Localization of amplified c-myc and n-myc in small cell lung cancer cell lines
In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-m...
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Gespeichert in:
| Autor*in: |
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1989 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Cancer Genetics and Cytogenetics - Amsterdam : Elsevier, 38(1989), 1, Seite 1-8 |
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volume:38 ; year:1989 ; number:1 ; pages:1-8 |
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| 520 | |a In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. | ||
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(DE-627)NLEJ186659245 (DE-599)GBVNLZ186659245 DE-627 ger DE-627 rakwb eng Localization of amplified c-myc and n-myc in small cell lung cancer cell lines 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 van der Hout, A.H. oth Kok, K. oth van der Veen, A.Y. oth Osinga, J. oth de Leij, L.F.M.H. oth Buys, C.H.C.M. oth in Cancer Genetics and Cytogenetics Amsterdam : Elsevier 38(1989), 1, Seite 1-8 (DE-627)NLEJ186641591 (DE-600)2004205-X 0165-4608 nnns volume:38 year:1989 number:1 pages:1-8 http://linkinghub.elsevier.com/retrieve/pii/0165-4608(89)90158-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1989 1 1-8 |
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(DE-627)NLEJ186659245 (DE-599)GBVNLZ186659245 DE-627 ger DE-627 rakwb eng Localization of amplified c-myc and n-myc in small cell lung cancer cell lines 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 van der Hout, A.H. oth Kok, K. oth van der Veen, A.Y. oth Osinga, J. oth de Leij, L.F.M.H. oth Buys, C.H.C.M. oth in Cancer Genetics and Cytogenetics Amsterdam : Elsevier 38(1989), 1, Seite 1-8 (DE-627)NLEJ186641591 (DE-600)2004205-X 0165-4608 nnns volume:38 year:1989 number:1 pages:1-8 http://linkinghub.elsevier.com/retrieve/pii/0165-4608(89)90158-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1989 1 1-8 |
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(DE-627)NLEJ186659245 (DE-599)GBVNLZ186659245 DE-627 ger DE-627 rakwb eng Localization of amplified c-myc and n-myc in small cell lung cancer cell lines 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 van der Hout, A.H. oth Kok, K. oth van der Veen, A.Y. oth Osinga, J. oth de Leij, L.F.M.H. oth Buys, C.H.C.M. oth in Cancer Genetics and Cytogenetics Amsterdam : Elsevier 38(1989), 1, Seite 1-8 (DE-627)NLEJ186641591 (DE-600)2004205-X 0165-4608 nnns volume:38 year:1989 number:1 pages:1-8 http://linkinghub.elsevier.com/retrieve/pii/0165-4608(89)90158-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1989 1 1-8 |
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(DE-627)NLEJ186659245 (DE-599)GBVNLZ186659245 DE-627 ger DE-627 rakwb eng Localization of amplified c-myc and n-myc in small cell lung cancer cell lines 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 van der Hout, A.H. oth Kok, K. oth van der Veen, A.Y. oth Osinga, J. oth de Leij, L.F.M.H. oth Buys, C.H.C.M. oth in Cancer Genetics and Cytogenetics Amsterdam : Elsevier 38(1989), 1, Seite 1-8 (DE-627)NLEJ186641591 (DE-600)2004205-X 0165-4608 nnns volume:38 year:1989 number:1 pages:1-8 http://linkinghub.elsevier.com/retrieve/pii/0165-4608(89)90158-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1989 1 1-8 |
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(DE-627)NLEJ186659245 (DE-599)GBVNLZ186659245 DE-627 ger DE-627 rakwb eng Localization of amplified c-myc and n-myc in small cell lung cancer cell lines 1989 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 van der Hout, A.H. oth Kok, K. oth van der Veen, A.Y. oth Osinga, J. oth de Leij, L.F.M.H. oth Buys, C.H.C.M. oth in Cancer Genetics and Cytogenetics Amsterdam : Elsevier 38(1989), 1, Seite 1-8 (DE-627)NLEJ186641591 (DE-600)2004205-X 0165-4608 nnns volume:38 year:1989 number:1 pages:1-8 http://linkinghub.elsevier.com/retrieve/pii/0165-4608(89)90158-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1989 1 1-8 |
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localization of amplified c-myc and n-myc in small cell lung cancer cell lines |
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Localization of amplified c-myc and n-myc in small cell lung cancer cell lines |
| abstract |
In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. |
| abstractGer |
In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. |
| abstract_unstemmed |
In this study 12 small cell lung cancer cell lines were tested for amplification of myc oncogenes, the location of amplified sequences, and the possible correlation between number of dmin and degree of amplification in dmin-containing lines. C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. Apparently, the population of dmin in this cell was heterogeneous and amplified myc genes were only present on a subpopulation. |
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van der Hout, A.H. Kok, K. van der Veen, A.Y. Osinga, J. de Leij, L.F.M.H. Buys, C.H.C.M. |
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van der Hout, A.H. Kok, K. van der Veen, A.Y. Osinga, J. de Leij, L.F.M.H. Buys, C.H.C.M. |
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C-myc appeared to be amplified in four cell lines, and N-myc amplification was detected in two cell lines. No amplification of L-myc was found. The degree of amplification in the different cell lines varied between 20x and 100x. The cell lines with myc amplification appeared to contain numerous dmin, although in one cell line they occurred in only 10% of the cells. The other cells in this line contained a homogeneously staining region (HSR). In situ hybridization was carried out to find the location of the amplification. In four cell lines the amplified myc genes were found to be located on the dmin. In the cell line with the HSR in most cells and dmin in a minority of its cells, amplification was found both at the HSR and on the dmin. In one cell line the myc sequences seemed to be dispersed through the genome. The ratio between the average number of dmin per cell and the degree of amplification did not vary considerably between the cell lines, with one exception. In that cell line the number of dmin exceeded the number of myc sequences by about one order of magnitude. 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