Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence
The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles...
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1984 |
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Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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in: Plant Science Letters - Amsterdam : Elsevier, 33(1984), 2, Seite 163-175 |
| Übergeordnetes Werk: |
volume:33 ; year:1984 ; number:2 ; pages:163-175 |
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NLEJ187582963 |
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| 245 | 1 | 0 | |a Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence |
| 264 | 1 | |c 1984 | |
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| 520 | |a The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. | ||
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(DE-627)NLEJ187582963 (DE-599)GBVNLZ187582963 DE-627 ger DE-627 rakwb eng Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Weigel, H.J. oth Weis, E. oth in Plant Science Letters Amsterdam : Elsevier 33(1984), 2, Seite 163-175 (DE-627)NLEJ177035382 (DE-600)2205216-1 0304-4211 nnns volume:33 year:1984 number:2 pages:163-175 http://dx.doi.org/10.1016/0304-4211(84)90006-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 33 1984 2 163-175 |
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(DE-627)NLEJ187582963 (DE-599)GBVNLZ187582963 DE-627 ger DE-627 rakwb eng Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Weigel, H.J. oth Weis, E. oth in Plant Science Letters Amsterdam : Elsevier 33(1984), 2, Seite 163-175 (DE-627)NLEJ177035382 (DE-600)2205216-1 0304-4211 nnns volume:33 year:1984 number:2 pages:163-175 http://dx.doi.org/10.1016/0304-4211(84)90006-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 33 1984 2 163-175 |
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(DE-627)NLEJ187582963 (DE-599)GBVNLZ187582963 DE-627 ger DE-627 rakwb eng Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Weigel, H.J. oth Weis, E. oth in Plant Science Letters Amsterdam : Elsevier 33(1984), 2, Seite 163-175 (DE-627)NLEJ177035382 (DE-600)2205216-1 0304-4211 nnns volume:33 year:1984 number:2 pages:163-175 http://dx.doi.org/10.1016/0304-4211(84)90006-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 33 1984 2 163-175 |
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(DE-627)NLEJ187582963 (DE-599)GBVNLZ187582963 DE-627 ger DE-627 rakwb eng Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Weigel, H.J. oth Weis, E. oth in Plant Science Letters Amsterdam : Elsevier 33(1984), 2, Seite 163-175 (DE-627)NLEJ177035382 (DE-600)2205216-1 0304-4211 nnns volume:33 year:1984 number:2 pages:163-175 http://dx.doi.org/10.1016/0304-4211(84)90006-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 33 1984 2 163-175 |
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(DE-627)NLEJ187582963 (DE-599)GBVNLZ187582963 DE-627 ger DE-627 rakwb eng Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence 1984 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Weigel, H.J. oth Weis, E. oth in Plant Science Letters Amsterdam : Elsevier 33(1984), 2, Seite 163-175 (DE-627)NLEJ177035382 (DE-600)2205216-1 0304-4211 nnns volume:33 year:1984 number:2 pages:163-175 http://dx.doi.org/10.1016/0304-4211(84)90006-3 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 33 1984 2 163-175 |
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determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence |
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Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence |
| abstract |
The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. |
| abstractGer |
The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. |
| abstract_unstemmed |
The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ187582963</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707080212.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1984 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ187582963</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ187582963</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Determination of the proton concentration difference across the tonoplast membrane of isolated vacuoles by means of (-amino fluorescence</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1984</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The fluorescent amine 9-aminoacridine (9-AA) has been used to investigate the proton concentration difference (ΔpH) across the tonoplast membrane of mesophyll vacuoles isolated from Valerianella locusta (L.). Fluorescence quenching was measured in dependence on the concentration of isolated vacuoles, the pH of the suspending medium and the concentration of the amine added. An intravacuolar pH of about 5.0 was calculated at an external pH of 6.9-7.6. It is suggested that binding of 9-AA molecules to membrane surfaces and/or proteins contributes only little to the fluorescence quenching. Addition of carbonylcyanide-p-trifluoromethoxy-phenyl-hydrazone (FCCP) caused little decline of the ΔpH. In the absence of ionophores the ΔpH was relatively insensitive to the addition of external salt but became highly sensitive in the presence of either valinomycin or valinomycin plus FCCP. Under these conditions a complete breakdown of the ΔpH was observed in the presence of 50 mM KCl. The results are interpreted in such a way that in isolated vacuoles the maintenance of the ΔpH is mainly based on the low permeability of the tonoplast membrane for protons, while in the presence of ionophores a donnan equilibrium is established. The main advantages of the 9-AA method are: (i) separation of the vacuoles from the suspension medium is not required and (ii) it allows rapid and continuous monitoring of changes of the ΔpH.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weigel, H.J.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Weis, E.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Plant Science Letters</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">33(1984), 2, Seite 163-175</subfield><subfield code="w">(DE-627)NLEJ177035382</subfield><subfield code="w">(DE-600)2205216-1</subfield><subfield code="x">0304-4211</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:1984</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:163-175</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1016/0304-4211(84)90006-3</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">1984</subfield><subfield code="e">2</subfield><subfield code="h">163-175</subfield></datafield></record></collection>
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7.4018183 |