The molecular cloning of the immunity gene of phage Mu
The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 0...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1977 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Gene - Amsterdam : Elsevier, 2(1977), 5-6, Seite 263-271 |
| Übergeordnetes Werk: |
volume:2 ; year:1977 ; number:5-6 ; pages:263-271 |
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| 520 | |a The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. | ||
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(DE-627)NLEJ187324670 (DE-599)GBVNLZ187324670 DE-627 ger DE-627 rakwb eng The molecular cloning of the immunity gene of phage Mu 1977 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Zipser, D. oth Moses, P. oth Kahmann, R. oth Kamp, D. oth in Gene Amsterdam : Elsevier 2(1977), 5-6, Seite 263-271 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:2 year:1977 number:5-6 pages:263-271 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(77)90005-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 2 1977 5-6 263-271 |
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(DE-627)NLEJ187324670 (DE-599)GBVNLZ187324670 DE-627 ger DE-627 rakwb eng The molecular cloning of the immunity gene of phage Mu 1977 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Zipser, D. oth Moses, P. oth Kahmann, R. oth Kamp, D. oth in Gene Amsterdam : Elsevier 2(1977), 5-6, Seite 263-271 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:2 year:1977 number:5-6 pages:263-271 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(77)90005-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 2 1977 5-6 263-271 |
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(DE-627)NLEJ187324670 (DE-599)GBVNLZ187324670 DE-627 ger DE-627 rakwb eng The molecular cloning of the immunity gene of phage Mu 1977 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Zipser, D. oth Moses, P. oth Kahmann, R. oth Kamp, D. oth in Gene Amsterdam : Elsevier 2(1977), 5-6, Seite 263-271 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:2 year:1977 number:5-6 pages:263-271 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(77)90005-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 2 1977 5-6 263-271 |
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(DE-627)NLEJ187324670 (DE-599)GBVNLZ187324670 DE-627 ger DE-627 rakwb eng The molecular cloning of the immunity gene of phage Mu 1977 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Zipser, D. oth Moses, P. oth Kahmann, R. oth Kamp, D. oth in Gene Amsterdam : Elsevier 2(1977), 5-6, Seite 263-271 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:2 year:1977 number:5-6 pages:263-271 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(77)90005-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 2 1977 5-6 263-271 |
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(DE-627)NLEJ187324670 (DE-599)GBVNLZ187324670 DE-627 ger DE-627 rakwb eng The molecular cloning of the immunity gene of phage Mu 1977 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Zipser, D. oth Moses, P. oth Kahmann, R. oth Kamp, D. oth in Gene Amsterdam : Elsevier 2(1977), 5-6, Seite 263-271 (DE-627)NLEJ177212578 (DE-600)1491012-3 0378-1119 nnns volume:2 year:1977 number:5-6 pages:263-271 http://linkinghub.elsevier.com/retrieve/pii/0378-1119(77)90005-1 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 2 1977 5-6 263-271 |
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The molecular cloning of the immunity gene of phage Mu |
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The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. |
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The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. |
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The 1000 base pair left-end HindIII fragment of phage Mu DNA was cloned in the EcoRI cleavage site of plasmid pMB9, using poly(dA.dT) linkers added with terminal transferase. These plasmids conferred a high level of Mu immunity on their hosts. When the plasmids were segregated into minicells, a 25 000 molecular weight protein correlated with immunity could be identified by labeling and gel electrophoresis. |
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