Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct
Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was...
Ausführliche Beschreibung
Gespeichert in:
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1976 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Journal of Steroid Biochemistry - Amsterdam : Elsevier, 7(1976), 11-12, Seite 1103-1108 |
| Übergeordnetes Werk: |
volume:7 ; year:1976 ; number:11-12 ; pages:1103-1108 |
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NLEJ186328273 |
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| 520 | |a Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. | ||
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(DE-627)NLEJ186328273 (DE-599)GBVNLZ186328273 DE-627 ger DE-627 rakwb eng Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Tuohimaa, P. oth Jussila, M. oth Haapala, O. oth in Journal of Steroid Biochemistry Amsterdam : Elsevier 7(1976), 11-12, Seite 1103-1108 (DE-627)NLEJ18527725X (DE-600)2198730-0 0022-4731 nnns volume:7 year:1976 number:11-12 pages:1103-1108 http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 7 1976 11-12 1103-1108 |
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(DE-627)NLEJ186328273 (DE-599)GBVNLZ186328273 DE-627 ger DE-627 rakwb eng Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Tuohimaa, P. oth Jussila, M. oth Haapala, O. oth in Journal of Steroid Biochemistry Amsterdam : Elsevier 7(1976), 11-12, Seite 1103-1108 (DE-627)NLEJ18527725X (DE-600)2198730-0 0022-4731 nnns volume:7 year:1976 number:11-12 pages:1103-1108 http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 7 1976 11-12 1103-1108 |
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(DE-627)NLEJ186328273 (DE-599)GBVNLZ186328273 DE-627 ger DE-627 rakwb eng Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Tuohimaa, P. oth Jussila, M. oth Haapala, O. oth in Journal of Steroid Biochemistry Amsterdam : Elsevier 7(1976), 11-12, Seite 1103-1108 (DE-627)NLEJ18527725X (DE-600)2198730-0 0022-4731 nnns volume:7 year:1976 number:11-12 pages:1103-1108 http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 7 1976 11-12 1103-1108 |
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(DE-627)NLEJ186328273 (DE-599)GBVNLZ186328273 DE-627 ger DE-627 rakwb eng Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Tuohimaa, P. oth Jussila, M. oth Haapala, O. oth in Journal of Steroid Biochemistry Amsterdam : Elsevier 7(1976), 11-12, Seite 1103-1108 (DE-627)NLEJ18527725X (DE-600)2198730-0 0022-4731 nnns volume:7 year:1976 number:11-12 pages:1103-1108 http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 7 1976 11-12 1103-1108 |
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(DE-627)NLEJ186328273 (DE-599)GBVNLZ186328273 DE-627 ger DE-627 rakwb eng Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct 1976 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Tuohimaa, P. oth Jussila, M. oth Haapala, O. oth in Journal of Steroid Biochemistry Amsterdam : Elsevier 7(1976), 11-12, Seite 1103-1108 (DE-627)NLEJ18527725X (DE-600)2198730-0 0022-4731 nnns volume:7 year:1976 number:11-12 pages:1103-1108 http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 7 1976 11-12 1103-1108 |
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Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct |
| abstract |
Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. |
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Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. |
| abstract_unstemmed |
Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186328273</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707042748.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1976 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186328273</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186328273</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of progesterone on HnRNA and avidin mRNA of the chick oviduct</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1976</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Nuclear pre-mRNA (HnRNA) and polysomal avidin mRNA were extracted from diethylstilbestrol and progesterone-treated immature chick oviducts. Purity of avidin antibody precipitated polysomes as well as isolated nuclei were confirmed by electron microscopy (EM). The number of perichromatin granules was counted from the oviductal epithelial cell nuclei. Form and size of RNA extracts were studied with EM. Avidin coding activity of RNA's was determined in a rabbit reticulocyte lysate system.The nuclei and polysomes of the control animals contained the same amount of avidin coding activity per 5 g of the oviduct tissue. During the progesterone induction of the avidin synthesis the activity of nuclear pre-mRNA coding for avidin decreases while the cytoplasmic avidin mRNA increases. The total avidin coding activity increases only slightly during 24 h after progesterone. There is an initial decrease in the amount of perichromatin granules followed by an increase exceeding the level of the controls 12 and 24 h after progesterone. The EM analysis of RNA extracts revealed that the average size of pre-mRNA molecules was 12000-13000 nucleotides, whereas the size of the mRNA molecules was considerably smaller, the major portion of the mRNA fraction was about 3000 nucleotides in length. The present results suggest that the cleavage and transfer of the nuclear pre-mRNA might be the critical steps of the non-transcriptional control of avidin synthesis by progesterone.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tuohimaa, P.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jussila, M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Haapala, O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Journal of Steroid Biochemistry</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">7(1976), 11-12, Seite 1103-1108</subfield><subfield code="w">(DE-627)NLEJ18527725X</subfield><subfield code="w">(DE-600)2198730-0</subfield><subfield code="x">0022-4731</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:7</subfield><subfield code="g">year:1976</subfield><subfield code="g">number:11-12</subfield><subfield code="g">pages:1103-1108</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0022-4731(76)90040-6</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">7</subfield><subfield code="j">1976</subfield><subfield code="e">11-12</subfield><subfield code="h">1103-1108</subfield></datafield></record></collection>
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