Cloning and physical mapping of the glnA gene of Escberichia coli K-12
We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases B...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1980 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Gene - Amsterdam : Elsevier, 11(1980), 3-4, Seite 239-251 |
| Übergeordnetes Werk: |
volume:11 ; year:1980 ; number:3-4 ; pages:239-251 |
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| 245 | 1 | 0 | |a Cloning and physical mapping of the glnA gene of Escberichia coli K-12 |
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| 520 | |a We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. | ||
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(DE-627)NLEJ187323046 (DE-599)GBVNLZ187323046 DE-627 ger DE-627 rakwb eng Cloning and physical mapping of the glnA gene of Escberichia coli K-12 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Covarrubias, A.A. oth Rocha, M. oth Bolivar, F. oth Bastarrachea, F. oth in Gene Amsterdam : Elsevier 11(1980), 3-4, Seite 239-251 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:11 year:1980 number:3-4 pages:239-251 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(80)90064-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 11 1980 3-4 239-251 |
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(DE-627)NLEJ187323046 (DE-599)GBVNLZ187323046 DE-627 ger DE-627 rakwb eng Cloning and physical mapping of the glnA gene of Escberichia coli K-12 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Covarrubias, A.A. oth Rocha, M. oth Bolivar, F. oth Bastarrachea, F. oth in Gene Amsterdam : Elsevier 11(1980), 3-4, Seite 239-251 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:11 year:1980 number:3-4 pages:239-251 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(80)90064-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 11 1980 3-4 239-251 |
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(DE-627)NLEJ187323046 (DE-599)GBVNLZ187323046 DE-627 ger DE-627 rakwb eng Cloning and physical mapping of the glnA gene of Escberichia coli K-12 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Covarrubias, A.A. oth Rocha, M. oth Bolivar, F. oth Bastarrachea, F. oth in Gene Amsterdam : Elsevier 11(1980), 3-4, Seite 239-251 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:11 year:1980 number:3-4 pages:239-251 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(80)90064-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 11 1980 3-4 239-251 |
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(DE-627)NLEJ187323046 (DE-599)GBVNLZ187323046 DE-627 ger DE-627 rakwb eng Cloning and physical mapping of the glnA gene of Escberichia coli K-12 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Covarrubias, A.A. oth Rocha, M. oth Bolivar, F. oth Bastarrachea, F. oth in Gene Amsterdam : Elsevier 11(1980), 3-4, Seite 239-251 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:11 year:1980 number:3-4 pages:239-251 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(80)90064-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 11 1980 3-4 239-251 |
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(DE-627)NLEJ187323046 (DE-599)GBVNLZ187323046 DE-627 ger DE-627 rakwb eng Cloning and physical mapping of the glnA gene of Escberichia coli K-12 1980 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Covarrubias, A.A. oth Rocha, M. oth Bolivar, F. oth Bastarrachea, F. oth in Gene Amsterdam : Elsevier 11(1980), 3-4, Seite 239-251 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:11 year:1980 number:3-4 pages:239-251 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(80)90064-5 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 11 1980 3-4 239-251 |
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cloning and physical mapping of the glna gene of escberichia coli k-12 |
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Cloning and physical mapping of the glnA gene of Escberichia coli K-12 |
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We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. |
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We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. |
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We have isolated part of the glnA region of Escherichia coli K-12 as a 6.4 Md DNA fragment on the ColE1 hybrid plasmid pACR1. DNA fragments from pACR1 obtained by cleavage with certain restriction endonucleases were subcloned into the pBR322 cloning vehicle. Recognition sites for the endonucleases BamHI, SmaI, BglII, and EcoRI were localized inside the glnA gene sequence. De novo synthesized polypeptides, employing minicells that carried some of these plasmids, allowed us to determine the direction of transcription of the glnA gene relative to an adjoining gene that codes for a 65000 dalton protein. |
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