane surface.(4)|Neither the major ''intrinsic'' membrane protein nor the major M,N-glycoprotein bound significantly to concanavalin A in crossed immunoaffinoelectrophoresis.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bhakdi, S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bjerrum, O.J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knufermann, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">BBA - Protein Structure</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">446(1976), 2, Seite 419-431</subfield><subfield code="w">(DE-627)NLEJ176858709</subfield><subfield code="w">(DE-600)2209544-5</subfield><subfield code="x">0005-2795</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:446</subfield><subfield code="g">year:1976</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:419-431</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0005-2795(76)90008-8</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">446</subfield><subfield code="j">1976</subfield><subfield code="e">2</subfield><subfield code="h">419-431</subfield></datafield></record></collection>
|
| score |
7.4010544 |