Age-related changes in the galactose recognition system in rat liver cells
In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm...
Ausführliche Beschreibung
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Mechanisms of Ageing and Development - Amsterdam : Elsevier, 50(1989), 1, Seite 57-69 |
| Übergeordnetes Werk: |
volume:50 ; year:1989 ; number:1 ; pages:57-69 |
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| 520 | |a In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. | ||
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(DE-627)NLEJ186555318 (DE-599)GBVNLZ186555318 DE-627 ger DE-627 rakwb eng Age-related changes in the galactose recognition system in rat liver cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dini, L. oth Conti Devirgiliis, L. oth in Mechanisms of Ageing and Development Amsterdam : Elsevier 50(1989), 1, Seite 57-69 (DE-627)NLEJ176932682 (DE-600)1502520-2 0047-6374 nnns volume:50 year:1989 number:1 pages:57-69 http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 50 1989 1 57-69 |
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(DE-627)NLEJ186555318 (DE-599)GBVNLZ186555318 DE-627 ger DE-627 rakwb eng Age-related changes in the galactose recognition system in rat liver cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dini, L. oth Conti Devirgiliis, L. oth in Mechanisms of Ageing and Development Amsterdam : Elsevier 50(1989), 1, Seite 57-69 (DE-627)NLEJ176932682 (DE-600)1502520-2 0047-6374 nnns volume:50 year:1989 number:1 pages:57-69 http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 50 1989 1 57-69 |
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(DE-627)NLEJ186555318 (DE-599)GBVNLZ186555318 DE-627 ger DE-627 rakwb eng Age-related changes in the galactose recognition system in rat liver cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dini, L. oth Conti Devirgiliis, L. oth in Mechanisms of Ageing and Development Amsterdam : Elsevier 50(1989), 1, Seite 57-69 (DE-627)NLEJ176932682 (DE-600)1502520-2 0047-6374 nnns volume:50 year:1989 number:1 pages:57-69 http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 50 1989 1 57-69 |
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(DE-627)NLEJ186555318 (DE-599)GBVNLZ186555318 DE-627 ger DE-627 rakwb eng Age-related changes in the galactose recognition system in rat liver cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dini, L. oth Conti Devirgiliis, L. oth in Mechanisms of Ageing and Development Amsterdam : Elsevier 50(1989), 1, Seite 57-69 (DE-627)NLEJ176932682 (DE-600)1502520-2 0047-6374 nnns volume:50 year:1989 number:1 pages:57-69 http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 50 1989 1 57-69 |
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(DE-627)NLEJ186555318 (DE-599)GBVNLZ186555318 DE-627 ger DE-627 rakwb eng Age-related changes in the galactose recognition system in rat liver cells 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Dini, L. oth Conti Devirgiliis, L. oth in Mechanisms of Ageing and Development Amsterdam : Elsevier 50(1989), 1, Seite 57-69 (DE-627)NLEJ176932682 (DE-600)1502520-2 0047-6374 nnns volume:50 year:1989 number:1 pages:57-69 http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 50 1989 1 57-69 |
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Age-related changes in the galactose recognition system in rat liver cells |
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In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. |
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In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. |
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In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186555318</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707051519.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1989 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186555318</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186555318</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Age-related changes in the galactose recognition system in rat liver cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1989</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">In the present report, the galactose recognition system in 3- and 24-month-old rat livers has been studied with in vitro and with in situ binding experiments and in vivo ligand uptake. The galactose-specific receptors were visualized by using colloidal gold particles of different sizes (5 nm, 17 nm and 50 nm mean diameter), coated with lactosylated bovine serum albumin (LacBSA) as electron dense ligands.The data show that aging affects the expression of galactose-specific receptors and the rate of endocytosis. In the in vitro and in situ experiments hepatocytes and liver macrophages from old rats on the plasma membrane express a decreased number of binding sites with respect to those present on the adult rat liver cells. Approximately 80% of the total number of liver macrophages from aged rats show a binding distribution which is very different from the typical clustered receptor arrangement: the binding sites appear as single or small clusters of gold granules. As a direct consequence of the altered pattern of the receptor distribution, the capacity of liver macrophages from 24-month-old rats to internalize the larger ligands (17 nm and 50 nm) is decreased, as compared with adult rats.Aging, therefore, influences the galactose recognition system in two ways: (i) by decreasing the number of binding sites expressed on the liver cell surfaces; and (ii) by modifying the receptor distribution on liver macrophages and consequently affecting the internalization of galactose exposing particles.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dini, L.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Conti Devirgiliis, L.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Mechanisms of Ageing and Development</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">50(1989), 1, Seite 57-69</subfield><subfield code="w">(DE-627)NLEJ176932682</subfield><subfield code="w">(DE-600)1502520-2</subfield><subfield code="x">0047-6374</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:50</subfield><subfield code="g">year:1989</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:57-69</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0047-6374(89)90059-6</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">50</subfield><subfield code="j">1989</subfield><subfield code="e">1</subfield><subfield code="h">57-69</subfield></datafield></record></collection>
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7.401991 |