Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies
Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidize...
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| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1987 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
|---|---|
| Übergeordnetes Werk: |
in: Journal of Pharmaceutical and Biomedical Analysis - Amsterdam : Elsevier, 5(1987), 3, Seite 275-282 |
| Übergeordnetes Werk: |
volume:5 ; year:1987 ; number:3 ; pages:275-282 |
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| 520 | |a Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. | ||
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(DE-627)NLEJ188508147 (DE-599)GBVNLZ188508147 DE-627 ger DE-627 rakwb eng Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies 1987 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Curry, S.H. oth Aburawi, S.M. oth Whelpton, R. oth in Journal of Pharmaceutical and Biomedical Analysis Amsterdam : Elsevier 5(1987), 3, Seite 275-282 (DE-627)NLEJ18483757X (DE-600)1491820-1 0731-7085 nnns volume:5 year:1987 number:3 pages:275-282 http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 5 1987 3 275-282 |
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(DE-627)NLEJ188508147 (DE-599)GBVNLZ188508147 DE-627 ger DE-627 rakwb eng Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies 1987 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Curry, S.H. oth Aburawi, S.M. oth Whelpton, R. oth in Journal of Pharmaceutical and Biomedical Analysis Amsterdam : Elsevier 5(1987), 3, Seite 275-282 (DE-627)NLEJ18483757X (DE-600)1491820-1 0731-7085 nnns volume:5 year:1987 number:3 pages:275-282 http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 5 1987 3 275-282 |
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(DE-627)NLEJ188508147 (DE-599)GBVNLZ188508147 DE-627 ger DE-627 rakwb eng Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies 1987 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Curry, S.H. oth Aburawi, S.M. oth Whelpton, R. oth in Journal of Pharmaceutical and Biomedical Analysis Amsterdam : Elsevier 5(1987), 3, Seite 275-282 (DE-627)NLEJ18483757X (DE-600)1491820-1 0731-7085 nnns volume:5 year:1987 number:3 pages:275-282 http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 5 1987 3 275-282 |
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(DE-627)NLEJ188508147 (DE-599)GBVNLZ188508147 DE-627 ger DE-627 rakwb eng Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies 1987 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Curry, S.H. oth Aburawi, S.M. oth Whelpton, R. oth in Journal of Pharmaceutical and Biomedical Analysis Amsterdam : Elsevier 5(1987), 3, Seite 275-282 (DE-627)NLEJ18483757X (DE-600)1491820-1 0731-7085 nnns volume:5 year:1987 number:3 pages:275-282 http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 5 1987 3 275-282 |
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(DE-627)NLEJ188508147 (DE-599)GBVNLZ188508147 DE-627 ger DE-627 rakwb eng Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies 1987 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Curry, S.H. oth Aburawi, S.M. oth Whelpton, R. oth in Journal of Pharmaceutical and Biomedical Analysis Amsterdam : Elsevier 5(1987), 3, Seite 275-282 (DE-627)NLEJ18483757X (DE-600)1491820-1 0731-7085 nnns volume:5 year:1987 number:3 pages:275-282 http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 5 1987 3 275-282 |
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analysis of solutions containing glutathione and inorganic nitrite: application to nitroglycerin metabolism studies |
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Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies |
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Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. |
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Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. |
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Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ188508147</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506101548.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">070506s1987 xx |||||o 00| ||eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ188508147</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVNLZ188508147</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of solutions containing glutathione and inorganic nitrite: Application to nitroglycerin metabolism studies</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1987</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Nitroglycerin (GTN) is metabolized to 1,2-dinitroglycerin (1,2-GDN) and 1,3-dinitroglycerin (1,3-GDN) in vivo and in liver homogenates. 1,2-GDN and 1,3-GDN are converted to isomers of glyceryl mononitrate (GMN) in vivo. The denitration reactions yield inorganic nitrite (NO^-"2) which is oxidized to inorganic nitrate (NO^-"3). Denitration involves utilization of glutathione (GSH). In attempting to use the Bratton-Marshall assay for NO^-"2 in studies of GTN metabolism in vitro, and in attempting to use Ellman's reagent for GSH in the same research, apparent concentrations of both NO^-"2 and GSH were noticed lower than anticipated. Apparent mutual interference by NO^-"2 and GSH in their respective assays was then found. Development of a specific liquid chromatographic method for measurement of NO^-"2, NO^-"3, GSH and oxidized glutathione (GSSG) permitted the study of the interaction of NO^-"2 and GSH, which yielded NO^-"3 and GSSG.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Elsevier Journal Backfiles on ScienceDirect 1907 - 2002</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Curry, S.H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Aburawi, S.M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Whelpton, R.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">in</subfield><subfield code="t">Journal of Pharmaceutical and Biomedical Analysis</subfield><subfield code="d">Amsterdam : Elsevier</subfield><subfield code="g">5(1987), 3, Seite 275-282</subfield><subfield code="w">(DE-627)NLEJ18483757X</subfield><subfield code="w">(DE-600)1491820-1</subfield><subfield code="x">0731-7085</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:5</subfield><subfield code="g">year:1987</subfield><subfield code="g">number:3</subfield><subfield code="g">pages:275-282</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://linkinghub.elsevier.com/retrieve/pii/0731-7085(87)80031-6</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_H</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-SDJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">5</subfield><subfield code="j">1987</subfield><subfield code="e">3</subfield><subfield code="h">275-282</subfield></datafield></record></collection>
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7.401434 |