Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures
The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases...
Ausführliche Beschreibung
Gespeichert in:
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Englisch |
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1980 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Amsterdam : Elsevier, 72(1980), 1, Seite 127-134 |
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volume:72 ; year:1980 ; number:1 ; pages:127-134 |
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| 520 | |a The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. | ||
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(DE-627)NLEJ186500203 (DE-599)GBVNLZ186500203 DE-627 ger DE-627 rakwb eng Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mutchinick, O. oth Ruz, L. oth Casas, L. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 72(1980), 1, Seite 127-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:72 year:1980 number:1 pages:127-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 72 1980 1 127-134 |
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(DE-627)NLEJ186500203 (DE-599)GBVNLZ186500203 DE-627 ger DE-627 rakwb eng Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mutchinick, O. oth Ruz, L. oth Casas, L. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 72(1980), 1, Seite 127-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:72 year:1980 number:1 pages:127-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 72 1980 1 127-134 |
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(DE-627)NLEJ186500203 (DE-599)GBVNLZ186500203 DE-627 ger DE-627 rakwb eng Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mutchinick, O. oth Ruz, L. oth Casas, L. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 72(1980), 1, Seite 127-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:72 year:1980 number:1 pages:127-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 72 1980 1 127-134 |
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(DE-627)NLEJ186500203 (DE-599)GBVNLZ186500203 DE-627 ger DE-627 rakwb eng Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mutchinick, O. oth Ruz, L. oth Casas, L. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 72(1980), 1, Seite 127-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:72 year:1980 number:1 pages:127-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 72 1980 1 127-134 |
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(DE-627)NLEJ186500203 (DE-599)GBVNLZ186500203 DE-627 ger DE-627 rakwb eng Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Mutchinick, O. oth Ruz, L. oth Casas, L. oth in Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Amsterdam : Elsevier 72(1980), 1, Seite 127-134 (DE-627)NLEJ177212004 (DE-600)1491099-8 0027-5107 nnns volume:72 year:1980 number:1 pages:127-134 http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 72 1980 1 127-134 |
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time of first-generation metaphases - i. the effect of various culture media and of fetal calf serum in human lymphocyte cultures |
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Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures |
| abstract |
The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. |
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The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. |
| abstract_unstemmed |
The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186500203</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707050151.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1980 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186500203</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186500203</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Time of first-generation metaphases - I. The effect of various culture media and of fetal calf serum in human lymphocyte cultures</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1980</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The effects were studied of various culture media and of fetal calf serum (FCS) on the appropriate culture time for the collection of almost exclusively first-generation metaphases, the lymphocyte cell-cycle time, and the incubation time at which the largest proportion of second-division metaphases is present. This was accomplished by analyzing mitotic indices, and the proportion of 1st, 2nd and 3rd or subsequent division metaphases, in human lymphocyte cultures from several donors, that were sequentially harvested at 6-h intervals during the 24-72-h period, by using the fluorescence plus Giemsa technique (FPG). Diversity introduced by individual variation and culture conditions was eliminated, and almost exclusively (99.5%) first-generation metaphases were encountered when lymphocyte cultures were harvested at 36-h. At the 48-h incubation time, an average of more that 50% of second-division mitoses was observed in cultures set up with McCoy's 5a and Ham's F-10 media, but it was less than 10% when TC 199 was used. Although the cell-cycle time and the mitotic indices showed a strict correlation with the culture media used, this was not observed in relation to the presence of FCS.These findings suggest that reported inter-laboratory differences in the frequency and type of chromosomal aberrations could be greatly reduced by analyzing unequivocal first-generation metaphases in lymphocyte cultures of 36-h, or at longer culture times, identified with the FPG technique.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mutchinick, O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ruz, L.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Casas, L.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">72(1980), 1, Seite 127-134</subfield><subfield code="w">(DE-627)NLEJ177212004</subfield><subfield code="w">(DE-600)1491099-8</subfield><subfield code="x">0027-5107</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:72</subfield><subfield code="g">year:1980</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:127-134</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0027-5107(80)90228-6</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">72</subfield><subfield code="j">1980</subfield><subfield code="e">1</subfield><subfield code="h">127-134</subfield></datafield></record></collection>
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