Isolation and characterisation of a mast cell degranulating substance from Ascaris suum
1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The m...
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Englisch |
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1972 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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in: Biochimica et Biophysica Acta (BBA)/General Subjects - Amsterdam : Elsevier, 261(1972), 1, Seite 245-257 |
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volume:261 ; year:1972 ; number:1 ; pages:245-257 |
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| 520 | |a 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. | ||
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(DE-627)NLEJ187103720 (DE-599)GBVNLZ187103720 DE-627 ger DE-627 rakwb eng Isolation and characterisation of a mast cell degranulating substance from Ascaris suum 1972 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Thompson, A.R. oth in Biochimica et Biophysica Acta (BBA)/General Subjects Amsterdam : Elsevier 261(1972), 1, Seite 245-257 (DE-627)NLEJ177008172 (DE-600)2209617-6 0304-4165 nnns volume:261 year:1972 number:1 pages:245-257 http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 261 1972 1 245-257 |
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(DE-627)NLEJ187103720 (DE-599)GBVNLZ187103720 DE-627 ger DE-627 rakwb eng Isolation and characterisation of a mast cell degranulating substance from Ascaris suum 1972 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Thompson, A.R. oth in Biochimica et Biophysica Acta (BBA)/General Subjects Amsterdam : Elsevier 261(1972), 1, Seite 245-257 (DE-627)NLEJ177008172 (DE-600)2209617-6 0304-4165 nnns volume:261 year:1972 number:1 pages:245-257 http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 261 1972 1 245-257 |
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(DE-627)NLEJ187103720 (DE-599)GBVNLZ187103720 DE-627 ger DE-627 rakwb eng Isolation and characterisation of a mast cell degranulating substance from Ascaris suum 1972 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Thompson, A.R. oth in Biochimica et Biophysica Acta (BBA)/General Subjects Amsterdam : Elsevier 261(1972), 1, Seite 245-257 (DE-627)NLEJ177008172 (DE-600)2209617-6 0304-4165 nnns volume:261 year:1972 number:1 pages:245-257 http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 261 1972 1 245-257 |
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(DE-627)NLEJ187103720 (DE-599)GBVNLZ187103720 DE-627 ger DE-627 rakwb eng Isolation and characterisation of a mast cell degranulating substance from Ascaris suum 1972 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Thompson, A.R. oth in Biochimica et Biophysica Acta (BBA)/General Subjects Amsterdam : Elsevier 261(1972), 1, Seite 245-257 (DE-627)NLEJ177008172 (DE-600)2209617-6 0304-4165 nnns volume:261 year:1972 number:1 pages:245-257 http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 261 1972 1 245-257 |
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(DE-627)NLEJ187103720 (DE-599)GBVNLZ187103720 DE-627 ger DE-627 rakwb eng Isolation and characterisation of a mast cell degranulating substance from Ascaris suum 1972 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier 1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Thompson, A.R. oth in Biochimica et Biophysica Acta (BBA)/General Subjects Amsterdam : Elsevier 261(1972), 1, Seite 245-257 (DE-627)NLEJ177008172 (DE-600)2209617-6 0304-4165 nnns volume:261 year:1972 number:1 pages:245-257 http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 261 1972 1 245-257 |
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During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. 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Isolation and characterisation of a mast cell degranulating substance from Ascaris suum |
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1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. |
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1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. |
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1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ187103720</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707065247.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1972 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ187103720</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ187103720</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation and characterisation of a mast cell degranulating substance from Ascaris suum</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1972</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">1. During investigations of allergic phenomena associated with parasitic infestations, a substance which induces degranulation of mast cells has been isolated from the body fluid of Ascaris suum. It has been assayed by its ability to cause degranulation of rat peritoneal mast cells in vitro.2. The mast cell factor has reasonable stability to heat. To retain mast cell factor activity during isolation and storage of the various preparations, it was essential to prevent aggregation of the mast cell factor, presumably resulting from oxidation of sulphydryl groups. Aggregation was prevented by incorporation in the buffers of 2-mercaptoethanol and dithiothreitol. Precautions were also necessary to avoid loss of activity during concentration of fractions particularly because the molecular size of mast cell factor barely permitted its retention by the usual dialysis membranes.3. The scheme adopted for purification of mast cell factor involved successive chromatographic fractionations on DEAE-cellulose, Sephadex G-75 and SE-Sephadex. The product gave a single band on polyacrylamide-gel electrophoresis in gradient and 7% gels, as well as in gels containing sodium dodecyl sulphate. In the last system proteins were first reduced and unfolded; by comparison in dodecyl sulfate gels of the mobility of mast cell factor with that of reference proteins, mast cell factor was estimated to have a molecular weight of 8800.4. The sedimentation-velocity pattern showed a single peak with an s value of 1.07, a result corresponding to a molecular weight of the order of 8600. Amino acid analysis indicated a molecular weight of 8937. The above evidence collectively suggests that the isolated preparation of mast cell factor is substantially homogeneous.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Thompson, A.R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Biochimica et Biophysica Acta (BBA)/General Subjects</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">261(1972), 1, Seite 245-257</subfield><subfield code="w">(DE-627)NLEJ177008172</subfield><subfield code="w">(DE-600)2209617-6</subfield><subfield code="x">0304-4165</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:261</subfield><subfield code="g">year:1972</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:245-257</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0304-4165(72)90335-2</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">261</subfield><subfield code="j">1972</subfield><subfield code="e">1</subfield><subfield code="h">245-257</subfield></datafield></record></collection>
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7.402009 |