Induction of adipose S-100 protein release by free fatty acids in adipocytes
The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/...
Ausführliche Beschreibung
Gespeichert in:
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E-Artikel |
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Englisch |
| Erschienen: |
1986 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Biochimica et Biophysica Acta (BBA)/Molecular Cell Research - Amsterdam : Elsevier, 889(1986), 1, Seite 84-90 |
| Übergeordnetes Werk: |
volume:889 ; year:1986 ; number:1 ; pages:84-90 |
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| 520 | |a The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. | ||
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(DE-627)NLEJ186868421 (DE-599)GBVNLZ186868421 DE-627 ger DE-627 rakwb eng Induction of adipose S-100 protein release by free fatty acids in adipocytes 1986 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Suzuki, F. oth Kato, K. oth in Biochimica et Biophysica Acta (BBA)/Molecular Cell Research Amsterdam : Elsevier 889(1986), 1, Seite 84-90 (DE-627)NLEJ186863756 (DE-600)2209512-3 0167-4889 nnns volume:889 year:1986 number:1 pages:84-90 http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 889 1986 1 84-90 |
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(DE-627)NLEJ186868421 (DE-599)GBVNLZ186868421 DE-627 ger DE-627 rakwb eng Induction of adipose S-100 protein release by free fatty acids in adipocytes 1986 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Suzuki, F. oth Kato, K. oth in Biochimica et Biophysica Acta (BBA)/Molecular Cell Research Amsterdam : Elsevier 889(1986), 1, Seite 84-90 (DE-627)NLEJ186863756 (DE-600)2209512-3 0167-4889 nnns volume:889 year:1986 number:1 pages:84-90 http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 889 1986 1 84-90 |
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(DE-627)NLEJ186868421 (DE-599)GBVNLZ186868421 DE-627 ger DE-627 rakwb eng Induction of adipose S-100 protein release by free fatty acids in adipocytes 1986 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Suzuki, F. oth Kato, K. oth in Biochimica et Biophysica Acta (BBA)/Molecular Cell Research Amsterdam : Elsevier 889(1986), 1, Seite 84-90 (DE-627)NLEJ186863756 (DE-600)2209512-3 0167-4889 nnns volume:889 year:1986 number:1 pages:84-90 http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 889 1986 1 84-90 |
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(DE-627)NLEJ186868421 (DE-599)GBVNLZ186868421 DE-627 ger DE-627 rakwb eng Induction of adipose S-100 protein release by free fatty acids in adipocytes 1986 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Suzuki, F. oth Kato, K. oth in Biochimica et Biophysica Acta (BBA)/Molecular Cell Research Amsterdam : Elsevier 889(1986), 1, Seite 84-90 (DE-627)NLEJ186863756 (DE-600)2209512-3 0167-4889 nnns volume:889 year:1986 number:1 pages:84-90 http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 889 1986 1 84-90 |
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(DE-627)NLEJ186868421 (DE-599)GBVNLZ186868421 DE-627 ger DE-627 rakwb eng Induction of adipose S-100 protein release by free fatty acids in adipocytes 1986 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Suzuki, F. oth Kato, K. oth in Biochimica et Biophysica Acta (BBA)/Molecular Cell Research Amsterdam : Elsevier 889(1986), 1, Seite 84-90 (DE-627)NLEJ186863756 (DE-600)2209512-3 0167-4889 nnns volume:889 year:1986 number:1 pages:84-90 http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 889 1986 1 84-90 |
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induction of adipose s-100 protein release by free fatty acids in adipocytes |
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Induction of adipose S-100 protein release by free fatty acids in adipocytes |
| abstract |
The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. |
| abstractGer |
The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. |
| abstract_unstemmed |
The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ186868421</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707061701.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1986 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ186868421</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ186868421</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Induction of adipose S-100 protein release by free fatty acids in adipocytes</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1986</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The mechanism of S-100 protein release from adipocytes, which is apparently coupled with lipolytic activity, was investigated in vitro using rat epididymal fat pads. The S-100 protein release was increased severalfold by 10 μM epinephrine in the medium containing a low concentration (less than 5 mg/ml) of albumin, but the release was enhanced only slightly when the medium contained a high concentration (more than 20 mg/ml) of albumin. On the other hand, the maximum rate of free fatty acid release measured simultaneously was observed in medium containing more than 20 mg/ml albumin. The rate of S-100 protein release was found to be closely related to the concentrations of both albumin added to the incubation medium and fatty acids released into it, and the rate was increased under conditions wherein the molar ratio of fatty acid/albumin was greater than 6. The S-100 protein release from fat pads was also enhanced solely by the addition of an excess amount (6 mM) of palmitic acid or oleic acid. The basal release of S-100 protein at a high concentration of albumin in the fat pads of diabetic or long-term starved rats, in which the fatty acid level in adipocytes is known to be enhanced, was about 7- and 2-fold higher, respectively, than that of control fed rats. These results suggest that S-100 protein in adipocytes is released under conditions in which the fatty acids being produced are not released promptly and are accumulated in the cells.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Suzuki, F.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kato, K.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Biochimica et Biophysica Acta (BBA)/Molecular Cell Research</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">889(1986), 1, Seite 84-90</subfield><subfield code="w">(DE-627)NLEJ186863756</subfield><subfield code="w">(DE-600)2209512-3</subfield><subfield code="x">0167-4889</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:889</subfield><subfield code="g">year:1986</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:84-90</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0167-4889(86)90011-X</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">889</subfield><subfield code="j">1986</subfield><subfield code="e">1</subfield><subfield code="h">84-90</subfield></datafield></record></collection>
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