Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells
The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of an...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1989 |
|---|
| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Amsterdam : Elsevier, 213(1989), 2, Seite 125-134 |
| Übergeordnetes Werk: |
volume:213 ; year:1989 ; number:2 ; pages:125-134 |
| Links: |
|---|
| Katalog-ID: |
NLEJ186435991 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ186435991 | ||
| 003 | DE-627 | ||
| 005 | 20210707044719.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 070506s1989 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)NLEJ186435991 | ||
| 035 | |a (DE-599)GBVNLZ186435991 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 245 | 1 | 0 | |a Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| 264 | 1 | |c 1989 | |
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. | ||
| 533 | |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 | ||
| 700 | 1 | |a Burkhart, J.G. |4 oth | |
| 700 | 1 | |a Malling, H.V. |4 oth | |
| 773 | 0 | 8 | |i in |t Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |d Amsterdam : Elsevier |g 213(1989), 2, Seite 125-134 |w (DE-627)NLEJ177212004 |w (DE-600)1491099-8 |x 0027-5107 |7 nnns |
| 773 | 1 | 8 | |g volume:213 |g year:1989 |g number:2 |g pages:125-134 |
| 856 | 4 | 0 | |u http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 |
| 912 | |a GBV_USEFLAG_H | ||
| 912 | |a ZDB-1-SDJ | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 213 |j 1989 |e 2 |h 125-134 | ||
| matchkey_str |
article:00275107:1989----::uaeeiox7a3s0noprtdnohg |
|---|---|
| hierarchy_sort_str |
1989 |
| publishDate |
1989 |
| allfields |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 DE-627 ger DE-627 rakwb eng Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Burkhart, J.G. oth Malling, H.V. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 213(1989), 2, Seite 125-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:213 year:1989 number:2 pages:125-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 213 1989 2 125-134 |
| spelling |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 DE-627 ger DE-627 rakwb eng Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Burkhart, J.G. oth Malling, H.V. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 213(1989), 2, Seite 125-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:213 year:1989 number:2 pages:125-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 213 1989 2 125-134 |
| allfields_unstemmed |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 DE-627 ger DE-627 rakwb eng Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Burkhart, J.G. oth Malling, H.V. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 213(1989), 2, Seite 125-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:213 year:1989 number:2 pages:125-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 213 1989 2 125-134 |
| allfieldsGer |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 DE-627 ger DE-627 rakwb eng Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Burkhart, J.G. oth Malling, H.V. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 213(1989), 2, Seite 125-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:213 year:1989 number:2 pages:125-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 213 1989 2 125-134 |
| allfieldsSound |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 DE-627 ger DE-627 rakwb eng Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Burkhart, J.G. oth Malling, H.V. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 213(1989), 2, Seite 125-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:213 year:1989 number:2 pages:125-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 213 1989 2 125-134 |
| language |
English |
| source |
in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 213(1989), 2, Seite 125-134 volume:213 year:1989 number:2 pages:125-134 |
| sourceStr |
in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 213(1989), 2, Seite 125-134 volume:213 year:1989 number:2 pages:125-134 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |
| authorswithroles_txt_mv |
Burkhart, J.G. @@oth@@ Malling, H.V. @@oth@@ |
| publishDateDaySort_date |
1989-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ177212004 |
| id |
NLEJ186435991 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186435991</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707044719.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186435991</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186435991</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Burkhart, J.G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Malling, H.V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">213(1989), 2, Seite 125-134</subfield><subfield code="w">(DE-627)NLEJ177212004</subfield><subfield code="w">(DE-600)1491099-8</subfield><subfield code="x">0027-5107</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:213</subfield><subfield code="g">year:1989</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:125-134</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">213</subfield><subfield code="j">1989</subfield><subfield code="e">2</subfield><subfield code="h">125-134</subfield></datafield></record></collection>
|
| series2 |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ177212004 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
0027-5107 |
| topic_title |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
j b jb h m hm |
| hierarchy_parent_title |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |
| hierarchy_parent_id |
NLEJ177212004 |
| hierarchy_top_title |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ177212004 (DE-600)1491099-8 |
| title |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| spellingShingle |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| ctrlnum |
(DE-627)NLEJ186435991 (DE-599)GBVNLZ186435991 |
| title_full |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| journal |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |
| journalStr |
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1989 |
| contenttype_str_mv |
zzz |
| container_start_page |
125 |
| container_volume |
213 |
| format_se |
Elektronische Aufsätze |
| title_sort |
mutagenesis of φx174 am3 cs70 incorporated into the genome of mouse l-cells |
| title_auth |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| abstract |
The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. |
| abstractGer |
The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. |
| abstract_unstemmed |
The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells. |
| collection_details |
GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
| container_issue |
2 |
| title_short |
Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells |
| url |
http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7 |
| remote_bool |
true |
| author2 |
Burkhart, J.G. Malling, H.V. |
| author2Str |
Burkhart, J.G. Malling, H.V. |
| ppnlink |
NLEJ177212004 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth |
| up_date |
2024-07-06T06:55:11.370Z |
| _version_ |
1803811722489757696 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186435991</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707044719.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186435991</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186435991</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mutagenesis of φX174 am3 cs70 incorporated into the genome of mouse L-cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The objective of our work with φX174 has been to develop a shuttle vector that can be used comparatively in bacterial cells, different types of mammalian cells, and possibly in the various tissues of transfenic mice, with a constant mechanism for detection and analysis of mutations independent of any host-cell type. Toward that end, we have efficiently rescued ΦX174 am3 cs70 that is host-silent and stably integrated into the genome of mouse L-cells. The particular mouse L-cell line contains tandem arrays, single copies, and fragments of ΦX that, upon restriction enzyme excision, can result in 5 potentially active copies per diploid genome. The excised ΦX DNA is recovered by column chromatography, ligated, and transfected into higly competent spheroplasts. The Rescue Efficiency, defined as the number of viable phages produced out of the total number of potentially recoverable copies, is approx. 10^-^3. The Recovery Ratio, defined as the Rescue Efficiency for chromosomally-integrated phage DNA divided by the Rescue Efficiency for ΦX am3 cs70, is close to one. Mouse L-cels containing the integrated ΦX174 am3 cs70 were treated with 20 mM ethyl methanesulfonate. The reversion frequency of am3 among progeny phages rescued from treated cells was 1.4 x 10^-^5 (193 revertants in 1.4 x 10^7 phages). This is significantly higher than the 5.8 x 10^-^7 reversion frequency of am3 (7 revertants in 1.2 x 10^-^7 phages) among progeny phages rescued from untreated cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Burkhart, J.G.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Malling, H.V.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">213(1989), 2, Seite 125-134</subfield><subfield code="w">(DE-627)NLEJ177212004</subfield><subfield code="w">(DE-600)1491099-8</subfield><subfield code="x">0027-5107</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:213</subfield><subfield code="g">year:1989</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:125-134</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0027-5107(89)90143-7</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">213</subfield><subfield code="j">1989</subfield><subfield code="e">2</subfield><subfield code="h">125-134</subfield></datafield></record></collection>
|
| score |
7.402273 |