Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer
Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1992 |
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| Reproduktion: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
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| Übergeordnetes Werk: |
in: Theriogenology - Amsterdam : Elsevier, 38(1992), 3, Seite 501-511 |
| Übergeordnetes Werk: |
volume:38 ; year:1992 ; number:3 ; pages:501-511 |
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NLEJ188953051 |
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| 520 | |a Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. | ||
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| 700 | 1 | |a Sparks, A.E.T. |4 oth | |
| 700 | 1 | |a Canseco, R.S. |4 oth | |
| 700 | 1 | |a Knight, J.W. |4 oth | |
| 700 | 1 | |a Johnson, J.L. |4 oth | |
| 700 | 1 | |a Velander, W.H. |4 oth | |
| 700 | 1 | |a Page, R.L. |4 oth | |
| 700 | 1 | |a Drohan, W.N. |4 oth | |
| 700 | 1 | |a Kornegay, E.T. |4 oth | |
| 700 | 1 | |a Pearson, R.E. |4 oth | |
| 700 | 1 | |a Wilkins, T.D. |4 oth | |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 DE-627 ger DE-627 rakwb eng Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Williams, B.L. oth Sparks, A.E.T. oth Canseco, R.S. oth Knight, J.W. oth Johnson, J.L. oth Velander, W.H. oth Page, R.L. oth Drohan, W.N. oth Kornegay, E.T. oth Pearson, R.E. oth Wilkins, T.D. oth Gwazdauskas, F.C. oth in Theriogenology Amsterdam : Elsevier 38(1992), 3, Seite 501-511 (DE-627)NLEJ176881344 (DE-600)1498777-6 0093-691X nnns volume:38 year:1992 number:3 pages:501-511 http://dx.doi.org/10.1016/0093-691X(92)90070-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1992 3 501-511 |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 DE-627 ger DE-627 rakwb eng Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Williams, B.L. oth Sparks, A.E.T. oth Canseco, R.S. oth Knight, J.W. oth Johnson, J.L. oth Velander, W.H. oth Page, R.L. oth Drohan, W.N. oth Kornegay, E.T. oth Pearson, R.E. oth Wilkins, T.D. oth Gwazdauskas, F.C. oth in Theriogenology Amsterdam : Elsevier 38(1992), 3, Seite 501-511 (DE-627)NLEJ176881344 (DE-600)1498777-6 0093-691X nnns volume:38 year:1992 number:3 pages:501-511 http://dx.doi.org/10.1016/0093-691X(92)90070-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1992 3 501-511 |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 DE-627 ger DE-627 rakwb eng Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Williams, B.L. oth Sparks, A.E.T. oth Canseco, R.S. oth Knight, J.W. oth Johnson, J.L. oth Velander, W.H. oth Page, R.L. oth Drohan, W.N. oth Kornegay, E.T. oth Pearson, R.E. oth Wilkins, T.D. oth Gwazdauskas, F.C. oth in Theriogenology Amsterdam : Elsevier 38(1992), 3, Seite 501-511 (DE-627)NLEJ176881344 (DE-600)1498777-6 0093-691X nnns volume:38 year:1992 number:3 pages:501-511 http://dx.doi.org/10.1016/0093-691X(92)90070-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1992 3 501-511 |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 DE-627 ger DE-627 rakwb eng Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Williams, B.L. oth Sparks, A.E.T. oth Canseco, R.S. oth Knight, J.W. oth Johnson, J.L. oth Velander, W.H. oth Page, R.L. oth Drohan, W.N. oth Kornegay, E.T. oth Pearson, R.E. oth Wilkins, T.D. oth Gwazdauskas, F.C. oth in Theriogenology Amsterdam : Elsevier 38(1992), 3, Seite 501-511 (DE-627)NLEJ176881344 (DE-600)1498777-6 0093-691X nnns volume:38 year:1992 number:3 pages:501-511 http://dx.doi.org/10.1016/0093-691X(92)90070-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1992 3 501-511 |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 DE-627 ger DE-627 rakwb eng Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer 1992 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 Williams, B.L. oth Sparks, A.E.T. oth Canseco, R.S. oth Knight, J.W. oth Johnson, J.L. oth Velander, W.H. oth Page, R.L. oth Drohan, W.N. oth Kornegay, E.T. oth Pearson, R.E. oth Wilkins, T.D. oth Gwazdauskas, F.C. oth in Theriogenology Amsterdam : Elsevier 38(1992), 3, Seite 501-511 (DE-627)NLEJ176881344 (DE-600)1498777-6 0093-691X nnns volume:38 year:1992 number:3 pages:501-511 http://dx.doi.org/10.1016/0093-691X(92)90070-8 GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE AR 38 1992 3 501-511 |
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Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer |
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(DE-627)NLEJ188953051 (DE-599)GBVNLZ188953051 |
| title_full |
Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer |
| journal |
Theriogenology |
| journalStr |
Theriogenology |
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eng |
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marc |
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1992 |
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501 |
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38 |
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Elektronische Aufsätze |
| title_sort |
evaluation of systems for collection of porcine zygotes for dna microinjection and transfer |
| title_auth |
Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer |
| abstract |
Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. |
| abstractGer |
Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. |
| abstract_unstemmed |
Crossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.6 mg altrenogest (ALT)/day for 15 to 19 days followed by superovulation with 1500 IU pregnant mares serum gonadotropin (PMSG) and 500 IU hCG (LALT); 3) gilts between 11 and 16 days of the estrous cycle received 17.6 mg ALT for 5 to 9 days and PMSG and hCG were used to induce superovulation (SALT); and 4) precocious ovulation was induced in prepubertal gilts with PMSG and hCG (PRE). A total of 505 DNA microinjected embryos transferred into 17 recipients produced 7 litters and 50 piglets, of which 8 were transgenic. The NAT sows had less (P < 0.05) ovarian activity than gilts synchronized and superovulated by all the other procedures. Synchronization treatments with PMSG did not differ (P > 0.05) in the number of corpora hemorrhagica or unovulated follicles, but SALT and PRE treaments had higher ovulation rates than LALT (24.7 +/- 2.9, 24.3 +/- 1.8 vs 11.6 +/- 2.7 ovulations; X +/- SEM). The SALT and PRE treatments yielded 12.3 +/- 2.6 and 17.7 +/- 1.7 zygotes. Successful transgenesis was accomplished with SALT and PRE procedures for estrus synchronization and superovulation. |
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GBV_USEFLAG_H ZDB-1-SDJ GBV_NL_ARTICLE |
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3 |
| title_short |
Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer |
| url |
http://dx.doi.org/10.1016/0093-691X(92)90070-8 |
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true |
| author2 |
Williams, B.L. Sparks, A.E.T. Canseco, R.S. Knight, J.W. Johnson, J.L. Velander, W.H. Page, R.L. Drohan, W.N. Kornegay, E.T. Pearson, R.E. Wilkins, T.D. Gwazdauskas, F.C. |
| author2Str |
Williams, B.L. Sparks, A.E.T. Canseco, R.S. Knight, J.W. Johnson, J.L. Velander, W.H. Page, R.L. Drohan, W.N. Kornegay, E.T. Pearson, R.E. Wilkins, T.D. Gwazdauskas, F.C. |
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| up_date |
2024-07-05T23:39:12.708Z |
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