Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization
Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-p...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1982 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Journal of Chromatography B: Biomedical Sciences and Applications - Amsterdam : Elsevier, 231(1982), 1, Seite 145-154 |
| Übergeordnetes Werk: |
volume:231 ; year:1982 ; number:1 ; pages:145-154 |
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| 245 | 1 | 0 | |a Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization |
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| 520 | |a Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. | ||
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(DE-627)NLEJ188274278 (DE-599)GBVNLZ188274278 DE-627 ger DE-627 rakwb eng Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wong, L.T. oth Beaubien, A.R. oth Pakuts, A.P. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 231(1982), 1, Seite 145-154 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:231 year:1982 number:1 pages:145-154 http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)80518-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 231 1982 1 145-154 |
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(DE-627)NLEJ188274278 (DE-599)GBVNLZ188274278 DE-627 ger DE-627 rakwb eng Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wong, L.T. oth Beaubien, A.R. oth Pakuts, A.P. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 231(1982), 1, Seite 145-154 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:231 year:1982 number:1 pages:145-154 http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)80518-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 231 1982 1 145-154 |
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(DE-627)NLEJ188274278 (DE-599)GBVNLZ188274278 DE-627 ger DE-627 rakwb eng Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wong, L.T. oth Beaubien, A.R. oth Pakuts, A.P. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 231(1982), 1, Seite 145-154 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:231 year:1982 number:1 pages:145-154 http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)80518-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 231 1982 1 145-154 |
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(DE-627)NLEJ188274278 (DE-599)GBVNLZ188274278 DE-627 ger DE-627 rakwb eng Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wong, L.T. oth Beaubien, A.R. oth Pakuts, A.P. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 231(1982), 1, Seite 145-154 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:231 year:1982 number:1 pages:145-154 http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)80518-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 231 1982 1 145-154 |
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(DE-627)NLEJ188274278 (DE-599)GBVNLZ188274278 DE-627 ger DE-627 rakwb eng Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization 1982 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Wong, L.T. oth Beaubien, A.R. oth Pakuts, A.P. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 231(1982), 1, Seite 145-154 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:231 year:1982 number:1 pages:145-154 http://linkinghub.elsevier.com/retrieve/pii/S0378-4347(00)80518-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 231 1982 1 145-154 |
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determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization |
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Determination of amikacin in microlitre quantities of biological fluids by high-performance liquid chromatography using 1-fluoro-2,4-dinitrobenzene derivatization |
| abstract |
Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. |
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Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. |
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Pre-column derivatization of amikacin with 1-fluoro-2,4-dinitrobenzene in 25 μl of guinea pig plasma or human serum produced a stable chromophore which was measured by UV detection after rapid separation on normal-phase or reversed-phase high-performance liquid chromatography systems. The reversed-phase system, selected for routine analysis due to instability of the normal-phase column, consisted of an Ultrasphere-ODS C18 column preceded by a guard column, and used acetonitrile-water (68:32) as the mobile phase. A high degree of linearity was found in the range of 2-64 μg/ml with a coefficient of variation averaging less than 5%. |
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