Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum
Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH...
Ausführliche Beschreibung
Gespeichert in:
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1964 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: BBA - Specialised Section On Biophysical Subjects - Amsterdam : Elsevier, 88(1964), 2, Seite 267-277 |
| Übergeordnetes Werk: |
volume:88 ; year:1964 ; number:2 ; pages:267-277 |
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| 520 | |a Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. | ||
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(DE-627)NLEJ187434328 (DE-599)GBVNLZ187434328 DE-627 ger DE-627 rakwb eng Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum 1964 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Terpstra, W. oth Steenbergen, C.L.M. oth in BBA - Specialised Section On Biophysical Subjects Amsterdam : Elsevier 88(1964), 2, Seite 267-277 (DE-627)NLEJ187433372 (DE-600)2209580-9 0926-6577 nnns volume:88 year:1964 number:2 pages:267-277 http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 88 1964 2 267-277 |
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(DE-627)NLEJ187434328 (DE-599)GBVNLZ187434328 DE-627 ger DE-627 rakwb eng Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum 1964 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Terpstra, W. oth Steenbergen, C.L.M. oth in BBA - Specialised Section On Biophysical Subjects Amsterdam : Elsevier 88(1964), 2, Seite 267-277 (DE-627)NLEJ187433372 (DE-600)2209580-9 0926-6577 nnns volume:88 year:1964 number:2 pages:267-277 http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 88 1964 2 267-277 |
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(DE-627)NLEJ187434328 (DE-599)GBVNLZ187434328 DE-627 ger DE-627 rakwb eng Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum 1964 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Terpstra, W. oth Steenbergen, C.L.M. oth in BBA - Specialised Section On Biophysical Subjects Amsterdam : Elsevier 88(1964), 2, Seite 267-277 (DE-627)NLEJ187433372 (DE-600)2209580-9 0926-6577 nnns volume:88 year:1964 number:2 pages:267-277 http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 88 1964 2 267-277 |
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(DE-627)NLEJ187434328 (DE-599)GBVNLZ187434328 DE-627 ger DE-627 rakwb eng Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum 1964 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Terpstra, W. oth Steenbergen, C.L.M. oth in BBA - Specialised Section On Biophysical Subjects Amsterdam : Elsevier 88(1964), 2, Seite 267-277 (DE-627)NLEJ187433372 (DE-600)2209580-9 0926-6577 nnns volume:88 year:1964 number:2 pages:267-277 http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 88 1964 2 267-277 |
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(DE-627)NLEJ187434328 (DE-599)GBVNLZ187434328 DE-627 ger DE-627 rakwb eng Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum 1964 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Terpstra, W. oth Steenbergen, C.L.M. oth in BBA - Specialised Section On Biophysical Subjects Amsterdam : Elsevier 88(1964), 2, Seite 267-277 (DE-627)NLEJ187433372 (DE-600)2209580-9 0926-6577 nnns volume:88 year:1964 number:2 pages:267-277 http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 88 1964 2 267-277 |
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influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum |
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Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum |
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Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. |
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Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. |
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Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ187434328</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707074120.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1964 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ187434328</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ187434328</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1964</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Addition of H"2O"2 to a bacterial luciferase preparation resulted in the formation of a compound with a fluoresence maximum at about 470 mμ. This compond was probably the same as the one that is formed as a result of ultraviolet irradiation of luciferase preparations after addition of FMNH"2. It is assumed to be the luminescent molecule in bacterial luminescence.The effect of a number of substances (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid, peroxidase (donor: H"2O"2 oxidoreductase, EC 1.11.1.7), tryptophan and p-chloromecuribenzoate) on both the light reaction and fluorescence increase reaction was investigated. Substances inhibiting the fluorescence increase reaction (K"3Fe(CN)"6, K"4Fe(CN)"6, ascorbic acid) likewise could inhibit the light reaction. Both effects are, at least partly, ascribed to an effect on the fluorescent group of luciferase. Light reaction inhibitors affecting other groups at the enzyme surface (p-chloromercuribenzoate) did not influence the fluorescence increase effect. Activators of the light reaction (cystine, K"4Fe(CN)"6) are suggested to act by their protective action on sulfhydryl groups or on dissociable groups, presumed to be attached to these sulfhydryl groups.A discussion of the results leads to the conclusion that the light reaction consists of at least two consecutive reactions, involving two different groups of the luciferase molecule.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Terpstra, W.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Steenbergen, C.L.M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">BBA - Specialised Section On Biophysical Subjects</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">88(1964), 2, Seite 267-277</subfield><subfield code="w">(DE-627)NLEJ187433372</subfield><subfield code="w">(DE-600)2209580-9</subfield><subfield code="x">0926-6577</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:88</subfield><subfield code="g">year:1964</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:267-277</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0926-6577(64)90182-2</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">88</subfield><subfield code="j">1964</subfield><subfield code="e">2</subfield><subfield code="h">267-277</subfield></datafield></record></collection>
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