Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization
Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [d...
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1994 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Journal of Chromatography B: Biomedical Sciences and Applications - Amsterdam : Elsevier, 655(1994), 2, Seite 195-200 |
| Übergeordnetes Werk: |
volume:655 ; year:1994 ; number:2 ; pages:195-200 |
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| 245 | 1 | 0 | |a Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization |
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| 520 | |a Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. | ||
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(DE-627)NLEJ188304177 (DE-599)GBVNLZ188304177 DE-627 ger DE-627 rakwb eng Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Iwase, H. oth Ono, I. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 655(1994), 2, Seite 195-200 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:655 year:1994 number:2 pages:195-200 http://linkinghub.elsevier.com/retrieve/pii/0378-4347(94)00104-9 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 655 1994 2 195-200 |
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(DE-627)NLEJ188304177 (DE-599)GBVNLZ188304177 DE-627 ger DE-627 rakwb eng Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Iwase, H. oth Ono, I. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 655(1994), 2, Seite 195-200 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:655 year:1994 number:2 pages:195-200 http://linkinghub.elsevier.com/retrieve/pii/0378-4347(94)00104-9 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 655 1994 2 195-200 |
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(DE-627)NLEJ188304177 (DE-599)GBVNLZ188304177 DE-627 ger DE-627 rakwb eng Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Iwase, H. oth Ono, I. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 655(1994), 2, Seite 195-200 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:655 year:1994 number:2 pages:195-200 http://linkinghub.elsevier.com/retrieve/pii/0378-4347(94)00104-9 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 655 1994 2 195-200 |
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(DE-627)NLEJ188304177 (DE-599)GBVNLZ188304177 DE-627 ger DE-627 rakwb eng Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Iwase, H. oth Ono, I. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 655(1994), 2, Seite 195-200 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:655 year:1994 number:2 pages:195-200 http://linkinghub.elsevier.com/retrieve/pii/0378-4347(94)00104-9 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 655 1994 2 195-200 |
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(DE-627)NLEJ188304177 (DE-599)GBVNLZ188304177 DE-627 ger DE-627 rakwb eng Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization 1994 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Iwase, H. oth Ono, I. oth in Journal of Chromatography B: Biomedical Sciences and Applications Amsterdam : Elsevier 655(1994), 2, Seite 195-200 (DE-627)NLEJ177261676 (DE-600)1491259-4 0378-4347 nnns volume:655 year:1994 number:2 pages:195-200 http://linkinghub.elsevier.com/retrieve/pii/0378-4347(94)00104-9 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 655 1994 2 195-200 |
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determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization |
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Determination of ascorbic acid in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for precolumn deproteinization |
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Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. |
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Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. |
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Ascorbic acid (AA) was determined in human plasma by high-performance liquid chromatography with electrochemical detection using a hydroxyapatite cartridge for plasma deproteinization. The proposed method is simple, rapid (deproteinization time, within 1 min; analysis time, ca. 15 min), sensitive [detection limit, ca. 240 ng/ml plasma (at a signal-to-noise ratio of 2:1)], highly selective and reproducible [relative standard deviation, ca. 2.8% (n = 3)]. The calibration graph for AA was linear in the range 0.1-10 ng per injection (20 μl). The recovery of AA was over 90% by the standard addition method. |
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