Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus
The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasm...
Ausführliche Beschreibung
Gespeichert in:
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1983 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Gene - Amsterdam : Elsevier, 26(1983), 1, Seite 19-24 |
| Übergeordnetes Werk: |
volume:26 ; year:1983 ; number:1 ; pages:19-24 |
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| 245 | 1 | 0 | |a Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus |
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| 520 | |a The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. | ||
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| 700 | 1 | |a Scherneck, S. |4 oth | |
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(DE-627)NLEJ187297029 (DE-599)GBVNLZ187297029 DE-627 ger DE-627 rakwb eng Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Krause, H. oth Garajev, M.M. oth Geissler, E. oth Platzer, M. oth Scherneck, S. oth Zimmermann, W. oth in Gene Amsterdam : Elsevier 26(1983), 1, Seite 19-24 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:26 year:1983 number:1 pages:19-24 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 26 1983 1 19-24 |
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(DE-627)NLEJ187297029 (DE-599)GBVNLZ187297029 DE-627 ger DE-627 rakwb eng Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Krause, H. oth Garajev, M.M. oth Geissler, E. oth Platzer, M. oth Scherneck, S. oth Zimmermann, W. oth in Gene Amsterdam : Elsevier 26(1983), 1, Seite 19-24 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:26 year:1983 number:1 pages:19-24 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 26 1983 1 19-24 |
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(DE-627)NLEJ187297029 (DE-599)GBVNLZ187297029 DE-627 ger DE-627 rakwb eng Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Krause, H. oth Garajev, M.M. oth Geissler, E. oth Platzer, M. oth Scherneck, S. oth Zimmermann, W. oth in Gene Amsterdam : Elsevier 26(1983), 1, Seite 19-24 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:26 year:1983 number:1 pages:19-24 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 26 1983 1 19-24 |
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(DE-627)NLEJ187297029 (DE-599)GBVNLZ187297029 DE-627 ger DE-627 rakwb eng Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Krause, H. oth Garajev, M.M. oth Geissler, E. oth Platzer, M. oth Scherneck, S. oth Zimmermann, W. oth in Gene Amsterdam : Elsevier 26(1983), 1, Seite 19-24 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:26 year:1983 number:1 pages:19-24 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 26 1983 1 19-24 |
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(DE-627)NLEJ187297029 (DE-599)GBVNLZ187297029 DE-627 ger DE-627 rakwb eng Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus 1983 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Krause, H. oth Garajev, M.M. oth Geissler, E. oth Platzer, M. oth Scherneck, S. oth Zimmermann, W. oth in Gene Amsterdam : Elsevier 26(1983), 1, Seite 19-24 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:26 year:1983 number:1 pages:19-24 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 26 1983 1 19-24 |
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Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus |
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The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. |
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The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. |
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The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ187297029</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707072050.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1983 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ187297029</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ187297029</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Studies on the SV40-like papovavirus SV40-GBM II. Molecular cloning in Escherichia coli of variant DNA molecules from glioblastoma-derived virus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1983</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The complete DNA genomes of the SV40-like GBM virus (GBM1), isolated from a human glioblastoma multiforme, and of two discrete classes of GBM DNA molecules that appear following three passages in CV-1 monkey cells at low multiplicities (GBM3-H and GBM3-L), were cloned in Escherichia coli using plasmid vector pBR322. The cloned viral DNAs were characterized (i) by digestion of the chimeric plasmid DNAs with various restriction enzymes followed by comparison of their electrophoretic mobilities in agarose with that of similarly digested uncloned DNA, (ii) by hybridization of digested chimeric plasmid DNAs to ^3^2P-labeled uncloned GBM DNA, and (iii) by electron microscopy.In restriction enzyme analysis the cloned GBM DNAs showed the same cleavage pattern as the uncloned DNAs, indicating that no major insertions or deletions were present. The electrophoretic data were confirmed by electron microscopic heteroduplex analysis.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Krause, H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Garajev, M.M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Geissler, E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Platzer, M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Scherneck, S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zimmermann, W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Gene</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">26(1983), 1, Seite 19-24</subfield><subfield code="w">(DE-627)NLEJ177212578</subfield><subfield code="w">(DE-600)1491012-3</subfield><subfield code="x">0378-1119</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:26</subfield><subfield code="g">year:1983</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:19-24</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0378-1119(83)90032-X</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">26</subfield><subfield code="j">1983</subfield><subfield code="e">1</subfield><subfield code="h">19-24</subfield></datafield></record></collection>
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