A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells
Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogena...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1992 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Biochimie - Amsterdam : Elsevier, 74(1992), 9-10, Seite 941-948 |
| Übergeordnetes Werk: |
volume:74 ; year:1992 ; number:9-10 ; pages:941-948 |
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(DE-627)NLEJ186927401 (DE-599)GBVNLZ186927401 DE-627 ger DE-627 rakwb eng A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Brand, A. oth Engelmann, J. oth Leibfritz, D. oth in Biochimie Amsterdam : Elsevier 74(1992), 9-10, Seite 941-948 (DE-627)NLEJ177262761 (DE-600)2011725-5 0300-9084 nnns volume:74 year:1992 number:9-10 pages:941-948 http://linkinghub.elsevier.com/retrieve/pii/0300-9084(92)90078-S GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 74 1992 9-10 941-948 |
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(DE-627)NLEJ186927401 (DE-599)GBVNLZ186927401 DE-627 ger DE-627 rakwb eng A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Brand, A. oth Engelmann, J. oth Leibfritz, D. oth in Biochimie Amsterdam : Elsevier 74(1992), 9-10, Seite 941-948 (DE-627)NLEJ177262761 (DE-600)2011725-5 0300-9084 nnns volume:74 year:1992 number:9-10 pages:941-948 http://linkinghub.elsevier.com/retrieve/pii/0300-9084(92)90078-S GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 74 1992 9-10 941-948 |
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(DE-627)NLEJ186927401 (DE-599)GBVNLZ186927401 DE-627 ger DE-627 rakwb eng A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Brand, A. oth Engelmann, J. oth Leibfritz, D. oth in Biochimie Amsterdam : Elsevier 74(1992), 9-10, Seite 941-948 (DE-627)NLEJ177262761 (DE-600)2011725-5 0300-9084 nnns volume:74 year:1992 number:9-10 pages:941-948 http://linkinghub.elsevier.com/retrieve/pii/0300-9084(92)90078-S GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 74 1992 9-10 941-948 |
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(DE-627)NLEJ186927401 (DE-599)GBVNLZ186927401 DE-627 ger DE-627 rakwb eng A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Brand, A. oth Engelmann, J. oth Leibfritz, D. oth in Biochimie Amsterdam : Elsevier 74(1992), 9-10, Seite 941-948 (DE-627)NLEJ177262761 (DE-600)2011725-5 0300-9084 nnns volume:74 year:1992 number:9-10 pages:941-948 http://linkinghub.elsevier.com/retrieve/pii/0300-9084(92)90078-S GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 74 1992 9-10 941-948 |
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(DE-627)NLEJ186927401 (DE-599)GBVNLZ186927401 DE-627 ger DE-627 rakwb eng A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Brand, A. oth Engelmann, J. oth Leibfritz, D. oth in Biochimie Amsterdam : Elsevier 74(1992), 9-10, Seite 941-948 (DE-627)NLEJ177262761 (DE-600)2011725-5 0300-9084 nnns volume:74 year:1992 number:9-10 pages:941-948 http://linkinghub.elsevier.com/retrieve/pii/0300-9084(92)90078-S GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 74 1992 9-10 941-948 |
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A ^1^3C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells |
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Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. |
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Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. |
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Two tumor cell lines (C6 glioma and N1E-115 neuroblastoma), primary glia and primary neurons (from rat) were incubated with 2-^1^3C-pyruvate and 3-^1^3C-pyruvate in culture dishes. ^1^3C NMR spectra of the cell extracts were used to determine the ratio of pyruvate carboxylase to pyruvate dehydrogenase activity. Pyruvate carboxylase activity was found higher in primary glia cells than in neurons. Glial cells synthesized more amino acids, ie, their TCA cycle was used to a larger extent for biosynthesis than is the case of neurons, where it is preferentially used for the energy metabolism. |
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